...-BY license.   Find MTRIA sensors here!   Ino D, et al. A fluorescent sensor for real-time measurement...interest, they create sensors for FGFR1, EGFR and ErbB2. And orthogonal sensors with different ITAM/SH2... new viral preps of the dopamine sensor dLight1.2 and voltage sensor Ace2N and additional GCaMP6 constructs...hottest fluorescent biosensors new to Addgene this year. Feel the love with MTRIA sensors One of the latest... class of sensors for neuropeptides and neurotransmitters. Motivated to develop a biosensor for oxytocin...engineering improved sensors for these and other targets. Figure 1: MTRIA biosensors are generated by inserting... potassium sensors Whatever your analyte of interest, it’s important to choose a biosensor that has a ...

...2019, the first generation of norepinephrine sensors was developed, named GRABNE, which helped researchers...from Kagiampaki et al., 2023. nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG...GRABNE is blocked by alpha2-AR antagonist, nLight sensors are not. Instead, they are blocked by alpha1-AR...advantages of this next generation of norepinephrine sensors is their speed. nLightG is activated eight times...selectivity for norepinephrine over dopamine. All three sensors, however, did display some reaction to high concentrations...Li, Y. (2019). A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine...brain but have been difficult to study without biosensors to directly visualize their activity. In 2019...

...in the 3’ UTR by four miRNA target sites and thereby serves as a sensor of miRNA presence and activity...field further with the creation of the largest miRNA sensor collection reported to date, containing all ...PubMed PMID: 29300010. miRNA reporter vectors shed new light on mechanisms of miRNA-mediated gene regulation...Listen to the miRNA reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized... a subset of active miRNA’s unique to HEK293FT and HeLa cell lines. When the miRNA activity profile for...Despite several years of research, the mechanisms of miRNA regulation have yet to be fully elucidated. Ron ...all 620 sequences of probable mature human miRNAs. Weiss’s collection consists of a library of reporter...

...research New viral vector preps of Voltron2 voltage sensors AAV vectors for targeting microglia The REVeRT ....2021.01.003. PMID: 33714753. Voltron2 voltage sensors By Brian O’Neill New viral vector preps expressing...neurons in vivo. Because they are negative-going sensors (with fluorescent signal at baseline voltage), ... specificity of ssAAV, a targeting sequence of miR124T was inserted into the vector to silence transgene...AAV vectors, either alone or in combination with miR124T targeting sequences, provides a simple yet valuable...

...-6. doi: 10.1038/nmeth.3147. Bioluminescent pH sensor Understanding intracellular pH regulation is important...events. To overcome the issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters... biology tools by engineering light-switchable sensors developed from bacterial two-component signal transduction...transcription activation or repression. The TCS light sensors described in ACS Synthetic Biology (2014) represent... and 4) optimizing expression of both the light sensor and the response regulator. These changes reduced...expression, shRNA/shRNA-miR30 constitutive (H1, U6, 7SK) or inducible shRNA-miR30 (CMV-TO) expression, or...or inducible, gene knockdown with shRNA or shRNA-miR30, (2) tamoxifen-inducible CreERT2 gene deletion, ...

...engineered ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category...effectively deploying them in the lab somewhat mirrors the complexity of understanding their wild counterparts...large percentage of viral vector preps contain biosensors, optogenetic tools, and chemogenetic tools: protein... most commonly requested (Figure 3). Over 400 biosensors were distributed in 2023 alone. You’ll also find...in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization) was first developed...

...transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting...Gene regulatory elements [promoters and enhancers, miRNA target sites, and recombination target sequences...expression to your cell type of interest, while miRNA target sites can be used to prevent expression in... chemogenetic tools. Dual expression Promoters, miRNAs, enhancers, and switches all take up precious cargo...immune response against the capsids or transgenes. Sensory neurons and liver appear especially susceptible...PNS, including ENS (broad): somatic and visceral sensory ganglia, type II spiral ganglion neurons Mice...Chaudhari, N. (2022). Selectively Imaging Cranial Sensory Ganglion Neurons Using AAV-PHP.S. eNeuro,9(3), ...

.... FRET biosensors were developed based on miRFP760-miRFP720 FRET pair, including Rac1 biosensor (Shcherbakova...Enhanced miRFPs are monomeric and bright Recent series of enhanced monomeric NIR FPs named miRFPs (monomeric...Therefore, we recommend using miRFPs and their enhanced versions named emiRFPs in your applications.  There...set of spectrally distinct (e)miRFPs available. The numbers in (e)miRFP names correspond to the maxima...multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from cyanobacteriochrome...advantages are their small size (17 kDa for miRFPnanos, 35 kDa for miRFPs, and 27 kDa for EGFP) and a possibility... miRFP703 https://www.addgene.org/79988/ 674 703 90,900 8.6 127 Monomer 394 4.5 37 miRFP709 https...

...Vladislav Verkhusha iRFP GAF domains from miRFP709 and miRFP670, pair with common PAS domain. Requires presence...blog Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors Collection Split ...near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Shcherbakova et al. Nat...

...Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson...well as Ligand-Independent Functional Changes in Sensory Neurons. The Journal of Neuroscience, 36(42), 10769...