...ion pore domain it is coupled to. Addgene has two main types of PSAMs: 5HT3 coupled, which are activating...the signaling pathway the engineered receptor is coupled to and the specific cell type where the receptor...most widely used chemogenetic receptors and are G-coupled protein receptors that have been engineered to ...hM4D(Gi) are all based on muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins, respectively. ... KORD is derived from the κ-opoid receptor and couples to the Gi G-protein. hM3D(Gq) and rM3D(Gs) are ...Selective Effector Molecules (PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ...activating, and GlyR-coupled, which are inhibitory. Another recently developed class of tools are Luminopsins...

...Looking For? Please let us know what you would like to see available to industry scientists. Please note this...we are allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer Information...Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric...account, you can place orders through our website. Please note that all orders must be placed online through... product pages after you login to your account. Please see our Help Center for more information on pricing...information. To learn more about Addgene’s MTA process please visit our Help Center . The MTA Process: Once your...Scientist Acknowledgement (RSA). This form can be completed electronically by the scientist who will be using...

...the following three buffers. All buffers are supplemented with 0.001% pluronic F68 or 0.014% Tween 20....available in each of the above serotypes and buffers. Please click on the plasmid name for ordering information...the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol...AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye, et al. ...AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite Ling, et al...

...experiments for multiplexed targeting with Zhang lab plasmids? For multiplexing CRISPR to target multiple genome...4 of the Cong, et. al, 2013 Science paper. For example, if you want to use double nickase, you could express...want to use wildtype cas9 or double nickase). For example, if you want to target two genomic loci with wildtype... vector, for one well in 24-well plate. A good example/reference is this Cell paper: http://www.cell.com...putting in the full target site in the donor. For example, choosing target sites that span the knock-in gene...selection marker to select transfected cells (for example, plasmid with a puromycin resistance cassette, ...individual genotyping (using Sanger sequencing, for example) on each colony in order to screen for positive...

...page . Note: For a list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog...for the 20 µL sized testing sample is denoted with a .T suffix (for example, AAV1.T). The catalog ID for...for the 100 µL sized sample does not have the suffix (for example, AAV1). AAV Vectors for Serotype Testing...different serotypes. In addition to the standard 100 µL sample size, the vectors listed below are also available...include virus associated DNA (vdna), which is a sample of the purified DNA from the original plasmid that...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...Cross lab collection for more) pLexA and pACT2.2 - Yeast 2-hybrid plasmids...understanding the function of your favorite gene. For example, fusing your protein to an epitope tag, such... Tet-inducible lentiviral shRNA expression pLenti CMV Neo DEST - Lentiviral Gateway...puro - Retroviral shRNA expression pLenti CMV Puro DEST - Lentiviral Gateway... Tet-inducible lentiviral shRNA expression pLenti CMV Hygro DEST - Lentiviral Gateway...destination vector for gene expression pLenti CMV/TO Hygro - Lentiviral Gateway...expression (tet inducible) Zeocin/Bleo Mammalian pLenti X1 GFP-Zeo DEST - Lentiviral Gateway...

...permitting selective simultaneous labeling in a sample. Please see the note below for information on substrates...Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England Biolabs offers non-toxic...community. Key features: Allows for visualization of multiple proteins simultaneously with dual labeling when...proteins Single constructs are compatible with multiple applications (different fluorophores, biotin, ...website. For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag...allow for dual labeling and visualization of complementary protein function or localization. ACP and MCP...MCP-tag Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and CLIP...

... kinase, is found in two complexes, mTORC1 and mTORC2. Each complex contains a unique subunit...p53 also promotes aberrant mTORC1 activity, and multiple familial cancers are caused by inactivating mutations...for the gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each...available plasmids for the gene. For components with multiple isoforms or subunits, individual links to each...each gene page are provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor... RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras homolog enriched in brain S6K Also known...Hippel-Lindau tumor suppressor Return to top mTORC2 Complex Symbol Name AKT AKT1 AKT2 AKT3 v-akt murine thymoma...

...Davidson 54924 mApple-MAPTau-C-10 MAPT mApple CMV Parkinson's, FTD Michael Davidson 54925 mApple-MAPTau-N-10...Cookson 13318 pLenti6-DEST PINK1-V5 G309D PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-... Cookson 29412 pLenti6-DJ1-V5-E18N PARK7 V5 CMV Parkinson's Mark Cookson 29413 pLenti6-DJ1-V5-C106D PARK7...Cookson 29414 pLenti6-DJ1-V5-L166P PARK7 His, V5 CMV Parkinson's Mark Cookson 29415 pLenti6-DJ1-V5-E18D ... Cookson 29417 pLenti6-DJ1-V5-E18Q PARK7 V5 CMV Parkinson's Mark Cookson 29418 pLenti6-DJ1-V5-D149A PARK7...ALS Mohan Babu 83444 pLEX_307-SOD1WT SOD1 EF-1 alpha ALS Mohan Babu 83445 pLEX_307-SOD1E133delta SOD1 EF...Mohan Babu 83446 pLEX_307-SOD1E133insTT SOD1 EF-1 alpha ALS Mohan Babu 83447 pLEX_307-SOD1E133K SOD1 EF...

...depdent on Cre, Flp, or Vcre Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of...mitochondria Mitycam, or ER targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for...indicators based on mRuby (jRCaMP1a, jRCaMP1b) and mApple (jRGECO1a) (Constitutive or Cre-dependent) Sensitive... Cre- or Flp-dependent) Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of...Cyan-Yellow Zinc FRET sensors ZapCV2 and ZapCV5 Droplet Microfluidic Flow Cytometer For Sorting On Transient...sensors for simultaneous monitoring of Zn(2)(+) in multiple cellular locations. PLoS One. 2012;7(11):e49371...Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities. Nat Cell ...