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  1. Church Lab CRISPR Plasmids

    Type
    Collection
    ...hCas9 A human codon-optimized Cas9 expression plasmid 41816 hCas9 D10A A human codon-optimized Cas9 expression...Description 43802 p414-TEF1p-Cas9-CYC1t A human codon-optimized Cas9 for expression in S. cerevisiae (budding...promoter 43804 p415-GalL-Cas9-CYC1t A human codon-optimized Cas9 for expression in S. cerevisiae (budding...All genes except the SP nuclease are human codon optimized but function well in E. coli . Bacteria expressing...expression, human optimized 48670 M-NMcas Mammalian N. meningitidis Cas9 expression, human optimized 48673 M-NM-sgRNA...Cas9 proteins includes three orthogonal variants optimized for use in human cells and four orthogonal variants...nuclease-null Cas9 activator expression, human optimized 48676 M-NMn-VP64 Mammalian NM-VP64 nuclease-null...

  2. Huang Lab CRISPR Plasmids

    Type
    Collection
    ...gRNA Design Tools CRISPR Blog Posts A human codon-optimized Cas9 nuclease expression plasmid. The arrangement... region was optimized for the most efficient localization of the nuclease. This optimized hCas9 expression...44758 pST1374-NLS-flag-linker-Cas9 : A human codon-optimized Cas9 expression plasmid, specifically designed...

  3. Qi Lab CRISPR Page

    Type
    Collection
    ...plasmid ( pdCas9_humanized ) contains a human codon optimized dcas9 gene under the control of Murine Stem...-humanized A catalytically inactive, human codon-optimized Cas9 expression plasmid 44247 pdCas9::BFP-humanized...-humanized A catalytically inactive, human codon-optimized Cas9-BFP fusion expression plasmid 44248 pgRNA-humanized...expression plasmid for use with either human codon-optimized Cas9 constructs 44249 pdCas9-bacteria A catalytically...

  4. Doudna Lab CRISPR Plasmids

    Type
    Collection
    ... different bacteria as well as a mammalian codon-optimized Cas9 expression plasmid. These plasmids are...aeruginosa Csy4 H29A. 42234 pMJ920 : Human codon-optimized Cas9 expression plasmid....

  5. CRISPR-Cas/RGN expression plasmids for human cells

    Type
    Collection
    ...addition of the 3X FLAG tag. JDS246 has been codon optimized for expression in mammalian cells. The guide...-term 3X FLAG tag) 43861 JDS246 (mammalian codon-optimized Cas9 with C-term 3X FLAG tag)...

  6. FlyCRISPR

    Type
    Collection
    ...below: ID Plasmid 46294 pBS-Hsp70-Cas9 : A codon-optimized Cas9 nuclease under the control of the Drosophila...Drosophila hsp70 promoter. 45945 pHsp70-Cas9 : The codon-optimized Cas9 nuclease under the control of the Drosophila...website contains additional information CRISPR Optimal Target Finder Identifies gRNA target sequences ...

  7. Zhang Lab CRISPR Page

    Type
    Collection
    ... contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single guide RNA...contains a single cassette expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR ..., Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ordered via the links...from S. aureus (SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its ... contain two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus...contains a single cassette expressing human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected ...contains a single cassette expressing human codon-optimized SpCas9, driven by the truncated MeCP2 promoter...

  8. Dimeric CRISPR RNA-guided FokI Nuclease (RFN)

    Type
    Collection
    ...have been codon optimized for expression in human cells and the dCas9 domain has been optimized for expression...encoded by this plasmid is the one that showed optimal activity in human cells as described in Tsai et...multiplex gRNA expression plasmid) 53369 pSQT1601 (optimized Csy4 and FokI-dCas9 expression vector)...

  9. Calarco Lab CRISPR Plasmids

    Type
    Collection
    ... 46168 Peft-3::cas9-SV40_NLS::tbb-23'UTR : codon optimized Cas9_SV40 NLS with intron. 46169 PU6::unc-119...CRISPR Blog Posts A CRISPR/Cas system designed and optimized for work in C. elegans . The protocol for customizing...

  10. Cre-lox system

    Type
    Collection
    ...pBF3038 Cre codon optimized for yeast Gal1 Yeast Sandmeyer 26853 pBF3060 Cre codon optimized for yeast ...enables visualization of Cre expression. Optimized Cre: Codon-optimized Cre ( iCre ) is expressed at higher...insulin Zebrafish Stainier 24593 AAV-pgk-Cre codon optimized Cre PGK AAV Aebischer 24704 GFAP-Cre Cre GFAP... Cre with stop codon Entry vector Ko 139546 pENTR-CreStopDeleted Cre without stop codon Entry vector Ko... ways to tightly control Cre expression and to optimize Cre expression once it is induced. Addgene has... mutant TEF3 Yeast Piper 61391 pCS2-Cre.zf1 Cre codon modified for zebrafish CMV Zebrafish Burgess 61570... Ko 139547 pENTR-CreERT2 Cre-ERT2 with stop codon Entry vector Ko 139670 pTFUbiCre Cre Maize Ubiquitin...

  13. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...of recoded E.coli (rEcoli XpS 192872 ) and an optimized phosphoserine orthogonal translation system (pSerOTS-C1... recoded strain of E. coli ( 68306 ) with the optimized phosphoserine orthogonal translation system ( ... and a new pSerOTS Described in: System-wide optimization of an orthogonal translation system with enhanced...Improved Reagents (ca. 2015) Described in: A flexible codon in genomically recoded Escherichia coli permits ...Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion. Heinemann...

  14. Kamoun Lab CRISPR Plasmids

    Type
    Collection
    ...CRISPR Blog Posts A CRISPR/Cas system designed and optimized for work in the model plant Nicotiana benthamiana...Plasmid 46965 pK7WGF2::hCas9 : Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823...

  15. Resolute Plasmid Collection

    Type
    Collection
    ... Plasmids This plasmid collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible...

  16. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...infectious particle production, including codon optimization, deletion of the C-terminal 18-21 amino acids...

  19. Bacterial Expression Systems

    Type
    Collection
    ...N-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. pET-Sangamo-His 40787...C-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. Return to Top Visualization...His-tagged cyan fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent...His-tagged yellow fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent ...

  20. Antibody Plasmid Collection

    Type
    Collection
    ...include consistency between lots and the ability to optimize the antibody’s antigen binding sequence to improve...which circumvents the problem of in-frame amber codons in scFvs A new expression vector facilitating production...
Showing: 1 - 20 of 26 results