Live and Let Dye: Self-Labeling Protein Tags
Type
Blog Post
Published
April 16, 2024, 1:15 p.m.
...a look at some popular self-labeling tools:
Tetracysteine: The first reported chemical labeling tag, from...Commercial ligands available
Ease of use
Tetracysteine (Cys4)
15
Biarsenical dyes (FlAsH, ReAsH...labeling specificity than short peptide tags like tetracysteine (Jing & Cornish, 2011). HaloTag and SNAP-tag...functional. In some cases, a small peptide tag like tetracysteine might be the only option that doesn’t disrupt...options for CLIP-tag and very few TMP-tag or tetracysteine labeling dyes.
If reaction speed is a priority...