...EXPRESs Plasmids Human Platelet EXPRESs Plasmids for Human Platelet Receptors You may also like... Gavin Wright...Human platelet receptor proteins for use with AVEXIS interaction screening Special...expressing secreted recombinant proteins from human platelets that was created for systematic screening of extracellular...following reference for more information: A Human Platelet Receptor Protein Microarray Identifies the High...E Receptor Subunit α (FcεR1α) as an Activating Platelet Endothelium Aggregation Receptor 1 (PEAR1) Ligand...

...kinetics and mechanisms of action, regulation and post-translational modifications, and metabolic pathways...Browse a diverse set of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based... Depositor Collections MDH CUREs Community Malate Dehydrogenase CUREs Community (MCC) Collection ...Undergraduate Research Experiences (CUREs) Background The Malate Dehydrogenase CUREs Community (MCC) (Link opens...Experiences (CUREs) centered on a single enzyme, malate dehydrogenase (MDH). The MCC is a national group...support educators interested in integrating MDH-related research into their teaching laboratories. They... plasmids, faculty can begin a sustainable MDH-related CURE focusing on protein expression, structure ...

...fluorescent proteins to modulate biological activity. Early tools allow scientists to regulate transcription, ...selectively modulate gene expression. Plasmid... Plasmid Collections Fluorescent Proteins Activity Regulation Fluorescent...Fluorescent Proteins: Activity Regulation More Fluorescent Protein Resources: Empty Backbones FRET Subcellular..., and additional tools are being developed to regulate a variety of other activities. By using GFP as ...advantage of existing GFP-lines for cell-specific manipulation. Plasmids Fluorescent Protein Purpose Principal...

... of synthetic biology plasmids related to Networks and Gene Regulation...Biology Networks and Gene Regulation Synthetic Biology: Networks and Gene Regulation SynBio Resources SynBio...collection of synthetic biology plasmids related to Networks and Regulation. The collection includes both naturally...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...naturally ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and higher level ... and activators logic gates Networks and Gene Regulation Plasmids Search the table by keyword or sort ...

...MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection You may also like.....Pooled Library The Multiplexed Overexpression of Regulatory Factors (MORF) pooled library is a comprehensive...

...KI-RAS, KRAS1, KRAS2, NS3, RASK2 LAT linker for activation of T cells LAT1, pp36 LCK lymphocyte-specific ...ADRM1 adhesion regulating molecule 1 ARM1, GP110, MGC29536, Rpn13 AP3B1 adaptor-related protein complex...polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related sequence... IREL RFX5 regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated...kinase, regulatory subunit 1 (alpha) GRB1, p85, p85-ALPHA PIK3R2 phosphoinositide-3-kinase, regulatory subunit...JULF2, KPG_004, NOP9 PF4 platelet factor 4 CXCL4, MGC138298, SCYB4 PF4V1 platelet factor 4 variant 1 CXCL4L1...

...detach cells. Plate 100 μl each into two separate 96-well flat-bottom plates. Keep one plate to allow for...“bulk”) that were not plated, freeze half of the cells for future plating. Plate the other half for screening...reaction (from 3.2.1 - 3.2.2 ). Plate onto a lysogeny broth (LB) agar plate with 100 μg/ml ampicillin and...CRISPR/Cas9 constructs. Plate sorted cells individually into 96-well round-bottom plates using sorter or by...dilution at 30 cells per 96-well round-bottom plate. Optimize plating the cell type used at limiting dilution...either grow as individual cells in 96-well flat bottom plate or in a 10 cm dish at low concentration so...derived clones can be picked and moved to a flat bottom 96-well plate. Allow the bulk cells to incubate at 37...

...efficiency of Cas9 cleavage is relatively high and the efficiency of HDR is relatively low, a large portion of...modifying the methylation state of cytosines in a gene’s promoter or by inducing histone acetylation or demethylation... Select Your Desired Genetic Manipulation Different genetic manipulations require different CRISPR components...Select gene and genetic element to be manipulated In order to manipulate a given gene using CRISPR, you will...Plan Your Experiment Overview Select Genetic Manipulation Select Expression System Design gRNA Know your...has important practical implications, because a population of cells expressing Cas9 and a gRNA will result... to utilize HDR for gene editing, a DNA repair template containing the desired sequence must be delivered...

...Reagents Protein phosphorylation is one of the most abundant forms of posttranslational modifications in...questions surrounding serine phosphorylation by adding this phosphorylated amino acid to the genetic code...improving the orthogonal translation system to make singly or multiply phosphorylated proteins (Pirman et ... serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library ). The phosphorylation sites (“... ) and an optimized phosphoserine orthogonal translation system (pSerOTS-C1* (V70) 188537 ). These reagents... with the optimized phosphoserine orthogonal translation system ( 68292 ), they were able to demonstrate...central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein interactions. The...

...pmiRFP703-Clathrin Clathrin-coated vesicles Clathrin miRFP703 Vladislav Verkhusha 21741 Clathrin-LCa-EYFP...Helenius 70217 pEGFP-GFP11-Clathrin light chain Clathrin-coated vesicles Clathrin light chain GFP Bo Huang...Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles Clathrin mEmerald* Michael Davidson 79997 pmiRFP703...TdTomato IX Membrane Palmitoylation TdTomato Connie Cepko 22479 FUmGW Membrane Palmitoylation GFP Connie Cepko...Membrane Palmitoylation sequence from GAP43 EGFP Connie Cepko 61099 Lck-GFP Membrane Palmitoylation sequence... EGFP Toren Finkel 14435 mRFP-Clc Clathrin-coated vesicles Clathrin, light polypeptide mRFP Ari Helenius...Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-Rab5 Early endosomes...

...produces a colored product that accumulates over time which is relative to the amount of enzyme present...quantify relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific ... one type of antibody. Monocultures of B cells isolated from animals immunized as described above are ... regions of the antibody to be independently manipulated. Antibodies produced this way are called recombinant... used while suspended in the original media or isolated and placed in a buffer. Antibody Structure Figure...antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain...instead of modified antibodies and are not closely related to antibodies. Monobodies - Monobodies are synthetic...

...target in the HR template, you usually would need to make mutations of the HR template to avoid this donor...my HR template an issue? Yes. If you have intact protospacer+PAM sequence within the HR template, it will... that if a CRISPR is used to engineer a locus, isolate single cell colonies, then genotype them, it is...Homologous Recombination (HR) FAQs Can you summarize HR template vector design guidelines? A few notes below are...you should consider using ssDNA oligo as donor template, it's usually more efficient than plasmid donor...1ul of 10uM oligo stock for each well on 24-well plate, co-deliver into the cells with the Cas9/sgRNAs ...with Cas9/sgRNAs vector, for one well in 24-well plate. A good example/reference is this Cell paper: http...

...enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many cancers....RRAGB RRAGC RRAGD Ras-related GTP binding Raptor Also known as RPTOR; Regulatory associated protein of...arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation...determine the nutritional state of the cell and regulates the switch from anabolic to catabolic processes...Enhanced signaling through either mTORC1 or mTORC2 correlates with poor prognosis in many cancers, including...Akt and MAPK/ERK, both of which are commonly dysregulated in cancer. Loss of the tumor suppressor p53 ... caused by inactivating mutations in negative regulators of mTORC1. The hyperactivation of mTORC1 in cancer...

...(ras related) RALBP1 RalA binding protein 1 RALGDS Ral guanine nucleotide dissociation stimulator RAPGEF...have an active (GTP) and inactive (GDP) state, regulated by the addition and removal of a phosphate group...GTPases play prominent roles in signaling pathways regulating cell migration, survival, adhesion, growth, and...differentiation and survival. Ras is essential for regulating the growth of the cell, however when mutations...for clarity and does not indicate a specific relationship. The content and map for this page were generated...EIF4EBP EIF4EBP1 EIF4EBP2 EIF4EBP3 Eukaryotic translation initiation factor 4E binding protein ERBB2 Erb-b2...

...cortical interneurons under the control of the E2 regulatory element Jordane Dimidschstein 135630-AAV9 pAAV-S5E2...cortical interneurons under the control of the E2 regulatory element Jordane Dimidschstein 135630-PHPeB pAAV-S5E2...cortical interneurons under the control of the E2 regulatory element Jordane Dimidschstein 135631-AAV1 pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135631-AAV9 pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135631-PHPeB pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135632-AAV1 pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135632-AAV9 pAAV-S5E2...

...include histone acetylation by p300, histone demethylation by LSD1, cytosine methylation by DNMT3A or MQ1...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...100 bp window. This technique creates diverse populations of mutants for directed evolution. Plasmid Gene...Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed...primer binding site and the desired edits on an RT template. Plasmid Gene/Insert Promoter Selectable Marker...

...NTR 2.0/MTZ-based cell ablation system that allows sustained cell loss and ablation of previously...localization in high-throughput. MAZERATI platform - Leonard Zon Lab. A platform to rapidly model potential cancer...embryo and larvae, it is easy to culture large populations that are amenable to genetics, high-throughput...emerged as a model system for human disease and translational research, including the study of cancer, inflammation... enabling CRISPR/dCas9-interference (CRISPRi) modulation of enhancers and non-coding RNAs in zebrafish...organism lineage tracing by combinatorial and cumulative genome editing - Jay Shendure Lab Simultaneous...droplet reporters - Richard White Lab. An imaging platform to study lipid dynamics in vivo. Bimolecular fluorescence...

... of treatments and cures for Rett Syndrome and related MECP2 disorders. The RSRT has a comprehensive, ...diagnostic criteria, defined as the presence of relatively normal early development, regression with a loss...The MECP2 protein is a global transcriptional regulator of thousands of genes and studies have suggested...is not clear, however, the ability to bind to methylated DNA and recruit known co-repressors or other ...modifiers suggests the major function of MECP2 is to regulate gene expression either locally or globally. The...NID demonstrating the importance of binding to methylated DNA and the recruitment of the NCoR complex for... MECP2 null alleles or common mutations that recapitulate Rett syndrome in humans. MECP2 knockout rats...

...through both phosphorylation and acetylation, which disrupt its interaction with negative regulators, increase...Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related cysteine peptidase CASP9...allow it to bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional...for clarity and does not indicate a specific relationship. p53 Pathway Color is used for clarity and does... does not indicate a specific relationship. The content for this page was generated with the help of .... CASP9 Caspase 9, apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3 Cyclin B1, 2, or 3 Cyclin...proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-...

...interneurons under the control of the E2 regulatory element Viral manipulation of functionally distinct interneurons...calcium sensors for imaging activity in neuronal populations and microcompartments. Nat Methods. 2019 Jul;...GCaMP6f Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator. Neuron...), diacylglycerol and STIM1 signalling system regulates directed cell migration. Nat Cell Biol. 2014 Feb... enhancer A viral strategy for targeting and manipulating interneurons across vertebrate species. Nat ...) Improved methods for marking active neuron populations. Nat Commun. 2018 Oct 25;9(1):4440. Eric Schreiter...ER-targeted CEPIA1er ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in mammalian neurons. Science....