...reports have shown that engineered zinc finger nucleases (ZFNs) are effective tools for making targeted...the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT...open-source' engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder...mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(...

...predict gRNA targets in gene families, HDR oligonucleotide design, and batch processing for designing ...sequence or list of sequences. RGEN (RNA-guided endonuclease) Tools: Cas-OFFinder & Cas-Designer From the...Targeter Originally developed to identify zinc finger nuclease sites, this tool from the Joung Lab has been expanded...an accession number, genomic location, pasted nucleotide sequence, or a sequence text file you upload....

...Boyden Calcium GCaMP6f/s Ca sensors and separate nuclear localized dTomato fluorophore Ting Lab GCaMP6 Plasmids...3;108(18):7351-6. Amy Palmer Zinc Cytosolic or Nuclear zinc-binding FRET biosensor (Zap) New alternately...Photosynthesis-dependent H 2 O 2 transfer from chloroplasts to nuclei provides a high-light signalling mechanism. Nat...cellular concentrations of nicotinamide adenine dinucleotides. eLife. 2018 May 29;7. pii: 32638. Kai Johnsson...GRAB-HTR6 A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility. Cell... protein pH biosensor to detect concentrative nucleoside transport. J Biol Chem. 2009 Jul 31;284(31):20499...

...associated reverse complement including additional nucleotides for cloning and expression purposes. NOTE: Here... BbsI restriction enzyme ( Table 3 ). Add a G nucleotide after the CACC sequence and before the 20-mer...pX330 vector is enhanced by the inclusion of a G nucleotide after the CACC sequence. Add a C at the 3’ end...Ligation Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated...

...EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for...design tools and ordered as standard desalted oligonucleotides with the appropriate overhangs: The plasmids...quick ligation of annealed and phosphorylated oligonucleotides containing the guide target sequence: Up to...

...recognizable sub-cellular localization (membrane, nucleus, etc.) are also available Non-toxic to living cells...pSNAPf-H2B Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid...

..., additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can...or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA...

...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan...

... tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL). To repeat the knock-in...Cas to generate conditional alleles of essential nuclear and cytoplasmic proteins, which can then be depleted...articles: Kunzelmann, et al. G3 2016 Bottcher, et al. Nucleic Acids Res. 2014 The Förstemann lab has provided...

...interactions between DNA and protein. Micrococcal nuclease digestion is used to shear the DNA in these assays...specific to your assay. Most IF techniques use a nuclear stain, such as DAPI, to identify individual cells...cells. Immunofluorescence experiments often use a nuclei marker to identify individual cells and additional... PE, and APC 4-color microscopy panel: DAPI for nuclei, AF488, AF555, and AF647 Antibody Selection Selecting...

...Luciano Marraffini Bacterial expression of Cas9 nuclease gRNA. For a more comprehensive list of our bacterial...TALEN fused to an E. coli codon optimized FokI endonuclease domain. pET-Sangamo-His 40787 TALEN Gang Bao...TALEN fused to an E. coli codon optimized FokI endonuclease domain. Return to Top Visualization and Tagging...

... method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease fusions...

...these vectors also have a FLAG epitope tag and nuclear localization signal at their amino-terminus. The...Assembly TALEN Kit . Vectors encoding wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ...

...matching nucleotides, the second binds to mcherry with either 0, 11, 18, or 20 matching nucleotides. Together...

...are only able to enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally, γ-...

...centrosomes, the plasma membrane, lipid droplets, the nucleus, and mitochondria. The activity of GTPases is regulated... regulated both upstream (by GEFs, guanine nucleotide exchange factors), and downstream (by GAPs, GTPase...

...of host cellular machineries, requiring only a nuclease-deficient Cas9 (dCas9) protein and a customized.... , Jiang et al. , Hwang et al. ), is that the nuclease-deficient mutant could silence transcription on...

...site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make...Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR...

... plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding ...DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation...

...Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. New and Noteworthy: Use tau constructs...Noteworthy: Use a CRISPRi system to target alpha-synuclein. Sastre et al. Sci Rep. 2023 Oct 18. Target neural...