... gRNA backbones, and constructs for endogenous tagging. Some examples of useful worm CRISPR resources,...streamlines the production of targeting vectors for tag insertion using CRISPR/Cas9 and the selection of ...Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even more elegant...Editing in Caenorhabditis elegans. A modified approach has also been developed by the Fire laboratory and described...

...KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF 107.4 KB Goldstein Nematode: gRNA design...PDF 106.7 KB Mendenhall & Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor ; additional HDR...finger nuclease sites, this tool from the Joung Lab has been expanded to identify potential DNA target sites...cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF 47.5 KB Vosshall and Matthews CRISPR/Cas9... and quantify the efficiency of the targeted mutagenesis The amplicon sequence expected after HDR can ...

...pAAV_BiSSTe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting...pAAV_BiPVe3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting...pAAV_BiPVe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting...pAAV_BiLAMP5e3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-...pAAV_BiVIPe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting...pAAV_BiCHATe27_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting...pAAV_BiSSTe10_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting...

...studies Addgene has a collection of plasmids suitable for creating individual fluorescently tagged proteins ...Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian optimized...Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest ...Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian Express a gene of interest...

...plasmids. Ginkgo Bioworks Plasmid Collection - Addgene has partnered with Ginkgo Bioworks to distribute SARS-CoV...DETECTR . Feng Zhang's lab at the Broad Institute has utilized the natural RNAse activity of the Cas13 ...UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad Institute has published information and protocols for utilizing...bioRxiv 2020.04.20.052159 . Jennifer Doudna's lab has utilized Cas12a’s non-specific ssDNA degradation ...CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information and protocols for utilizing... out this Suggest a Plasmid form. If the plasmid has been described previously we will reach out to the...Spike Receptor Binding Domain Site-Saturation Mutagenesis Libraries - Libraries of variant Spike protein...

...Fujii, 2013). In iChIP, specific genomic regions tagged with the recognition sequences of an exogenous ...Fig. 1). In enChIP, specific genomic regions are tagged with engineered DNA-binding molecules such as TAL... 63589 3xFN-Tel-TAL/pCMV-7.1 Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat 63590...63590 3xFN-Tel-TAL/pMXs-puro Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral... 63591 3xFN-Tel-TAL/pMXs-neo Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral...systems using different CRISPR orthologues and epitope tags. Fujita T, Yuno M, Fujii H. BMC Res Notes. 2018 ...sequence can be cloned into BbsI site. gRNA scaffold has efficient ver. 2 structure. 92220 pLenti_dCas9-2xAM...

...Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you ... iRFP Gradia Lab Mammalian Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab ...C. elegans Hamdoun Lab Plasmids - Set includes tagging with mCherry, Cerulean, Citrine, and eGFP pPD95... expression Yeast Thorn Lab Vectors - Includes tagging with a wide variety of colors, some specifically...Lindquist Lab Vectors - Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria Gradia...

...Fluorescent reporter for serotonin (dendrite localization tag) Lin Tian 135420-AAV1 pAAV-syn-FLEX-jYCaMP1s Yellow...AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for...AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used ...support from the BRAIN Initiative below. Support has been identified by depositors and/or when BRAIN Initiative...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn ...forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human Jonathan...

...directly to the sdAb, or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can... immune response to it. Once the immune response has developed, the antibodies are collected, tested against...epitopes on constant regions of other antibodies. This has useful research applications, and is covered in the...exist as monomers. Each antibody class therefore has a valency, or number of binding sites available per...of antibodies multiplied by two, as each antibody has two binding sites. Isotype Description Configuration... must be able to detect if and where an antibody has bound. Signaling molecules , proteins that emit a...to avoid confusing data. Check that your machine has appropriate lasers and filters for each fluorophore...

...related to embryonic stem cell use and has the major advantage that iPSCs can potentially be derived from... be used to create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection...Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-mediated expression of human Oct3/4, shp53...dosage tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent...development. Unlike other cell types, each stem cell has the potential to either remain a stem cell or become...can be faster, bypasses intermediate pluripotent stages, and can occur without cell division, all of which...mutation and minimize tumorigenicity, an inherent disadvantage of iPS cell technology. Browse Addgene's collection...

...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization... protein of interest with known markers. Addgene has assembled a collection of fluorescent fusion proteins...protein of interest with a fluorescent protein that has a different emission wavelength. For example, if ...mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-MitoGFP-HA Mitochondria MCU GFP Vamsi Mootha 49153 GFP-Mff Mitochondria-Outer...Voeltz 79802 pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc...Mothes 79806 pTag-RFP-C-h-Rab11a-c-Myc Recycling endosomes Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-...endosomes Rab11a TagBFP James Johnson 79800 pTag-RFP-C-h-Rab4a-c-Myc Recycling endosomes Rab4a TagRFP James Johnson...

...DNA for use with virus-mediated delivery has many advantages for research. Viruses facilitate the delivery...Kb 80-130 Dividing Yes Moderate * Recombinant AAV has a low frequency of target cell genome integration...

...AAV2 capsid with the N587 residue replaces with a TAG stop codon Mali 130878 pAAV-DJ-N589X AAV-DJ-N589X...-DJ capsid with the N589 residue replaced with a TAG stop codon Mali 166921 AAV-F in pAR-9 AAV-F AAV rep...Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI Do you have suggestions for other plasmids that...products that mediate AAV replication. This requirement has been circumvented with “helper-virus free systems... contaminants of adenovirus stocks. One major advantage of using AAV for research is that it is replication-limited...

...Research Cancer Research Plasmids and Resources Addgene has a large variety of resources for use in cancer signaling...vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from the Hahn and Root...

...range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy to use, and can be safely...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti...

... Neurodegeneration Research Collection Addgene has assembled the following to help you find information...Parkinson's today. The Michael J. Fox Foundation has made a number of tools (Link opens in a new window...sporadic, having no prior family history. A small percentage (5-10%) are familial ALS cases having at least...

...and pharmaceutical companies. The RESOLUTE project has received funding from the Innovative Medicines Initiative... protein thermal stabilization, and changes of voltage all contribute to assess ligand action/engagement...

... expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory element that...results in long-term transgene expression. Some disadvantages of γ-retroviruses are that they can only transduce... can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide for more information...

... delivery by an AAV. Since this starter cell now has all the viral genes present, the virus can spread...rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Wickersham Introduction to Rabies Virus-based...

...used in LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated...Enhanced CNS tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor... CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA minBetaGlobin SYFP2 Control Ting 163509 CN1839-rAAV-hSyn1-...