...is the tetracycline (Tet)-regulated system. You can choose either the Tet-off system or the Tet-on system... interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3) and one with either tTA or rtTA (FUW-M2rtTA). In this kind...system. In the Tet-off system a Tet-response element (TRE) is placed upstream of the promoter in your transfer...expression. In contrast, in the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence...expression kinetics as compared to the Tet-off system. One drawback of the Tet system is that it often requires... In the absence of tetracycline or a derivative such as Doxycycline (Dox), the Tet-controlled transactivator...vector systems containing both your transgene and tTA/rtTA (pINDUCER20, pINDUCER21) have been developed, but...

...lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve as controls...experiments, while rtTA can be used to activate expression of a gene under the control of the tet operator. AAV...

...inducible promoters. The positive inducible tetracycline ON (Tet-On) system, a versatile tool developed for...activation. In this system, the activator rtTA (reverse tetracycline-controlled transactivator) is normally...Addgene.org: Inducible Promoters for Bacteria Tetracycline (Tet) On Expression Systems at Addgene Cre-lox ...lot. If you’re looking for high inducibility, the Tet-On system may be a good choice, as it’s documented...require only the addition of an external inducer, like Tet systems, may be activated very rapidly. In contrast...and cannot bind the tetracycline response elements (TRE) in a promoter. Tetracycline and its derivatives...

...transposase. In rtTA-cell lines, it is better to first engineer the cell line with rtTA, then integrate...directly in transient transfection in rtTA+ cell lines (such as HEK293-TETOn cells), or they can also be integrated...

...recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA or rtTA) are between 1 to 2 kb in ...

...has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to show its usefulness...cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were pulsed with 2µg/ml of Doxycycline...repressor plasmids from Scot Wolfe's lab. These include Tet-inducible CRISPR activators and repressor plasmids...