...NOT Cre. B) This strategy has been expanded to incorporate VCre in order to enable three-feature cell targeting...recognition sequences enables directional control of the portion of the ORF that is sandwiched between the sites...working with INTRSECT recombinases, viruses, and important controls to consider as part of experimental design..., Poulin JF, Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission... a new window) Mingote S, Amsellem A, Kempf A, Rayport S, Chuhma N. 2020. Dopamine-glutamate neuron projections...

...carrier proteins (SLCs) are the largest family of transporters in the human genome with approximately 450 identified...agreement No 777372. This Joint Undertaking receives support from the European Union's Horizon 2020 research...overexpression library of human SLC proteins. Human Transporter Knockout Libraries 213695 221409 221410 Superti-Furga...knockout library targeting human transmembrane transporters. Feedback Please send your feedback to the Resolute...

...Addgene assists importation by including thorough shipping documents and following import procedure, it ...it is the responsibility of the importer to pay for any additional customs fees or taxes, and to complete... that might be required by your country for the import of biological research materials. Please be aware...

...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49411 pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49409 pCFD2-dU6:2gRNA dU6:2 BbsI Injection or in...transcription Virmilion none, need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, ...

...Endogenous Zebrafish Gene Targets Recent published reports have shown that engineered zinc finger nucleases... making targeted mutations in zebrafish. With support from the NIH and guidance from the academic community...Nuclease Expression Vectors for more details). IMPORTANT NOTE: All zinc finger arrays listed show transcriptional...greater in a well-characterized bacterial two-hybrid reporter system but have NOT yet been tested for activity...

...induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. Woltjen Plasmid Mouse...differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl...self-regulating and nonintegrating viral vectors. Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. Jaenisch...Cells and B Lymphocytes to Neural Stem Cells. Cell Reports. 2014 Dec 11;9(5):1673-80. Parmar Fibroblasts Astrocytes...astrocytes by defined transcription factors. Stem Cell Reports. 2015 Jan 13;4(1):25-36. Broccoli Fibroblasts Sensory...

...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...controlled Cre/loxP system that enables precise temporal and spatial control of gene expression in zebrafish...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...

...(sodium/bile acid cotransporter family), member 2 ASBT, ISBT, NTCP2 TAP1 transporter 1, ATP-binding cassette...grouped into tables based on the Gene Lists from Immport . Click the links below to go to the gene table...perforin and granzyme B, two enzymes that create pores within an infected cell membrane, triggering cell...polypeptide FCRG FCGRT Fc fragment of IgG, receptor, transporter, alpha FCRN, alpha-chain HFE hemochromatosis ...FLJ41500, PSF1, RING4, TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP...ADIPQTL1, ADPN, APM-1, APM1, GBP28, adiponectin ADIPOR1 adiponectin receptor 1 ACDCR1, CGI-45, CGI45, FLJ25385... FLJ25385, FLJ42464, PAQR1, TESBP1A ADIPOR2 adiponectin receptor 2 ACDCR2, FLJ21432, MGC4640, PAQR2 ADM...

...Selectable markers Viral expression and packaging Reporters, shRNA expression, transgenics and genome...stable cell lines in which the plasmid is incorporated into the host DNA. For mammalian cells, you ... AdEasy®-1 plasmid Back to Top Reporters, shRNA expression, transgenics and genome ...promoter strength pBV-Luc - Luciferase reporter plasmid with very low basal activity shRNA/RNAi...

...Browse plasmids supporting the evaluation of a diverse set of novel Alzheimer's disease targets....TREAT-AD centers are focused on diversifying the portfolio of drug targets in Alzheimer’s disease (AD) by...molecules for prioritized targets that are valued opportunities for further translational investment. All resources...

...whole range of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...phage attachment sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled...

...with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...

...research opportunities to their students and other members of the MCC community. They support educators...local hub of educators teaching MDH CUREs, and opportunities to mentor future MCC members. Find a regional...

...identify novel interactions or pathways that are important in cellular recognition processes. Laboratories...Babesia microti blood stage proteins P. falciparum sporozoite cell surface and secreted proteins Mouse sperm...protein interaction network and its resolution by spatiotemporal expression profiling. Martin S, Söllner C, ...

...elegans genome is well studied and hundreds of reporters have been developed and characterized which allow...for building homologous repair templates that incorporate a self-excising drug selection cassette (SEC)... elegans. - Jane Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans...

...114407 NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding... from the Allen Institute for Cell Science data portal (Link opens in a new window) , including: data ...

...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...This DNA is then used as a template to amplify a portion of the intended lentiviral insert. During this ...

...Bullock and Port pCFD2-dU6:2gRNA 49409 Fly BbsI none S. pyogenes Virmilion Bullock and Port pDCC6 59985...Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pAc-sgRNA-Cas9 49330 Drosophila BspQI yes, cut S...Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pU6-BbsI-chiRNA 45946 Drosophila BbsI none S. pyogenes...

...Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription... pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based validation assay...N- and C-terminal domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand ...

...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452...