Skip to main content
Addgene
Showing: 21 - 31 of 31 results
  1. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...some mammalian systems have been developed. Figure 1: Overview of the basic CAST mechanism. Created with...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...
  2. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...proteins in budding yeast. Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z. PubMed 30783202...Lee et al., 2013), and are available with URA3, SpHIS5 and KANMX markers. The FP together with the protothropic...
  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...available as a substrate for another round. Figure 1: Overview of prime editing. Created with BioRender.com...OsU3 pegRNA No Yiping Qi 149545 pCFD3-NS Drosophila Drosophila U6:3 pegRNA BbsI No Vermilion Norbert Perrimon...Perrimon 149546 pCFD5-NS Drosophila Drosophila U6:3 pegRNA + nicking sgRNA BbsI No Vermilion Norbert Perrimon...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...expression in mammalian systems, bacteria, plants, and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter Selectable...Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker...
  4. Caltech Systemic Capsids

    Type
    Collection
    ... Caltech and can help guide your decision. Figure 1: Decision tree for choosing your AAV capsid. Your ...tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2...for the AAV Decision Tree The AAV decision tree graphic above helps you choose the best capsid for your...
  5. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation of specific genomic regions...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...
  6. CRISPR References and Information

    Type
    Collection
    ...PDF 2.3 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9...putative target sites for S. pyogenes Cas9, S. thermophilus Cas9, or Cpf from your input sequence or list...checks for off-target binding. Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human...tropicalis and X. laevis), zebrafish, sea squirt, Drosophila, C. elegans, Arabidopsis, rice, sorghum, silkworm...checks for off-target binding. Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans , mouse, ...off-target binding and can work for S. pyogenes, S. thermophilus or N. meningitidis Cas9 PAMs. Currently supports...
  7. CRISPR Plasmids - Plants

    Type
    Collection
    ...Hyg Chen 86196 pYPQ141-ZmUbi-RZ-As Maize ubiquitin 1 none As Cpf1 Qi 91715 pKEE401 yes, cut S. pyogenes...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...
  8. Neurodegeneration Research Collection

    Type
    Collection
    ...one of three different inherited genes: Presenilin 1, Presenilin 2, and APP gene.The majority (>90%) of...collections available at Addgene. Amyotrophic Lateral Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes...
  9. COVID-19 Resources

    Type
    Collection
    ...mediating the entry of SARS-CoV and SARS-CoV-2 viruses. ( 1 ) TMPRSS2 - a serine protease that primes the SARS-CoV...transcriptase expression plasmids from the Drew Endy and Philippa Marrack Labs. Point-of-care testing for COVID-...
  10. Bacterial Expression Systems

    Type
    Collection
    ...expression inducible by red light. pCph8 50552 pLtetO-1 Anhydrotetracycline Jeffrey Tabor Anhydrotetracycline... pZE31ms2 25860 MS2 coat protein binding sites Philippe Cluzel RNA localization: Contains a 2x array of...DsRed-ms2x2 26248 MS2 coat protein binding sites Philippe Cluzel RNA localization: Contains a 2x array of...Fluorescent protein, GUS, and LacZ Derivatives avaliable Philip Poole A family of promoter probe vectors incorporating...
  11. Luciferase Plasmid Collection

    Type
    Collection
    ... Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase...expression of firefly luciferase William Kaelin 21375 pHIV-Luciferase Firefly EF1α/IRES Lentiviral expression...stability and light output Sanjiv Sam Gambhir 104587 pHIV-iRFP720-E2A-Luc Firefly EF1α Lentiviral expression...
Showing: 21 - 31 of 31 results