Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
Type
Blog Post
Published
June 27, 2018, 1:16 p.m.
...dSpyCas9-APEX2 fusion.
This Sontheimer lab used this technology, which they call C-BERST (dCas9–APEX2 biotinylation...tyrosine sulfation consensus sequence, fluorophore, or APEX2), allowing for quantitative analysis using different...catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed from a Tet/Shield1 inducible CMV promoter... cells are treated with biotin–phenol and H2O2, APEX2 labels electron-rich amino acid side chains (e.g...