... CRISPR Protein Tagging CRISPR Plasmids - Protein Tagging Browse CRISPR Plasmids...CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal context...add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists of two...design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well as design and ...134.7 KB Mendenhall and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid...on endogenous genes for the isolation of native protein complexes. This vector serves as a backbone to ...2A-PuroR cassette to the C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated...

...vector resulting in shorter N- and C-terminal tal protein segments (136AA and 63AA, respectfully). This next...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...deletion from the N terminus of the wild-type TALE protein (previously shown to preserve the DNA-binding ability...plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize subcellular positioning...length human LSD1 at the carboxy-terminus of the protein. 49043 EMM67 49044 EMM71T Golden Gate TALEN 2.0...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...

...Synergistic Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation of endogenous... stemloop 2 The MS2-P65-HSF1 activation helper protein Full references are below. Pooled genome-wide human...This plasmid enables Cre/loxP recombination and fluorescence assisted sorting of cells and nuclei in addition...plasmid facilitates Cre/loxP recombination and fluorescence assisted sorting of cells and nuclei in addition... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

...tetR with the C-terminal domain of VP16 (virion protein 16), an essential transcriptional activation domain...vector; for evaluation of shRNA efficacy using fluorescence; use with pGFPns-reporter for cDNA target rtTA...Lentiviral, miRNA expression (PRIME) system for application in knockdown of gene expression at a single copy...

...intentionally change the activity of a protein (e.g. converting a DNA binding protein to a DNA cleaving enzyme) or...express the protein with and without the ncAA in the media to make sure that the full length protein is only...phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein. Ryan Mehl...of the tRNA. Applications By making small changes in selected amino acids within a protein, any alterations...Expansion Accessible Using Phosphoserine to Study Protein Phosphorylation Rinehart Lab reagents tRNA/Synthetase...standard amino acids. Expanding the Genetic Code In protein translation, an aminoacyl-tRNA synthetase (aaRS...amino acid can be incorporated using the existing protein translation machinery. To expand the genetic code...

... domain protein 1 2B28, UBXD10 ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein 2 N2DL2,...morphogenetic protein 5 MGC34244 BMP6 bone morphogenetic protein 6 VGR, VGR1 BMP7 bone morphogenetic protein 7 OP...TYROBP TYRO protein tyrosine kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I... You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories...shock 70kDa protein 1B FLJ54328, HSP70-1B, HSP70-2, HSPA1A HSPA1L heat shock 70kDa protein 1-like HSP70...HSPA2 heat shock 70kDa protein 2 HSP70-2, HSP70-3 HSPA4 heat shock 70kDa protein 4 APG-2, HS24/P52, MGC131852...

...plasmid type, mutations, and/or tags such as fluorescent protein (FP) tags. Information on genes and associated...Davidson 54924 mApple-MAPTau-C-10 MAPT mApple CMV Parkinson's, FTD Michael Davidson 54925 mApple-MAPTau-N-10...Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy, ALS...-10 MAPT mApple CMV Parkinson's, FTD Michael Davidson 55077 mCherry-MAPTau-C-10 MAPT mCherry CMV Parkinson's... hnRNPA2_LC_WT HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98658 hnRNPA2_LC_D290V HNRNPA2B1...