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  1. Antibody Guide

    Type
    Collection
    ...using sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process...may need additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals...housekeeping genes). This allows for relative comparison of expression between different samples, by normalizing...normalizing protein expression to the controls before comparison. Alternatively, the samples can be normalized...ChIP protocols use enzyme digestion instead of sonication. This approach is gentler but results in non-...isolated and analyzed. Validation relies on size comparison of peptides. Independent antibody validation ...

  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Structure Plasmids mCrimson3 588 615 - Monomer mCrimson3-N1 - Mammalian Expression mCrimson3-C1 - Mammalian...FRET Biosensors Subcellular localization Michael Davidson Collection Blog: Which FP Should I Use? Blog: ...Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many N- and C-terminal... GFP - C-terminal GFP for bacterial expression Davidson Lab Plasmids - Includes many fluorescent proteins...

  3. Rett Syndrome

    Type
    Collection
    ...Link opens in a new window) PMID: 17289941 2010 - Allyson Muotri's lab develops the first iPSC model for ...window) PMID: 26944080 (Link opens in a new window) Alysson Muotri Q83X C247T Q83X M Fibroblasts & iPSC (Link...window) PMID: 26944080 (Link opens in a new window) Alysson Muotri c.806delG 806delG G269Afs*20 M Fibroblasts...patient-derived iPSCs (Link opens in a new window) Jackson Labs - mouse line developer and repository, and...Pediatr Neurol . 52, 585-591.e2. PMID: 25801175 Tillotson and Bird. 2019. The Molecular Basis of MeCP2 Function...

  4. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ... Collections MJFF Parkinson's Plasmid Resource Michael J. Fox Foundation Parkinson's Disease Plasmid Resource...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation... researchers to assemble and share tools for Parkinson's Disease research. This collection is part of ...

  5. Genetic Code Expansion

    Type
    Collection
    ...synthetase M. maripaludis phosphoserine Bacterial Jason W Chin 174078 pDule-3-nitroTyrosine (A7) 3NY (A7...MmPylRS_MmtRNA-Pyl-opt(UGA) PylRS M. mazei Bacterial TGA Jason W Chin 174516 pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe... AfTyrRS CbzK and p-I-Phe Bacterial TCG and TAG Jason W Chin 174718 pRSF-G1mCNPRS G1mCNPRS M. archaeon...coumarin-yl) ethylglycine Bacterial TAG Kenneth Johnson 198323 pRSF-G1TMSNKRS M. archaeon TMSNK Bacterial..., TCA, or TAG codons in all open reading frames Jason W Chin 174514 Syn61Δ3(ev5) No TCG, TCA, or TAG codons... codons. Deletion of serT, serU, and prfA genes Jason W Chin 189857 Syn61Δ3(ev5) ΔrecA (ev1) No TCG, TCA...

  6. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Total gRNAs Adamson DNA Repair CRISPRi Libraries 177663, 177664, 177670 Inhibition Human Adamson 3rd ∼3 366... 131625 Inhibition E. coli Bikard N/A ∼5 21,417 Bison sgRNA Library 169942 Knockout Human Ebert 3rd 4 ...Tan 2nd 4 on average 94,000 Bradley Human CRISPR Poison Exon Knockout Library 138084 Knockout Human Bradley... 227707 227708 Prime Editing Human Human Mouse Adamson 3rd 1-20 per edit Varies CRASP-Seq Gene KO Library...

  7. Chemogenetics AAV Preps

    Type
    Collection
    ..., 9 Sternson 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson 119744...-GFP hM4D(Gi) - Inhibition GFP Cre-dependent 1 Sternson 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA ... EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson 121538 pAAV SYN1 HA-hM4D(Gi) hM4D(Gi) - Inhibition...

  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...patient-specific or disease-specific cells. For this reason, iPSCs have become an excellent unlimited stem ...Cells. Science. 2007 Dec 21;318(5858):1917-20. Thomson Lentivirus Human Doxycycline-inducible expression...piggyBac Mouse Doxycycline-inducible piggyBac transposon reprogramming system; expression of mouse Oct4... pii: cells9112451. doi: 10.3390/cells9112451. Ottosson Stem Cell Research Plasmids This table contains...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...permuted fluorescent protein (cpFP) or Förster resonance energy transfer (FRET) efficiency of a pair of...locations. PLoS One. 2012;7(11):e49371. Direct comparison of a genetically encoded sensor and small molecule... levels and metabolism (FLIPmal) Fluorescence Resonance Energy Transfer sensors for quantitative monitoring... Science. 2018 Sep 14;361(6407):1122-1126. Kai Johnsson NADPH/NADP+ or NAD+ Snifit FRET-based sensors ...dinucleotides. eLife. 2018 May 29;7. pii: 32638. Kai Johnsson Phosphate Scaffold for inorganic phosphate biosensor...Am Chem Soc. 2012 Nov 21;134(46):19026-34. Kai Johnsson Glutamate Third generation glutamate sensors (...construct custom biosensors. The ScEnSor Kit from the Olsson laboratory includes eight fluorescent reporters...

  10. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Berndt A, Grosenick L, Zalocusky KA, Bernstein H, Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve ...opens in a new window) Wick ZC, Tetzlaff MR, Krook-Magnuson E. 2019. A novel population of long-range inhibitory...Tzortzi O, Weglage M, Märtin A, Xuan Y, Parent M, Johansson Y, Fuzik J, Fürth D, Fenno LE, Ramakrishnan C,...Hyun M, Umadevi Venkataraju K, Straub C, Wang W, Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal...

  11. Caltech Systemic Capsids

    Type
    Collection
    ... CMV EGFP Control Wilson 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control Wilson 114469 pAAV-CaMKIIa-mCherry...Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson 107738 pAAV-hSyn-...

  12. The Pleiades Promoter Project

    Type
    Collection
    ...Schmouth JF, Swanson MI, Tam B, Ticoll A, Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK...Additional Resources References Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui SJ, Fulton... Jones SJ, Holt RA, Goldowitz D, Wasserman WW, Simpson EM. A regulatory toolbox of MiniPromoters to drive...

  13. Depositor Collections

    Type
    Collection
    ...Collection Visualization Michael Davidson Fluorescent Protein Collection - Davidson Allen Institute for Cell ...mini-promoters to drive selective expression in the brain - Simpson Reagents for improved expression of recombinant...

  14. Fluorescent Protein Guide

    Type
    Collection
    ...like transcription. Michael Davidson FP Collection Browse the Michael Davidson comprehensive collection ...UCSF Molecular Expressions Galleries from Michael Davidson and FSU Spectra Database hosted at the University...

  15. FlyCRISPR

    Type
    Collection
    ...research from the O'Connor-Giles, Wildonger, and Harrison labs.... Protocols gRNA Design Tools CRISPR Blog Posts Harrison Lab - Wildonger Lab - O'Connor-Giles Lab email..., Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J, O'Connor-Giles KM. Genetics ....

  16. Distribution to Industry

    Type
    Collection
    ...CRISPR tools Fluorescent Proteins from the Michael Davidson collection Protein Labeling from New England Biolabs...RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...

  17. Zebrafish Plasmid Collection

    Type
    Collection
    ...standard toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos... expression system for use in zebrafish - Bret Pearson Lab MAZe: a tool for mosaic analysis of gene function...digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert Pan and Alex Schier Labs....

  18. Tetracycline Inducible Expression

    Type
    Collection
    ...cells. Yao F, Svensjö T, Winkler T, Lu M, Eriksson C, Eriksson E. Hum Gene Ther . 1998 Sep 1;9(13):1939...target rtTA On Gu 60495 pSBtet-GP Sleeping Beauty transposon system; has luciferase in cloning site; see article...

  19. Biosensor AAV Preps

    Type
    Collection
    ... 1, 9 GENIE 174007 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Syn jGCaMP8s none Cre-dependent 9 Histed 176749...pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 CamKII GCaMP6f none Constitutive 1, 5, 9 Wilson 100835 pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 CAG GCaMP6f...AAV.CamKII.GCaMP6s.WPRE.SV40 CamKII GCaMP6f none Constitutive 9 Wilson 111262 pAAV-hSynapsin1-axon-GCaMP6s Syn axon-GCaMP6s...

  20. KLF Research Plasmids

    Type
    Collection
    ...Rudolf Jaenisch Keisuke Kaji Jonathan Katz Nathan Lawson Kathrin Plath Miguel Ramalho-Santos Derrick Rossi...Rossi Michael Ruppert Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka...
Showing: 21 - 40 of 70 results