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Showing: 1 - 20 of 31 results

  1. Lentivirus Plasmids

    Type
    Collection
    ...gRNA plasmids table and search "lenti". ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...generation lentiviral systems Viral production Frequently asked questions about lentivirus use Glossary...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...cell upon infection. When lentivirus is used for research, it is the lentiviral genome that encodes genetic...

  2. Lentiviral Prep Service

    Type
    Collection
    ...GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding...2A-Blast-WPRE) Zhang 61426 lenti MS2-P65-HSF1_Hygro MS2-P65-HSF1 Hygromycin lenti vector encoding the MS2... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled ...sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114...gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes...sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637...Resistance Purpose PI 17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector...

  3. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of Medicine Gene Vector Core Lentiviral and ...Resources Addgene Blog: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral...deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors ...infectious virus. Protocol: Lentivirus Transfer Vector Production Protocol Lentiviral Vector ID Purpose pCDH-EF1...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Viral...and Adenoviral Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and ...Vectors Within this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not...

  4. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV | Browse Lenti Packaged on Request... libraries. Viral preparation options include lentivirus and AAV. Viral...In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer preps...

  5. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase...firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral vector expressing ...examples of each in Addgene's collection: HIV-based lentiviral particles Murine leukemia-based retroviral particles...#141347) is available in standard format and as a Lentiviral prep . ID Plasmid Description Mutations Industry...Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus...neutralizing activity are listed below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase...firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...S. pyogenes Puro Zhang lenti sgRNA(MS2)_zeo backbone 61427 Mammalian/Lentiviral BsmBI none S. pyogenes...Yang pLCKO 73311 Mammalian/Lentiviral hU6 none S. pyogenes Puro Moffat lenti sgRNA(MS2)_puro backbone 73795...pyogenes URA3 Davis lenti sgRNA(MS2)_puro optimized backbone 73797 Mammalian/Lentiviral U6 none S. pyogenes...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral H1-Tet none S. pyogenes Herold lentiGuide-Crimson 70683 Mammalian/Lentiviral hU6 none...50662 Mammalian/Lentiviral See paper none S. pyogenes Blast Sabatini and Lander lentiCRISPR v2 52961 Mammalian...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...plasmid Attila Karsi 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral expression of firefly luciferase...fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB NanoLuc® Creation ...fusions using Gateway cloning. Lentiviral. Mikko Taipale 87077 pLenti6.2-Firefly-ccdB Firefly Creation ...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...luciferase Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase...Firefly Ubc Lentiviral expression of firefly luciferase Linzhao Cheng 74444 pLenti.PGK.blast-Renilla_Luciferase...Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE Firefly EF1α Lentiviral expression of firefly...

  8. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Bonifacino 173579 Lenti-sgAtm#1/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ...Venus CMV Lewy body dementia Tiago Outeiro 89643 Lenti-sgAtm/Cre ATM U6 Ataxia telangiectasia Monte Winslow...FusionRed CUP1 ALS Michihiko Kataoka 188460 pKDR-Lenti-CBh.6-SOD1 SOD1 CBh ALS Erik Dent 188572 Tet-off...LRRK2 His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia...Cookson 13318 pLenti6-DEST PINK1-V5 G309D PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-... Cookson 29412 pLenti6-DJ1-V5-E18N PARK7 V5 CMV Parkinson's Mark Cookson 29413 pLenti6-DJ1-V5-C106D PARK7...Cookson 29414 pLenti6-DJ1-V5-L166P PARK7 His, V5 CMV Parkinson's Mark Cookson 29415 pLenti6-DJ1-V5-E18D ...

  9. Plasmids for Stem Cell Research

    Type
    Collection
    ... 11. 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic lentiviral vector for the expression ...May . 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector for the expression ...1221-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for the ...2):151-9. Jaenisch Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression.... 106(1):157-62. Jaenisch Lentivirus Mouse Doxycycline-inducible lentiviral expression of mouse Oct4, ...7239):766-70. Nagy Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression...Delivery Method Species Description Article PI Lentivirus Human Expression of human Oct4, Sox2, Klf4, Myc...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Tet-inducible lentiviral shRNA expression pLenti CMV Neo DEST - Lentiviral Gateway destination...Tet-inducible lentiviral shRNA expression pLenti CMV Hygro DEST - Lentiviral Gateway destination...expression through lentiviral delivery pLenti CMV GFP DEST - Lentiviral Gateway destination...vector - Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression... shRNA expression pLenti CMV Puro DEST - Lentiviral Gateway destination vector... expression pLenti CMV/TO Hygro - Lentiviral Gateway destination vector ...inducible) Zeocin/Bleo Mammalian pLenti X1 GFP-Zeo DEST - Lentiviral Gateway destination for shRNA...

  11. Cre-lox system

    Type
    Collection
    ...12238 pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-...coexpression CMV Lentiviral Sadelain 30205 Cre-IRES-PuroR Cre and PuroR coexpression EF-1 alpha Lentiviral Kotton...-cre mCherry and Cre Lentiviral Luikart 66691 FUG-T2A-cre GFP and Cre Lentiviral Luikart 66692 pRubiC-...-iCre copGFP and iCre CB Lentiviral Oka 72257 pCDH-CB-iCre iCre CB Lentiviral Oka 73350 pAdx-CMV-iCre-... falxiparum Spielmann 86641 pLenti-hSynapsin-Cre-WPRE Cre hSyn Lentiviral Wang 86794 pCL20c-MSCV-IRES-...Eisenhoffer 131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh...Cre, Puro resistance and miRNA expression PGK Lentiviral Jacks 17736 pOG231 NLS-Cre CMV Mammalian Wahl...

  12. Mammalian RNAi Tools

    Type
    Collection
    ...for RNAi Constitutive Lentiviral Cre-regulated Lentiviral Tet-regulated Lentiviral Constitutive Retroviral...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...shRNA design and delivery. Constitutive Lentiviral shRNA Lentiviral vectors for constitutive shRNA expression...packaging system. Didier Trono Cre-regulated Lentiviral shRNA Lentiviral vectors for conditional (Cre-lox) shRNA...expression Tyler Jacks Tet-Regulated Lentiviral shRNA Lentiviral vectors for Tet-regulated shRNA expression...Resources: Human and mouse pooled shRNA libraries Lentiviral packaging and expression plasmids Background ...the popular cloning vector pLKO.1. Trono Lab Lentivectors Toolbox Tyler Jacks Lab Protocols Publications...

  13. Viral Production

    Type
    Collection
    ...AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...viral production methods and quality control for lentivirus and AAV. Viral...AAV Production Titer Quality Control Resources Lentivirus Production Titer Quality Control Resources Overview... Cell culture medium (OptiPro SFM) containing lentivirus is first cleared by low-speed centrifugation ...cells are transduced with serial dilutions of a lentiviral vector, incubated for 72 h, and genomic DNA is...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...Description Element On or off PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral reverse tetracycline-controlled...Resources: Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections Background...On or Off PI 21916 Tet-pLKO-neo 3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin... selection TetR On Wiederschain 41393 pCW57.1 Lentiviral vector for inducible expression for Gateway cloning...insert options rtTA On Root 11651 pLVUT-tTR-KRAB Lentiviral vector for inducible expression of transgene ...tTA or rtTA None On Vogelstein 25735 pSLIK-Neo Lentiviral vector for inducible expression of a miR-shRNA...third-generation rtTA On Fraser 44012 pInducer20 Lentiviral vector for shRNA expression; see article for ...

  15. Zhang Lab CRISPR Page

    Type
    Collection
    ...system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (...#61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9-VP64 with... top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for performing...with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex...complex with a 2A Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop...

  16. CRISPR Guide

    Type
    Collection
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...

  17. Plan Your Experiment

    Type
    Collection
    ...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable,...high efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector...

  18. Viral Vectors

    Type
    Collection
    ...engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses are versatile tools because of...Target Cell Genome Integration Immune Response Lentivirus Stable RNA <8 Kb 80-130 Dividing/Non-dividing...Resources Nolan Lab Retroviral Systems Trono Lab Lentivector Toolbox Guide to Generating Recombinant Adenoviruses...

  19. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...has efficient ver. 2 structure. 92220 pLenti_dCas9-2xAM Lentiviral plasmid expressing dCas9-2xAM and gRNA...gRNA cloned in Bbs I sites. 92221 pLenti_dCas9-2xAM_hIRF-1 Lentiviral plasmid expressing dCas9-2xAM and...

  20. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression... Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus...
Showing: 1 - 20 of 31 results