...plasmids with the piggyBac™ transposon. These plasmids, when combined with a source of piggyBac™ transposase...genome. We encourage you to deposit your piggyBac™ transposon vectors with us to help us expand this useful...Goodwin from Transposagen What is the piggyBac™ transposon? The piggyBac™ transposon was originally identified... transposons, piggyBac™ has two components, a transposon and a transposase. The piggyBac™ transposase ...excision-only piggyBac™ transposase. Image courtesy of Transposagen.   How does piggyBac™ enhance gene...technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon ...removed using the piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in...

...Sleeping Beauty plasmids  piggyBac Although its name suggests otherwise, piggyBac was discovered in the cabbage... Beauty, piggyBac has an integration efficiency in human cells similar to retroviral vectors. The hyperactive...compared to the codon-optimized wild-type piggyBac transposase.  piggyBac plasmids    Tol2  Tol2 was the first...with Sleeping Beauty and piggyBac, which is a big advantage over viral vectors (~5 kB payload for AAV and...in viral vectors, but easily fit in a TE. Transposons are also less likely than viral vectors to induce...transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three different ...adapted for use as research tools: Sleeping beauty, PiggyBac, and Tol2.  Sleeping beauty Sleeping Beauty is...

... Institute developed fluorescently labeled piggyBac vectors for uniform and sustained expression of single...both dCas9 and gRNAs. The multi-gRNA piggyBac vector integrates into genomic DNA with the help of...pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly used for in vivo biotinylation via BirA... by a doxycycline inducible promoter. The gRNA piggyBac plasmids include a mRFP reporter. With this system...transcript and protein levels. Plasmids for multiplexed piggyBac gRNA delivery and the all-in-one dCas9-KRAB and...found at Addgene. Check out these plasmids for piggyBac gRNA delivery and the all-in-one dCas9 targeting...

...’s lab developed new fluorescently labeled piggyBac vectors to deliver uniform and sustained expression... Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system for human pluripotent stem cells...

...residual expression piggyBac Excisible Medium Medium Generates transgene-free and vector-free cells Genome...so either a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming...reprogramming vectors available from Addgene. Another drawback is that lentiviral vectors insert in different...Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...Non-integrating iPSC delivery methods Viral Vectors Adenovirus: Adenoviral vectors infect dividing and nondividing...reprogramming factors: Mouse Expression Vectors Human Expression Vectors Carey et al Hockemeyer et al Brambrink...Non-integrating Low Medium Generates transgene-free and vector-free cells No genomic integration Inefficient...

...out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9 SAM vector! On/off control all the time If ...knock out plasmids? Check out our Cre vector and Murine LoxP vector.   Turn it off for a little while Transient...? Check out our Retro expression vector and Lenti expression vector. Turn it on for just a little while...Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging vector! Destabilizing domains Destabilizing...Lentiviral Tet-on and AAVS1 targeted Tet-on transgene vector. Degron tags Tagging relevant proteins with a degron...levels. Looking for a DD? Check out our DD-Cas9 vector! The price of tunable control These systems can...

...modular vector assembly process designed for a quick, plug-and-play approach to cloning CRISPR vectors (McGee...for each module, generate the vector file, and quickly clone their vector of interest. And it truly is ...destination vectors for various delivery methods, along with compatible inserts. All destination vectors contain...heavy lifting out of CRISPR vector design, making it easier to get the vectors that are just right for your...Fragmid destination vectors currently available. Table 1: Fragmid destination vectors Organism Delivery...designing a CRISPR vector the Fragmid way!    Figure 1: Selecting your vector type on the Fragmid...plasmid and generating the vector file on the Fragmid website.   If the vector is to your liking, your...

...deliver in low-capacity vectors like AAV. AAV (adeno-associated viral vectors) are commonly used for in...CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors in Genome Editing Resources on Addgene.org...preferential infection of certain tissues. However, these vectors have a low genetic capacity of ~4.5 kb. At 4.1 ...previously used SaCas9 (1053 aa/3.2 kb) with AAV vectors for efficient postnatal editing. LbCpf1 and AsCpf1...SpCas9. These nucleases should fit well into AAV vectors, especially given that Cpf1 gRNAs are also shorter...

...reprogramming discrepancies. The polycistronic piggyBac transposon vectors tested in this work are available at ...TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that have standard cloning sites or gateway compatible...neuronal tools to the expanding gateway compatible vectors currently shared amongst the zebrafish community...lead to comparable experimental results. Using PiggyBac transposons, the Woltjen Lab compared different...for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons here. Dimidschstein...Very low off-target activity; protein purification vector  87439  pCMV-HF-BE3  HF-Cas9 (NGG)  Very low ...low off-target activity; mammalian expression vector Rees H, et al. Nature Communications. 2017. PubMed...

...expression vectors with various promoters suitable for in vivo expression, and/or produce vectors to make...prototrophic strains. The vectors were based on the original EasyClone vectors (Jensen et al 2013) allow...donor vectors containing mAID and either Neo or Hygro resistance markers with CRISPR/Cas vectors targeting...uses the piggybac transposase to integrate dCas9-VPR into the genome of target cells. The piggybac system... interest and cloned into provided destination vectors using low-cost, one-pot golden gate cloning that... Jacks Lab constructed several GMAP-compatible vectors for a wide variety of biological applications. ... problem, Csaba Pál’s lab has created a set of vectors that allows one to use the MAGE method in unmodified...