...single-stranded AAV (ssAAV) and a self-complementary AAV (scAAV) vector. Full-length ssAAV vector genome of both...annealing or second strand synthesis. In contrast, a scAAV vector with half the size of the ssAAV genome has...annealing. Figure and legend from Okada, 2013.   scAAV If your gene of interest is truly small (<2.5Kb)... expression in a hurry, self-complementary AAV (scAAV) may be an option (McCarty et al., 2001). This AAV...Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently...

...AAV constructs with self-complementing structure (scAAV) (7). As single stranded DNA viruses, AAVs normally...strand synthesis before gene expression begins. ScAAV avoid this rate limiting step and can immediately...undergo replication and transcription. However, with scAAV the effective genome size usable is shortened (from...Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently...

...packaging the virus as self-complementary AAV (scAAV). However, scAAV vectors have limited size compared to single-strand...