...TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, reverse...Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC...provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes.... Find lentiGuide-Puro. pSpCas9n(BB)-2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n...best to go with pSpCas9(BB)-2A-Puro (PX459) V2.0. Find pSpCas9(BB)-2A-Puro (PX459). Thank you! We would... experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David...in the puromycin cassette that makes it less efficient in some cell lines. pSpCas9(BB)-2A-Puro (PX459)...

...backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids...Plasmids -  If you're familiar with the pLKO plasmids you'll note that those are lentiviral constructs. In...negative control, and empty backbones such as pBABE-puro for retroviral expression and pLVTHM, a lentiviral...

...protocol on how to use the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid...GFP expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated...Vectors    Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest...here. Cloning and expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by ...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...

...vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). Check out our complete guide...and carry the metallothionein promoter (e.g. pMT-puro). Worm vectors There are several approaches to studying...