...change that placesTagRFP-T and Dronpa in close proximity. By monitoring changes in TagRFP-T fluorescence...Angela Holmes Jin Zhang’s lab is developing new super-resolution techniques for live-cell imaging of the...FLINC)-based biosensors measure fluctuations of TagRFP-T fluorescence intensity due to interactions with...membrane in living cells using a construct in which TagRFP-T and Dronpa flanked a PKA substrate fused to the...

...example, superfolder GFP (sfGFP) and mNeonGFP can fold in <10min at 37°C, mCherry takes ~15min, TagRFP ~100min...is set as 1. Some proteins are very dim (e.g. TagRFP657, which has a brightness of 0.1) and this should...

...oligomers such as DsRed or high affinity FPs such as TagRFP. Problem 3 can be solved, in most cases, by using...monomeric FPs. EGFP and other GFP family members (superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise,...

...emission spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors...set out to “rule” Molecular Biology. Under the supervision of Dr. Tan, a team of undergraduates created ...