...directly transfect cloning grade DNA into cells? Cloning grade DNA is designed for use in cloning applications... analyses are performed on my cloning grade DNA order? For each cloning grade DNA prep, the plasmid sequence...like to see available as cloning grade DNA, please let us know using the Cloning Grade DNA Suggestion Form... ordering cloning grade DNA for over 200 of our most popular plasmids! DNA... DNA Service: Cloning Grade DNA ...Details Plasmids Available FAQ Addgene is offering Cloning Grade DNA (cgDNA) for over 200 plasmids in our ...variety of popular plasmids and backbones with high cloning potential. By making these plasmids available as...

...related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems....Biology plasmids for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning and Genomic Tools...Tools Synthetic Biology: Cloning and Genomic Tools SynBio Resources SynBio Home Browse All SynBio Assembly...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration Recombination...Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table by keyword or sort by...

...CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a single...deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning kit) into...one plate to allow for clones to grow and use the other plate to screen each clone for deletions. Add an...Validation of Biallelic Deletion Clones In order to characterize obtained clones and validate a successful knockout...present a simple methodology for CRISPR design, cloning, and delivery for the production of genomic deletions...within exons will enrich even monoallelic deletion clones for loss of function. This is due to the high frequency...complement including additional nucleotides for cloning and expression purposes. NOTE: Here we discuss ...

...gRNA design and cloning gRNA cloning vector PDF 115.2 KB Church gRNA design and cloning for Cas9 orthologs...optimized Cas9 PDF 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo...Fly: gRNA cloning pAc-sgRNA-Cas9 PDF 165.1 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF ...Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF 125.9 KB Sontheimer gRNA design and cloning All-in-one... helps design (10 different prediction scores), clone (primer design), and evaluate gRNAs, as well as ...for CRISPR design, homology directed repair and cloning . CRISPR Discussion Group (moderated by Feng Zhang...plasmids PDF 104.1 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; ...

...Additional Cloning Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol...provided as empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion...Protocol 237.2 KB Dual guide cloning: Brzezinski Lab Dual Guide Protocol 967.2 KB Plasmid and Sequence Derivative...

... of interest are cloned into a Firefly luciferase reporter vector via Gateway cloning to test for the ...Renilla and Firefly luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot...collection of empty backbone plasmids into which you can clone your regulatory element or gene of interest into... of 5' promoter/enhancer regions using Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc...Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc NanoLuc...Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB...Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly...

...Scott Lenaghan. Joint Modular Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional...as the promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Return to...Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent...Vladimir Nekrasov. The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized...plants (including rice and wheat) - Caixia Gao. Cloning The following collections are designed for creating... rescue in plants - Martin Parniske. GreenGate Cloning System to create plant expression vectors containing...constructs - Johannes Stuttmann. MoChlo: Modular Cloning Chloroplast Toolbox for design of synthetic operons...

...Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 KB SpCas9...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...annealed oligos (design is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold...annealed oligos (design is indicated below) can be cloned into the CRISPR array. The oligos are designed ...activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...for insertion of guide sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome...

... mutant clones from watermelon have been codon-optimized for expression in bacteria and cloned into an...region of MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available...malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research experience...IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of MDH and, if indicated on the...

...- Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning System - Geertsma...Parts Kit : Molecular cloning for E. coli - Densmore Lab EcoFlex MoClo : Modular cloning for applications ... and Dutzler Labs EasyClone 2.0 Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt...Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...

...well as by our trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies...synthetically produced recombinant antibodies, created by cloning antibody components into plasmids and expressing...Monoclonal antibodies are derived from an individual clone targeting a single antigen. An example of R-mAbs...and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following table highlights...mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies. Sci Rep... Rep. 2014 Jul 30;4:5885. Andrew Beavil Cloning and mammalian expression of mouse and human IgG monoclonals...

...Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly...Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification...

...336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one cloning step rather...Stephen Ekker Golden Gate TALEN 2.0 Golden Gate cloning method. Validated in multiple organisms. Dan Voytas...Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang... Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru...plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting in shorter...RR) used are obligate heterodimers that require cloning of left and right TALEN monomer proteins into opposite...enhancers in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat arrays...

...to add the CACC and AAAC cloning overhang. When designing oligos for cloning my target sequence into a... discriminate between positive and negative clones. Clones with insertion will show only linearized plasmid...Should I add the PAM sequence to the oligo when cloning my target spacers into the PX330 vector backbone...together, with each plasmid having a targeting spacer cloned into the backbone ( pX330 or PX335 , depending ... to target two genomic loci with wildtype Cas9, clone two spacers for each locus into PX330, then co-transfect...concern, you could go ahead with PX330 plasmid, clone in your target guides, test run them and then select... the Bench FAQs How can I quickly check if the cloning of my oligo into a backbone like PX330 worked? ...

...resistances. Comprehensive multi-cloning site compatible with standard cloning using many different restriction...Tn7 Transposition Nancy Craig A gene of interest cloned into one of these plasmids can be transposed into.... pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally His-tagged TALEN.... pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally His-tagged TALEN...His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 Tac (lactose/IPTG inducible...formation or prevent toxicity. Compatible with Gateway cloning. pGTvL1-SGC 39188 pTac (lactose/IPTG inducible)... and TEV cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB...

... expression, Ligation Independent Cloning (LIC) Bacteria Lac, T7, araBAD p15TV-L - His-tagged... Plasmids - More LIC bacterial cloning vectors pTD plasmid ...GFP-tag fusions for transcription pCSDest - Easy cloning of your gene using Gateway recombination... GFP for mammalian expression pcDNA3 GFP LIC cloning vector - C-terminal GFP for mammalian...Retroviral gene expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression... - Mammalian retroviral Gateway cloning vectors Adenoviral High transduction efficiency...RNAi For gene silencing experiments pLKO.1 - TRC cloning vector - Lentiviral plasmid for...

... PI 10878 pLKO.1 ‐ TRC cloning vector shRNA expression; Recommended for cloning new shRNAs (contains a...Below, browse our selection of empty vectors for cloning in shRNAs. Plasmids for constitutive expression...cassette from another vector (e.g. pSUPER) can be cloned into this vector; Contains a 5'LTR and should be...Read our detailed protocol for using the popular cloning vector pLKO.1. Trono Lab Lentivectors Toolbox Tyler...(CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...(CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick Aebischer and Didier ...1alpha promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...

...currently unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target... target sequence, as well as design and clone in the homology arms for the donor plasmid. The Mendenhall... also provided their protocol for homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB... complexes. This vector serves as a backbone to clone the left and right homology arms. More details on...al. Cell Rep. 2015 . Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1...include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt Unc-32::GFP targeting vector...

...promoter. WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express...well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors...pAdx-CMV-LacZ is uniquely suited to simplify the cloning process as it can be used in blue-white screening...Xgal/IPTG plates – if your insert is successfully cloned into this vector, transformants will instead form...

....1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748...shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono 11795...cassette (promoter and gene of interest) can be cloned into the plasmid. Nolan 12257 pWPXL 2nd EF-1alpha... the gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...