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Showing: 1 - 20 of 125 results

  1. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ... PubMed PMID: 25114216. PubMed Central PMCID: PMC4151723. 2. Doench, John G., et al. "Optimized sgRNA ... PubMed PMID: 26780180. PubMed Central PMCID: PMC4744125. 3. Gilbert, Luke A., et al. "Genome-scale CRISPR-mediated... PubMed PMID: 25307932. PubMed Central PMCID: PMC4253859. 4. Hart, Traver, et al. "High-Resolution CRISPR... PubMed PMID: 25494202. PubMed Central PMCID: PMC4420636. 7. Ma, Hongming, et al. "A CRISPR-based screen... PubMed PMID: 26190106. PubMed Central PMCID: PMC4559080. 8. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9... PubMed PMID: 24336571. PubMed Central PMCID: PMC4089965. 9. Wang, Tim, et al. "Genetic screens in human... PubMed PMID: 26280411. PubMed Central PMCID: PMC4785103. 11. Wong, Alan SL, et al. "Multiplexed barcoded...

  2. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... PubMed PMID: 25337876. PubMed Central PMCID: PMC4270925. Find plasmids from this paper at Addgene. ... PubMed PMID: 25122746. PubMed Central PMCID: PMC4231726. Find plasmids from this paper at Addgene. ... PubMed PMID: 24954249. PubMed Central PMCID: PMC4066266. Find plasmids from this paper at Addgene. ... PubMed PMID: 25751063. PubMed Central PMCID: PMC4396471. Find plasmids from this paper at Addgene. ... PubMed PMID: 24770325. PubMed Central PMCID: PMC4090141. Find plasmids from this paper at Addgene. ... PubMed PMID: 25432517. PubMed Central PMCID: PMC4262988. Find plasmids from this paper at Addgene. ... PubMed PMID: 25733849. PubMed Central PMCID: PMC4371917. Find plasmids from this paper at Addgene. ...

  3. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ... PubMed PMID: 24291815. PubMed Central PMCID: PMC4101921. 19. Dias, J., et al., Generation of red blood... PubMed PMID: 24930130. PubMed Central PMCID: PMC4169698. 24. Lian, X., et al., Robust cardiomyocyte differentiation... PubMed PMID: 26214132. PubMed Central PMCID: PMC4566857. 26. Maffioletti, S.M., et al., Efficient derivation... PubMed PMID: 25578634. PubMed Central PMCID: PMC4412587. 28. Mohsen-Kanson, T., et al., Differentiation... PubMed PMID: 24510784. PubMed Central PMCID: PMC4096605. 30. Lamba, D.A., et al., Generation, purification... PubMed PMID: 27013738. PubMed Central PMCID: PMC4835250. 32. Ma, L., Y. Liu, and S.C. Zhang, Directed... PubMed PMID: 25782665. PubMed Central PMCID: PMC4363833. 35. Eguizabal, C., et al., Dedifferentiation...

  4. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ... PubMed PMID: 24509632. PubMed Central PMCID: PMC4063274. 2. Findlay, S. D., Vincent, K. M., Berman, J... (2016). PubMed PMID: 27089539. PubMed PMCID: PMC4835065. 3. Mock, U., Hauber, I. & Fehse, B. Digital ... PubMed PMID: 25964300. PubMed Central PMCID: PMC4477672. 5. Sedlak, R. H. et al. Digital detection of... PubMed PMID: 26829887. PubMed Central PMCID: PMC4735761. 6. Fu, Y. et al. High-frequency off-target mutagenesis... PubMed PMID: 25566793. PubMed Central PMCID: PMC4349094.  ...

  5. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ... PubMed PMID: 25184501. PubMed Central PMCID: MC4262738. 2. Doench, John G., et al. "Optimized sgRNA design... PubMed PMID: 26780180. PubMed Central PMCID: PMC4744125.  3. Gilbert, Luke A., et al. "Genome-scale CRISPR-mediated... PubMed PMID: 25307932. PubMed Central PMCID: MC4253859.  4. Konermann, Silvana, et al. "Genome-scale .... PubMed PMID: 25494202. PubMed Centra PMCID: MC4420636.  5. Sanson, Kendall R., et al. "Up, down, and... PubMed PMID: 25307933. PubMed Central PMCID: PMC4252608.  Additional Resources on the Addgene Blog Read...

  6. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... PubMed PMID: 25713381. PubMed Central PMCID: PMC4364232. 2. Baker, Monya. “RNA imaging in situ”. Nature... PubMed PMID: 26000751. PubMed Central PMCID: PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology... PubMed PMID: 25019067. PubMed Central PMCID: PMC4071757. 6. Hong, He, et al. “Molecular Imaging with ... PubMed PMID: 27446908. PubMed Central PMCID: PMC4923196. 8. Sassolas, Audrey, et al. “Optical detection... PubMed PMID: 26877022. PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based...

  7. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ... PubMed PMID: 25837586. PubMed Central PMCID: PMC4383620. Botman, Dennis, et al. "In vivo characterisation... PubMed PMID: 27240257. PubMed Central PMCID: PMC4927352. Janetopoulos, Chris, Tian Jin, and Peter Devreotes... PubMed PMID: 25875503. PubMed Central PMCID: PMC4397040. Lambert, Talley J. "FPbase: A community-editable... PubMed PMID: 15247428. PubMed Central PMCID: PMC490022. Shaner, Nathan C., et al. "Improving the photostability... PubMed PMID: 26799488. PubMed Central PMCID: PMC4723041. Additional resources on the Addgene blog Read...

  8. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... PubMed PMID: 25566793. PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput... PubMed PMID: 25409780. PubMed Central PMCID: PMC4246457. 4. Davidson CJ., et al. "Improving the limit... PubMed PMID: 25595557. PubMed Central PMCID: PMC4308725. 7. Pattanayak, Vikram, et al. "High-throughput... PubMed PMID: 25513782. PubMed Central PMCID: PMC4320685. 9. Kim, Daesik, et al. "Digenome-seq: genome-wide... PubMed PMID: 25503383. PubMed Central PMCID: PMC4320661. 14. Crosetto, Nicola, et al. "Nucleotide-resolution...

  9. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ... Pubmed PMID: 24733905. Pubmed Central PMCID: PMC4000813. 2. McDowell, Gary S., et al. "Shaping the Future... Pubmed PMID: 25653845. Pubmed Central PMCID: PMC4304227. 3. Sauermann, Henry, and Michael Roach. "Science... Pubmed PMID: 25925677. Pubmed Central PMCID: PMC4484856. 5. Bourne, Henry R. "A fair deal for PhD students... Pubmed PMID: 25291257. Pubmed Central PMCID: PMC4185419. 7. NIH 2012. Biomedical research workforce working...

  10. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...). PubMed: PMID 252409288. PubMed Central: PMCID PMC4˚237163. Find plasmids from this paper at Addgene...PubMed: PMID 25282355. PubMed Central: PMCID: PMC4317352. 3. Beisel, Chase L., Ahmed A. Gomaa, and Rodolphe... PubMed: PMID 25417800. PubMed Central: PMCID PMC4282009. 4. Yosef, Ido, Miriam Manor, Ruth Kiro, and ... PubMed: PMID 26060300. PubMed Central: PMCID PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. ...

  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... PubMed PMID: 25555356. PubMed Central PMCID: PMC4324159. Castle, M.J., Gershenson, Z.T., Giles, A.R.,... PubMed PMID: 24694006. PubMed Central PMCID: PMC4137353. Chan, K.Y., Jang, M.J., Yoo, B.B., Greenbaum... PubMed PMID: 26696844. PubMed Central PMCID: PMC4678200.  Kohara, K., Pignatelli, M., Rivest, A.J., Jung... PubMed PMID: 24336151. PubMed Central PMCID: PMC4004172. Kristensson, K., and Olsson, Y. (1971). Retrograde... PubMed PMID: 25374356. PubMed Central PMCID: PMC4269290. Masamizu, Y., Okada, T., Kawasaki, K., Ishibashi...

  12. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ... PubMed PMID: 26611576. PubMed Central PMCID: PMC4971574. Hooper, Andrew, and Jamie Maguire. "Characterization... PubMed PMID: 26934955. PubMed Central PMCID: PMC4987841. Lodish, Harvey et al. Molecular Cell Biology... PubMed PMID: 24700321. PubMed Central PMCID: PMC4100247. Taylor, Norman E., et al. "Optogenetic activation... PubMed PMID: 22196724. PubMed Central PMCID: PMC4166436. Resources on the Addgene blog Learn more about...

  13. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ... PubMed PMID: 25713381. PubMed Central PMCID: PMC4364232. Find the plasmids from this publication at ... PubMed PMID: 27088723. PubMed Central PMCID: PMC4864854. Find the plasmids from this publication at ... PubMed PMID: 26344044. PubMed Central PMCID: PMC4666719. Find plasmids from this publication at Addgene...

  14. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ... PubMed PMID: 26344044. PubMed Central PMCID: PMC4666719. 4. Mali, Prashant, et al. "RNA-guided human ... PubMed PMID: 26436575. PubMed Central PMCID: PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin... PubMed PMID: 25263330. PubMed Central PMCID: PMC4265475. Additional Resources on the Addgene Blog Listen...

  15. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ... Pubmed PMID: 24733905. Pubmed Central PMCID: PMC4000813. 2. Taylor, Justin Q., et al. “Avoiding a lost... Pubmed PMID: 27175853. Pubmed Central PMCID: PMC4866822. 3. McDowell, Gary S., et al. "Shaping the Future... Pubmed PMID: 25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations...

  16. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ... PubMed PMID: 26837755. PubMed Central PMCID: PMC4905529. Schmid-Burgk, Jonathan L., et al. “CRISPaint... PubMed PMID: 27465542. PubMed Central PMCID: PMC4974478. Natsume, Toyoaki, et al. “Rapid Protein Depletion... PubMed PMID: 27172193. PubMed Central PMCID: PMC4889673. Additional Resources on the Addgene Blog Learn...

  17. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...44. PubMed PMID: 7597057. PubMed Central PMCID: PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon...95. PubMed PMID: 7624356. PubMed Central PMCID: PMC41457. 8. Schnütgen, Frank et al. 2003. “A Directional... PubMed PMID: 26853302. PubMed Central PMCID: PMC4760889. Additional Resources on the Addgene Blog Learn...

  18. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... PubMed PMID: 25337688. PubMed Central PMCID: PMC4244833. Kellenberger, Colleen A., et al. "RNA-based ... PubMed PMID: 27446908. PubMed Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein... PubMed PMID: 25026079. PubMed Central PMCID: PMC4143336. Additional resources on the Addgene blog Learn...

  19. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... PubMed PMID: 25980573. PubMed Central PMCID: PMC4461904. 9. Huang, Q., Mao, Z., Li, S., Hu, J., & Zhu... PubMed PMID: 26224555. PubMed Central PMCID: PMC4884002. 10. Boer, E. de, Rodriguez, P., Bonte, E., Krijgsveld... PubMed PMID: 25993512. PubMed Central PMCID: PMC4439073. 12. Ludwig, L. B., Hughes, B. J., & Schwartz...

  20. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ... PubMed PMID: 26492139. PubMed Central PMCID: PMC4862005. Jiang, Wenyan, et al. "RNA-guided editing of...2015): e83-e83. PubMed PMID: 25800749. PMCID: PMC4513841. Latynski US, Valentovich LN. "DNA tuner: a computer...PubMed PMID: 26002895. PubMed Central  PMCID: PMC4495200. Reisch, Chris R., and Kristala LJ Prather. "...
Showing: 1 - 20 of 125 results