...105669 pAAV-CKIIa-stGtACR2-FusionRed CaMKII GtACR2 (soma-targeted) FusionRed Constitutive 1, rg* Yizhar...105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed Syn GtACR2 (soma-targeted) FusionRed Cre dependent 1, 5, rg* Yizhar...105678 pAAV_hSyn1-SIO-stGtACR1-FusionRed Syn GtACR1 (soma-targeted) FusionRed Cre dependent 5 Yizhar 109048...

...stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9...cytosine base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor...have been made by optimizing expression of the fusions, modifying the linker region between Cas variant...deaminase to adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase...CRISPR-X from the Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). ...

... Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Marker PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB.... pyogenes Yang 47024 pUC gRNA Shuttle Mt U6.6 inFusion none S. pyogenes Parrott 50591 pCBC-DT2T3 see ...

... Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Fluorescent Protein Kits Blog: When... and/or function of your protein of interest by fusing it to a fluorescent protein. FRET Find FRET pairs...resides by using a well-characterized fluorescent fusion protein. In Vivo Imaging Image with these powerful...FRET based biosensors - Pertz Fluorescent Protein Fusion Construct Set - Hamdoun Browse All Plasmid Kits...

...AAV1 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...AAV9 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons...pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal...expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation Viviana Gradinaru...

...Uncx Mouse Mouse IgG2a 114547 Anti-Mitofusin-2 [N171/17.4R] Mitofusin-2 Mouse Mouse IgG2a 114548 Anti-Mortalin...SNAPAP Human Mouse IgG2a 140072 Anti-Mitofusin-1 [N111/24R] Mitofusin-1 Mouse Mouse IgG2a 140073 Anti-Pannexin...Mouse IgG2a 225377 Mitofusin-1 (Mus musculus) recombinant monoclonal antibody. Mitofusin-1 Mouse Mouse IgG2a...IgG2a 225378 Mitofusin-1 (Mus musculus) recombinant monoclonal antibody. Mitofusin-1 Mouse Mouse IgG2a.../Tmem90b Rat Mouse 211603 Mitofusin-1 scFv [N111/24] N111/24 scFv Mitofusin-1 Rat Mouse 211604 Botch scFv...Hybridoma-derived V L and V H domain PCR products are fused to a joining fragment comprising a κ light chain...heavy chain across the ER membrane. PCR-mediated fusion of these three elements is followed by their insertion...

...CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...

...transactivator (tTA) was developed. tTA was created by fusing tetR with the C-terminal domain of VP16 (virion...etracycline-controlled t rans a ctivator) was created by fusing rTetR with VP16. The tetracycline on system is ...CMV-driven; linked via IRES to EGFP-Blasticidin fusion; pMA2641 has rtTA driven by retroviral LTR promoters...than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible gene expression...

...dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation...mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes...

...TACTAGAG or TACTAG Features: 8 bp Scar; No In-Frame Fusions; Coding Prefix alleviates RBS-CDS issues. Prohibited...6 bp scar encodes Ala-Ser; Allows for in-frame fusions; NheI is rare in E. Coli genome. Prohibited Restriction... 10 w/ start and stop codons; Creates in-frame fusions w/ linker Thr-Gly; Use N-parts prefix to preserve...

... Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB...

...library is fused to the N-terminal portion of split mCherry, while a phosphobinding domain is fused to the...expressing and purifying the phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite...

...variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance... of pULTRA. Moore 19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd...pLV-mCherry 3rd CMV driven expression of mCherry fusion. See plasmids 36083 and 36086 for other FP variants...

...Coronavirus Class I Fusion Protein Upstream of Rather than Adjacent to the Fusion Peptide . TACE - Heurich...SARS-CoV Spike protein, activating its membrane fusion function.( 5 ) TACE - (ADAM17) a metalloprotease...

... RNA (sgRNA), which is derived from a synthetic fusion of the CRISPR RNA (crRNA) and the trans-activating...components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2 RNA aptamers at the...This is a AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return...

...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs).... Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates...

...Davidson lab fluorescent protein fusion constructs and backbones....with different fluorescent tags for you to create fusion proteins with your gene of interest. Please visit...

...Posts Using catalytically inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have...microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers, which recruit...

...DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or gRNA for efficient purification. Various...antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin acceptor site to dCas9 and co-expressing...

...pAAV-EF1a-mCherry-IRES-Flpo EF1a mCherry (not a fusion tag) 1, rg* Deisseroth 55637 pAAV-EF1a-Flpo EF1a... pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion tag) 8, rg* Deisseroth 55636 pAAV-EF1a-Cre EF1a...