... Systems (MuLE) Kit, the MultiSite Gateway cloning kit, and MultiSite Gateway plasmids. Constant reading... the Gateway cloning method and its advantages. An introduction to Gateway technology The Gateway cloning...one Gateway vector to produce the desired expression clone. This is possible thanks to the Gateway vectors...attR2, and so on. Take a look at some of the Gateway Multisite plasmids available at Addgene, including the...links below to find Gateway plasmids for your organisms of interest: Find Gateway cloning plasmids here... needs. Since its invention in the late 1990s, Gateway cloning technology has become very popular as a...appropriate entry and destination vectors, one can use Gateway to clone a gene of interest into a variety of expression...

...based on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a customizable...model systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry clones to be...with a heterologous promoter outside of the Multisite Gateway® cassette, you can specifically express your...that are combined in a specified order using Multisite Gateway cloning. A desired insert is cloned using ... combined with custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector... phage integrase system which is the basis for Gateway cloning. The combination of all 11 att pairs in...customizable Gateway®-based toolkit that allows scientists to generate fusion proteins from any ORF available...

...combining the advantages of Golden Gate and Multisite Gateway cloning methods developed by the lab of Joachim...Finally, the destination vectors are used with Multisite Gateway cloning to generate the final construct. ... eight entry vectors can be used for each Multisite Gateway compatible destination vector for a maximum...component plasmids from building blocks via Golden GATEway Cloning Frustrated by the complexity of assembling...just the set of tools that you need. The Golden GATEway cloning kit simplifies the cloning process for ...transgenesis construct. Plasmids constructed using Golden GATEway cloning have been utilized to create recombination...