...cre/NLS Ple10 AVP pEMS1239 EGFP/NLS Ple11 AVP pEMS1240 EGFP/NLS Ple12 AVP pEMS1241 EGFP/NLS Ple12 AVP pEMS1482...EGFP/NLS Ple37 CRH pEMS1154 EGFP/NLS Ple38 CRH pEMS1155 EGFP/NLS Ple39 CRH pEMS1156 EGFP/NLS Ple44 CX3CR1...cre/NLS Ple51 DBH pEMS1362 EGFP/NLS Ple52 DCX pEMS1198 EGFP/NLS Ple53 DCX pEMS1199 EGFP/NLS Ple53 DCX pEMS1524...NLS Ple64 FEV pEMS1129 EGFP/cre/NLS Ple64 FEV pEMS1398 EGFP/NLS Ple66 FEV pEMS1131 EGFP/cre/NLS Ple66 FEV...NLS Ple93 GPR88 pEMS1161 EGFP/NLS Ple94 GPR88 pEMS1162 EGFP/NLS Ple95 GPR88 pEMS1163 EGFP/NLS Ple96 GPX3.../NLS Ple99 GRP pEMS1371 EGFP/NLS Ple100 GRP pEMS1372 EGFP/NLS Ple101 GRP pEMS1373 EGFP/NLS Ple102 GRP ...Plasmid Collections Pleiades Promoter Plasmids Pleiades Promoter Project The Pleiades Promoter Project (...

... Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection...

...antibody in assays with multiple targets, and is described in more detail in the Multiplex Assays section below...fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain fragment...differentiation between multiple antibodies. They are often used in assays that have multiple proteins to detect...application Collect and lyse samples to make proteins available. Tissue samples may need additional processing...proteins from a complex mix. Overview of application Process the tissue or cell culture samples to extract ...capture protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced...culture samples to extract proteins. Homogenize (tissues only) and lyse. Cross-link the samples with formaldehyde...

... C RISPR- as sociated c omplex for a ntiviral de fense; a complex of multiple Cas enzymes including Cas8...edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using dual...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single promoter...can be added to SHERLOCKv2 to detect multiple targets. For example, Cas12 can be added to detect the presence...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex to initiate DNA degradation. Cascade...have extended CRISPR applications to increasingly complex functions, including the ability to selectively...

...PLXNB2 plexin B2 KIAA0315, MM1, Nbla00445, PLEXB2, dJ402G11.3 PLXNB3 plexin B3 FLJ76953, PLEXB3, PLEXR... CD87, UPAR, URKR PLXNA1 plexin A1 NOV, NOVP, PLEXIN-A1, PLXN1 PLXNA2 plexin A2 FLJ11751, FLJ30634, KIAA0463...PLXN2 PLXNA3 plexin A3 6.3, HSSEXGENE, PLEXIN-A3, PLXN3, PLXN4, SEX, XAP-6 PLXNA4 plexin A4 DKFZp434G0625... KIAA1550, PLEXA4, PLXNA4A, PLXNA4B, PRO34003 PLXNB1 plexin B1 KIAA0407, MGC149167, PLEXIN-B1, PLXN5, ...PLXN6 PLXNC1 plexin C1 CD232, PLXN-C1, VESPR PLXND1 plexin D1 KIAA0620, MGC75353, PLEXD1 PPBP pro-platelet...

...Determine the reverse complement of each guide sequence. Example reverse complement sequences of the Pim1...Run samples on 2% agarose gel at 10 V/cm using 1x Tris-acetate-EDTA (TAE) buffer. Examine samples for ...322.2 KB If you have trouble loading this video, please try viewing on YouTube . Abstract The prokaryotic...of genes and genetic elements. Here we present a simple methodology for CRISPR design, cloning, and delivery...lines. Protocol For referenced Tables and Figures, please access the manuscript PDF posted at the top of ... of the mRNA transcript ( Figure 1B ). Use the example guides for the intended deletion of Pim1 in mouse...musculus ; Table 1 , Figure 2A ). NOTE: In this example, sgRNA-A’s protospacer sequence and PAM happen ...

...with CRISPR is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be easily expressed...nuclease form a complex with each individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out ...genome engineering, offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR...a protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy...out the DNA sequence complementary to the crRNA. In Type II CRISPR systems, a potential target sequence ...after where the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded DNA target...strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after the double-strand break. CRISPR systems...

...aureus Joung Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors... vitro transcription BsaI none S. pyogenes Mark pLenti-sgRNA-Lib 53121 Mammalian/Lentiviral BsmBI none...Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert pLenti SpBsmBI sgRNA Hygro 62205 Mammalian/Lentiviral ...Lentiviral BsmBI none S. pyogenes Hygro Maehr pLenti SpBsmBI sgRNA Puro 62207 Mammalian/Lentiviral BsmBI none S...62201 Plant yes, cut S. pyogenes Hyg Chen pUC57-Simple-gRNA backbone 51306 Xenopus BsaI none S. pyogenes...Bacteria BsaI yes, activate S. pyogenes Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian...71287 Plant U6-26p yes, cut S. pyogenes Hygro Chen pLenti-sgRNA 71409 Mammalian/Lentiviral hU6 none S. pyogenes...

...luciferase in plants Diego Orzaez 118069 MLRV Multiple Multiple Multi-luciferase reporter vector including...assess protein-protein interactions. NanoLuc® complementation : Protein-protein assays based on a split NanoLuc...more. nLuc/cLuc for split Firefly Luciferase complementation in plants. Bioluminescence Resonance Energy...Nano-lanterns) to allow for BRET-based in vivo imaging of multiple cellular processes, biosensors, or optogenetic...fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc...Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc...fusions using Gateway cloning Mikko Taipale 87074 pLenti6.2-ccdB-Firefly Firefly Creation of C-terminal Firefly...

... target populations defined by multiple parameters. Figure 1: Examples of intersectional cell population...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to...molecular payloads in target populations defined by multiple parameters. Depositor...Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References The development of powerful...Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to...definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations...order to enable three-feature cell targeting. C) Example of viral targeting of cells expressing Cre AND ...