...cre/NLS Ple10 AVP pEMS1239 EGFP/NLS Ple11 AVP pEMS1240 EGFP/NLS Ple12 AVP pEMS1241 EGFP/NLS Ple12 AVP pEMS1482...EGFP/NLS Ple37 CRH pEMS1154 EGFP/NLS Ple38 CRH pEMS1155 EGFP/NLS Ple39 CRH pEMS1156 EGFP/NLS Ple44 CX3CR1...cre/NLS Ple51 DBH pEMS1362 EGFP/NLS Ple52 DCX pEMS1198 EGFP/NLS Ple53 DCX pEMS1199 EGFP/NLS Ple53 DCX pEMS1524...NLS Ple64 FEV pEMS1129 EGFP/cre/NLS Ple64 FEV pEMS1398 EGFP/NLS Ple66 FEV pEMS1131 EGFP/cre/NLS Ple66 FEV...NLS Ple93 GPR88 pEMS1161 EGFP/NLS Ple94 GPR88 pEMS1162 EGFP/NLS Ple95 GPR88 pEMS1163 EGFP/NLS Ple96 GPX3.../NLS Ple99 GRP pEMS1371 EGFP/NLS Ple100 GRP pEMS1372 EGFP/NLS Ple101 GRP pEMS1373 EGFP/NLS Ple102 GRP ...Plasmid Collections Pleiades Promoter Plasmids Pleiades Promoter Project The Pleiades Promoter Project (...

... Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection...

...antibody in assays with multiple targets, and is described in more detail in the Multiplex Assays section below...fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain fragment...differentiation between multiple antibodies. They are often used in assays that have multiple proteins to detect...application Collect and lyse samples to make proteins available. Tissue samples may need additional processing...proteins from a complex mix. Overview of application Process the tissue or cell culture samples to extract ...capture protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced...culture samples to extract proteins. Homogenize (tissues only) and lyse. Cross-link the samples with formaldehyde...

... C RISPR- as sociated c omplex for a ntiviral de fense; a complex of multiple Cas enzymes including Cas8...edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using dual...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single promoter...can be added to SHERLOCKv2 to detect multiple targets. For example, Cas12 can be added to detect the presence...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex to initiate DNA degradation. Cascade...have extended CRISPR applications to increasingly complex functions, including the ability to selectively...

...PLXNB2 plexin B2 KIAA0315, MM1, Nbla00445, PLEXB2, dJ402G11.3 PLXNB3 plexin B3 FLJ76953, PLEXB3, PLEXR... CD87, UPAR, URKR PLXNA1 plexin A1 NOV, NOVP, PLEXIN-A1, PLXN1 PLXNA2 plexin A2 FLJ11751, FLJ30634, KIAA0463...PLXN2 PLXNA3 plexin A3 6.3, HSSEXGENE, PLEXIN-A3, PLXN3, PLXN4, SEX, XAP-6 PLXNA4 plexin A4 DKFZp434G0625... KIAA1550, PLEXA4, PLXNA4A, PLXNA4B, PRO34003 PLXNB1 plexin B1 KIAA0407, MGC149167, PLEXIN-B1, PLXN5, ...PLXN6 PLXNC1 plexin C1 CD232, PLXN-C1, VESPR PLXND1 plexin D1 KIAA0620, MGC75353, PLEXD1 PPBP pro-platelet...

...Determine the reverse complement of each guide sequence. Example reverse complement sequences of the Pim1...Run samples on 2% agarose gel at 10 V/cm using 1x Tris-acetate-EDTA (TAE) buffer. Examine samples for ...322.2 KB If you have trouble loading this video, please try viewing on YouTube . Abstract The prokaryotic...of genes and genetic elements. Here we present a simple methodology for CRISPR design, cloning, and delivery...lines. Protocol For referenced Tables and Figures, please access the manuscript PDF posted at the top of ... of the mRNA transcript ( Figure 1B ). Use the example guides for the intended deletion of Pim1 in mouse...musculus ; Table 1 , Figure 2A ). NOTE: In this example, sgRNA-A’s protospacer sequence and PAM happen ...

...with CRISPR is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be easily expressed...nuclease form a complex with each individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out ...genome engineering, offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR...a protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy...out the DNA sequence complementary to the crRNA. In Type II CRISPR systems, a potential target sequence ...after where the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded DNA target...strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after the double-strand break. CRISPR systems...

...aureus Joung Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors... vitro transcription BsaI none S. pyogenes Mark pLenti-sgRNA-Lib 53121 Mammalian/Lentiviral BsmBI none...Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert pLenti SpBsmBI sgRNA Hygro 62205 Mammalian/Lentiviral ...Lentiviral BsmBI none S. pyogenes Hygro Maehr pLenti SpBsmBI sgRNA Puro 62207 Mammalian/Lentiviral BsmBI none S...62201 Plant yes, cut S. pyogenes Hyg Chen pUC57-Simple-gRNA backbone 51306 Xenopus BsaI none S. pyogenes...Bacteria BsaI yes, activate S. pyogenes Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian...71287 Plant U6-26p yes, cut S. pyogenes Hygro Chen pLenti-sgRNA 71409 Mammalian/Lentiviral hU6 none S. pyogenes...

...luciferase in plants Diego Orzaez 118069 MLRV Multiple Multiple Multi-luciferase reporter vector including...assess protein-protein interactions. NanoLuc® complementation : Protein-protein assays based on a split NanoLuc...more. nLuc/cLuc for split Firefly Luciferase complementation in plants. Bioluminescence Resonance Energy...Nano-lanterns) to allow for BRET-based in vivo imaging of multiple cellular processes, biosensors, or optogenetic...fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc...Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc...fusions using Gateway cloning Mikko Taipale 87074 pLenti6.2-ccdB-Firefly Firefly Creation of C-terminal Firefly...

... target populations defined by multiple parameters. Figure 1: Examples of intersectional cell population...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to...molecular payloads in target populations defined by multiple parameters. Depositor...Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References The development of powerful...Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to...definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations...order to enable three-feature cell targeting. C) Example of viral targeting of cells expressing Cre AND ...

...ion pore domain it is coupled to. Addgene has two main types of PSAMs: 5HT3 coupled, which are activating...the signaling pathway the engineered receptor is coupled to and the specific cell type where the receptor...most widely used chemogenetic receptors and are G-coupled protein receptors that have been engineered to ...hM4D(Gi) are all based on muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins, respectively. ... KORD is derived from the κ-opoid receptor and couples to the Gi G-protein. hM3D(Gq) and rM3D(Gs) are ...Selective Effector Molecules (PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ...activating, and GlyR-coupled, which are inhibitory. Another recently developed class of tools are Luminopsins...

...Looking For? Please let us know what you would like to see available to industry scientists. Please note this...we are allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer Information...Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric...account, you can place orders through our website. Please note that all orders must be placed online through... product pages after you login to your account. Please see our Help Center for more information on pricing...information. To learn more about Addgene’s MTA process please visit our Help Center . The MTA Process: Once your...Scientist Acknowledgement (RSA). This form can be completed electronically by the scientist who will be using...

...the following three buffers. All buffers are supplemented with 0.001% pluronic F68 or 0.014% Tween 20....available in each of the above serotypes and buffers. Please click on the plasmid name for ordering information...the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol...AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye, et al. ...AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite Ling, et al...

...experiments for multiplexed targeting with Zhang lab plasmids? For multiplexing CRISPR to target multiple genome...4 of the Cong, et. al, 2013 Science paper. For example, if you want to use double nickase, you could express...want to use wildtype cas9 or double nickase). For example, if you want to target two genomic loci with wildtype... vector, for one well in 24-well plate. A good example/reference is this Cell paper: http://www.cell.com...putting in the full target site in the donor. For example, choosing target sites that span the knock-in gene...selection marker to select transfected cells (for example, plasmid with a puromycin resistance cassette, ...individual genotyping (using Sanger sequencing, for example) on each colony in order to screen for positive...

...page . Note: For a list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog...for the 20 µL sized testing sample is denoted with a .T suffix (for example, AAV1.T). The catalog ID for...for the 100 µL sized sample does not have the suffix (for example, AAV1). AAV Vectors for Serotype Testing...different serotypes. In addition to the standard 100 µL sample size, the vectors listed below are also available...include virus associated DNA (vdna), which is a sample of the purified DNA from the original plasmid that...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...Cross lab collection for more) pLexA and pACT2.2 - Yeast 2-hybrid plasmids...understanding the function of your favorite gene. For example, fusing your protein to an epitope tag, such... Tet-inducible lentiviral shRNA expression pLenti CMV Neo DEST - Lentiviral Gateway...puro - Retroviral shRNA expression pLenti CMV Puro DEST - Lentiviral Gateway... Tet-inducible lentiviral shRNA expression pLenti CMV Hygro DEST - Lentiviral Gateway...destination vector for gene expression pLenti CMV/TO Hygro - Lentiviral Gateway...expression (tet inducible) Zeocin/Bleo Mammalian pLenti X1 GFP-Zeo DEST - Lentiviral Gateway...

...permitting selective simultaneous labeling in a sample. Please see the note below for information on substrates...Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England Biolabs offers non-toxic...community. Key features: Allows for visualization of multiple proteins simultaneously with dual labeling when...proteins Single constructs are compatible with multiple applications (different fluorophores, biotin, ...website. For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag...allow for dual labeling and visualization of complementary protein function or localization. ACP and MCP...MCP-tag Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and CLIP...

... kinase, is found in two complexes, mTORC1 and mTORC2. Each complex contains a unique subunit...p53 also promotes aberrant mTORC1 activity, and multiple familial cancers are caused by inactivating mutations...for the gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each...available plasmids for the gene. For components with multiple isoforms or subunits, individual links to each...each gene page are provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor... RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras homolog enriched in brain S6K Also known...Hippel-Lindau tumor suppressor Return to top mTORC2 Complex Symbol Name AKT AKT1 AKT2 AKT3 v-akt murine thymoma...

...Davidson 54924 mApple-MAPTau-C-10 MAPT mApple CMV Parkinson's, FTD Michael Davidson 54925 mApple-MAPTau-N-10...Cookson 13318 pLenti6-DEST PINK1-V5 G309D PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-... Cookson 29412 pLenti6-DJ1-V5-E18N PARK7 V5 CMV Parkinson's Mark Cookson 29413 pLenti6-DJ1-V5-C106D PARK7...Cookson 29414 pLenti6-DJ1-V5-L166P PARK7 His, V5 CMV Parkinson's Mark Cookson 29415 pLenti6-DJ1-V5-E18D ... Cookson 29417 pLenti6-DJ1-V5-E18Q PARK7 V5 CMV Parkinson's Mark Cookson 29418 pLenti6-DJ1-V5-D149A PARK7...ALS Mohan Babu 83444 pLEX_307-SOD1WT SOD1 EF-1 alpha ALS Mohan Babu 83445 pLEX_307-SOD1E133delta SOD1 EF...Mohan Babu 83446 pLEX_307-SOD1E133insTT SOD1 EF-1 alpha ALS Mohan Babu 83447 pLEX_307-SOD1E133K SOD1 EF...

...depdent on Cre, Flp, or Vcre Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of...mitochondria Mitycam, or ER targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for...indicators based on mRuby (jRCaMP1a, jRCaMP1b) and mApple (jRGECO1a) (Constitutive or Cre-dependent) Sensitive... Cre- or Flp-dependent) Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of...Cyan-Yellow Zinc FRET sensors ZapCV2 and ZapCV5 Droplet Microfluidic Flow Cytometer For Sorting On Transient...sensors for simultaneous monitoring of Zn(2)(+) in multiple cellular locations. PLoS One. 2012;7(11):e49371...Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities. Nat Cell ...