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Showing: 21 - 40 of 92 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... does not require a specific flanking sequence on the target RNA, making it a very flexible system. Other...rather than DNA, sometimes requiring a protospacer flanking sequence (PFS). In bacteria, Cas13 targeting also...):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide sequence...

  2. Bacterial Expression Systems

    Type
    Collection
    ... Genome Engineering Although overexpressing or knocking down a gene can tell you much about its function.... Return to Top Protein Purification Sometimes looking at a protein inside cells just isn’t enough to ...Growth of strain USO hisB- pyrF- rpoZ- on plates lacking histidine and containing 3-AT Scot Wolfe Bacterial...- pyrF- rpoZ- , this promotes growth on plates lacking His and containing 3-AT. FLIParaF.Ec-250n 18855...

  3. Plasmids for Stem Cell Research

    Type
    Collection
    ... vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Stem...non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged episomals...Stem Cell. 2012 Apr 6;10(4):465-72. Schöler Adult Skin Fibroblasts Cholinergic Neurons Lentiviral Human...Cell Stem Cell. 2015 Nov 5;17(5):543-56. Buganim Skin Fibroblasts Motor Neurons Lentiviral Human Direct...

  4. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...pAAV-AiE2150m-minBG-SYFP2-WPRE3-BGHpA AiP1379 AiE2150m SYFP2 Purkinje cells Cerebellum 208141 rAAV-3xCore2(390m)-minBglobin-SYFP2...Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith KA, Esposito L, Waters J, Thyagarajan...Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin SM, Lein E, Levi BP, Ting JT, Zeng H, Tasic B. ... C, Ko AL, Ojemann JG, Keene CD, Silbergeld DL, Sunkin SM, Gradinaru V, Horwitz GD, Zeng H, Tasic B, Lein...

  5. Distribution to Industry

    Type
    Collection
    ...RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...Recommended Applications PI Don’t See What You’re Looking For? Please let us know what you would like to ...

  6. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

  7. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Gene Vector Core (Link opens in a new window) have kindly deposited a series of lentiviral and adenoviral... College of Medicine Gene Vector Core have also kindly provided this protocol detailing the process for...gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express gene of interest...

  8. Genetic Code Expansion

    Type
    Collection
    ...Genetic Code Expansion You may also like... GCE4All: Making Genetic Code Expansion Accessible Using Phosphoserine...with multiple copies of the tRNA. Applications By making small changes in selected amino acids within a ...there are several things to consider. If you are working off of a previously established protocol, make ...PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser 140021 ...

  9. Cre-lox system

    Type
    Collection
    ...interest by removing neoR and stop sequence; used for making transgenic mice. Plasmid 15037 also contains IRES-EGFP...interest, removes Neo and stop cassette; Contains flanking arms for Rosa26 integration; See similar plasmid...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid/Article Description...fluorescent proteins, resulting in the indelible marking of mouse neurons with multiple distinct colors ...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters. However...Gaussia luciferase is secreted and more stable, making time course experiments possible, but its short... flank the MCS for improved normalization. John Atkins 167576 mpCHECK2 Firefly/Renilla Insertion of 3'...

  11. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ...

  12. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...viral proteins as a first step for many different kinds of important experiments. In an effort to speed ...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations...

  13. Plasmid Collections

    Type
    Collection
    ... page to help you find what you're looking for. If you are looking for a non-plasmid item, browse our ...

  14. Chemogenetics Plasmids

    Type
    Collection
    ...luciferase and a light-sensing optogenetic element, making a bimodal opto-chemogenetic actuator. When the ..."KORD AAV", "LMO", "Sternson", or other terms. Looking for viral preps? We also offer ready-to-use AAV...

  15. Optogenetics Plasmid Collection

    Type
    Collection
    ...engineered or identified to increase the spectral and kinetic properties as well as improve the expression and...Cre inhibition", "Boyden 2014", or other terms. Looking for viral preps? We also offer ready-to-use AAV...

  16. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand DNA...target DNA using a gRNA and recruits Cas3. After nicking the non-targeted strand, Cas3 starts unraveling...

  17. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...assistance at [email protected] . Don’t See What You’re Looking For? At this time, Addgene has made a limited number...

  18. Resolute Plasmid Collection

    Type
    Collection
    ...Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives support ...

  20. Synthetic Biology - Overview

    Type
    Collection
    ...Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas...biological systems for novel functions. Addgene is working with leading synthetic biologists around the world...
Showing: 21 - 40 of 92 results