...plasmids for metabolic enzymes and pathways.... Plasmid Collections Synthetic Biology Metabolism Synthetic ...Synthetic Biology: Metabolism SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome...Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains...collection of synthetic biology plasmids related to metabolic pathways and components. Examples Include: Biofuels...Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table by keyword or sort ...

...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...protein of interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform...basic protocols and controls follow that of IF (above). Sample preparation, signaling excitation, and ...

...information about the type of genetic modification and target species. Additional information about each library...Activation Human Zhang 3rd 10 96,458 Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini...Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human Olzmann 3rd ~10...Yusa 3rd 5 90,230 Mouse Lipid and Sphingolipid Metabolism Knockout Libraries 206248 206249 Knockout Mouse...Mouse Birsoy 3rd 7–10 2,105 630 Mouse CRISPR Metabolic Gene Knockout Library 160129 Knockout Mouse Birsoy...Inhibition Yeast Tavazoie N/A 6-12 51,681 Mouse Metabolism Library 163966 Knockout Mouse Kimmelman 3rd ~...Knockout Mouse X.S. Liu 3rd 5 5 10 49,252 Human Metabolic Gene CRISPRa sgRNA Library 187080 Activation Human...

...the Pertz laboratory to construct custom biosensors. The ScEnSor Kit from the Olsson laboratory includes...sensors for live-cell imaging Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP ...protein biosensor for maltose Rapid construction of metabolite biosensors using domain-insertion profiling. ...David Savage Maltose Monitor maltose levels and metabolism (FLIPmal) Fluorescence Resonance Energy Transfer...Biosensing Reveals the Dynamics of Cytosolic Redox Metabolism in Plants. Plant Cell. 2020 Aug 13. pii: tpc.20.00241...concentration Semisynthetic sensor proteins enable metabolic assays at the point of care. Science. 2018 Sep...biosensor for trehalose Rapid construction of metabolite biosensors using domain-insertion profiling. ...

...Addgene's collection of KLF plasmids and learn more about the scientists who have created and shared these...Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About The Krüppel-like factors (KLFs) are a family of ...proliferation, differentiation, inflammation, metabolism, fibrosis, atherosclerosis, and carcinogenesis...transcriptional activation and/or repression. Continuing collaborative efforts within the KLF community have lead to...to contribute to this community resource. Learn about the benefits of depositing plasmids with Addgene...

...integrating MDH-related research into their teaching laboratories. They encourage instructors teaching different...research community and facilitates scientific collaborations between institutions. With these plasmids, ...regulation and post-translational modifications, and metabolic pathways and protein interactions. Plasmids A ...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and more....2021.43 (Link opens in a new window) External Collaboration Results in Student Learning Gains and Positive...

...reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components...Learn more about synthetic biology resources available at Addgene. Browse featured collections or depositing...Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas...Schmidt-Dannert Pamela Silver Lei Stanley Qi Jeff Tabor Jan-Willem Veening Christopher Voigt Clifford Wang...

... the cell and regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein...mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many...ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation...energy-intensive processes. mTORC1 also blocks catabolic processes including autophagy and lysosome biogenesis...

...in vitro ? These, in addition to many questions about bacteria themselves, can be answered using model...overexpressing or knocking down a gene can tell you much about its function and/or alter cellular activity in a...basic biology of a bacterial gene or redirecting metabolic pathways to produce a therapeutic compound, there...once you get it right, you can learn many things about your protein’s function, structure, and interactions...molecules, light, temperature, or even intracellular metabolite levels. Different plasmids and, often times, ...pCph8 50552 pLtetO-1 Anhydrotetracycline Jeffrey Tabor Anhydrotetracycline inducible expression of red ...

...spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert Pan and Alex Schier Labs. Multicolor ...zebrafish strains and materials and information about zebrafish research. China Zebrafish Resource Center...America. The Zebrafish Book - A guide for the laboratory use of zebrafish. CRISPRz - A database of CRISPR...gene expression database that provides information about when genes are active in developing embryos. zfRegeneration...

... Frederick National Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research...

...resource is a collaboration between the Cell Migration Consortium and Addgene. Member laboratories of the Cell...This resource is a collaboration between the Cell Migration Consortium (CMC) and Addgene, which highlights...

... information about: γ-Retroviral packaging systems Frequently asked questions (FAQ) about retrovirus Popular...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...

...Learn about Addgene's 2018 AAV Education and Development (AAVED) workshop series.... Institute Katherine Matho Cold Spring Harbor Laboratory Gowan Tervo Janelia Research Campus Sarada Viswanathan...Institute Katherine Matho, Cold Spring Harbor Laboratory Gowan Tervo and Sarada Viswanathan, Janelia Research...

...Virus Should I Use? Use this guide to learn more about the different types of viruses that are commonly...temporary in the host. Use this guide to learn more about the different types of viruses that are commonly...found in the guides for each virus type, linked above. Virus Expression Genome Packaging Capacity Virus...

... Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd generation...systems Viral production Frequently asked questions about lentivirus use Glossary of commonly used lentiviral... or shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono...

...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using CRISPR...with support from the BRAIN Initiative. Jackson Laboratory (Link opens in a new window) A collection of ...

... working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid resource...contact information by following the link to their laboratory website. Mouse Line Mutation (DNA) Background...contact information by following the link to their laboratory website. Cell Line Mutation (DNA) Mutation (protein...

...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable...when carrying out CRISPR experiments. Read more about how to design your gRNA . Browse Plasmids: gRNAs...to optimize your delivery conditions. Learn more about CRISPR delivery in mammalian systems . Validate ...

...Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids from 2016 blog post...circularly permuted Venus fluorophore. Learn more about pHlash sensor in our 28 Hot Plasmid Technologies... with the original STARR-seq method. Learn more about STARR-seq in our 27 Hot Plasmids from 2016 blog ...