Plasmids 101: Yeast Vectors
Type
Blog Post
Published
Feb. 25, 2014, 7:11 p.m.
...previously described.
Why do scientists use yeast vectors?
Yeast are eukaryotes and thus contain complex ...between E. coli and yeast cells. To be useful in the lab, the vectors must contain a yeast-specific origin...selection in yeast cells, in addition to the bacterial ORI and antibiotic selection markers.
The yeast origin...within a yeast vector determine how the plasmid is replicated and maintained within the yeast cell. These...episome. The four main types of yeast plasmids are defined below:
Yeast Integrating plasmids (YIp): These...the yeast chromosome. As the name suggests, these vectors can replicate independently of the yeast chromosome...extensive list of yeast auxotrophic markers and includes the associated references.
Yeast selection marker...