Plasmids 101: Using Transposons in the Lab
Type
Blog Post
Published
July 6, 2021, 1:28 p.m.
...eukaryotic genomes.
Components of a transposon system
Transposon: The transposon is the DNA sequence that moves...
piggyBac plasmids
Tol2
Tol2 was the first reported active DNA transposon in vertebrates. It was ...transposition activity
Tol2 transposon plasmids
Applications of transposons
Now that you’ve learned...also called retrotransposons, and Class II TEs, also called DNA transposons. DNA transposons are commonly...Sleeping Beauty. Minimal Tol2 or miniTol2 is a truncated version of the original Tol2 and has ~3-fold increase...discovered transposons due to changes in coloration of corn kernels. Aside from corn, transposons are in ...2010).
So why do transposons matter? The outcome really depends on where a transposon lands. For example...