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  1. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ... PubMed PMID: 18818365. PubMed Central PMCID: PMC3987909. 6. Ban, H., et al., Efficient generation of ... PubMed PMID: 21821793. PubMed Central PMCID: PMC3161531. 7. Yu, J., et al., Human induced pluripotent... PubMed PMID: 20888316. PubMed Central PMCID: PMC3656821. 10. Aasen, T., et al., Efficient and rapid generation... PubMed PMID: 21151107. PubMed Central PMCID: PMC3033971. 18. Huang, S.X., et al., Efficient generation... PubMed PMID: 21434814. PubMed Central PMCID: PMC3161101. 20. Chang, C.J., et al., Production of embryonic... PubMed PMID: 22022444. PubMed Central PMCID: PMC3192723. 21. Grigoriadis, A.E., et al., Directed differentiation... PubMed PMID: 22645348. PubMed Central PMCID: PMC3390875. 25. Patsch, C., et al., Generation of vascular...

  2. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ... Pubmed PMID: 21716290. Pubmed Central PMCID: PMC3775282. 2. McDevitt, Ross A., et al. "Serotonergic versus... PubMed PMID: 23473324. PubMed Central PMCID: PMC3595120. 4.Mattis, Joanna, et al. "Principles for applying... PubMed PMID: 22153370. PubMed Central PMCID: PMC3282061. 7.Diester, Ilka, et al. "An optogenetic toolbox... PubMed PMID: 21278729. PubMed Central PMCID: PMC3150193. 8.Han, Xue, et al. "A high-light sensitivity... PubMed PMID: 21811444. PubMed Central PMCID: PMC3082132. 9.Del Bene, Filippo, and Claire Wyart. "Optogenetics... PubMed PMID: 21240279. PubMed Central PMCID: PMC3032981. Resources at Addgene A Primer on Optogenetics...

  3. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    .../j.tics.2011.10.003.  PMID: 22055387;  PMCID: PMC3225502. 4. Mattis J, et al. Principles for applying ...doi: 10.1038/nn.3078.  PMID: 22446880; PMCID: PMC3337962. 8. Zhao S, et al. Cell type-specific channelrhodopsin...methods. 2011;8(9):745-52. PMID: 21985008; PMCID: PMC3191888. 9. Zeng H, Madisen L. Mouse transgenic approaches...0-444-59426-6.00010-0. PMID: 22341327; PMCID: PMC3433654. 10. van Zessen R, et al. Activation of VTA GABA.../j.neuron.2012.02.016. PMID: 22445345; PMCID: PMC3314244. Resources at Addgene Find optogenetics plasmids...

  4. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... Pubmed PMID: 23971632. Pubmed Central PMCID: PMC3806368. 5. Costantini, Lindsey M., et al. "A palette... Pubmed PMID: 21255213. Pubmed Central PMCID: PMC3079558. 8. Suzuki, Takahisa, et al. "Development of ... Pubmed PMID: 22649538. Pubmed Central PMCID: PMC3359384. 9. Costantini, Lindsey M., et al. "Cysteineless... Pubmed PMID: 23257162. Pubmed Central PMCID: PMC3552020. 10. Paladino, Simona, et al. "Protein oligomerization...

  5. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ... PubMed PMID: 23817127. PubMed Central PMCID: PMC3713494. Ghigo, Clément, et al. "Multicolor fate mapping...PubMed PMID: 23940255.  PubMed Central PMCID: PMC3754858.  Hadjieconomou, Dafni, et al. "Flybow: genetic... PubMed PMID: 21205846. PubMed Central PMCID: PMC3082469. Tarlow, Branden D., Milton J. Finegold, and ... PubMed PMID: 22190486. PubMed Central PMCID: PMC3258636.  Additional resources on the Addgene blog Read...

  6. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ... PubMed PMID: 23086203. PubMed Central PMCID: PMC3699407. Milo, Ron. "What is the total number of protein... PubMed PMID: 24114984. PubMed Central PMCID: PMC3910158. Ramanathan, Muthukumar, et al. "RNA–protein ... PubMed PMID: 22412018. PubMed Central PMCID: PMC3308701. Rhee, Hyun-Woo, et al. "Proteomic mapping of... PubMed PMID: 23371551. PubMed Central PMCID: PMC3916822. Schopp, Isabel Myriam, et al. "Split-BioID a...

  7. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...-13. Epub 2013 Aug 30. PMID: 23995928; PMCID: PMC3811519.Seo SO, Schmidt-Dannert C. Development of a synthetic... PubMed PMID: 22205518. PubMed Central PMCID: PMC3574576. Lai, Sen-Li & Tzumin Lee. “Genetic mosaic with... PubMed PMID: 22406855. PubMed Central PMCID: PMC3846601. Find plasmids from this publication at Addgene.... PubMed PMID: 6337371. PubMed Central PMCID: PMC393301. Da Silva, Nancy A. & Sneha Srikrishnan. “Introduction...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... PubMed PMID: 24086725. PubMed Central PMCID: PMC3785459. Beier, K.T., Saunders, A., Oldenburg, I.A., ... PubMed PMID: 21825165. PubMed Central PMCID: PMC3174680. Buttry, J.L., and Goshgarian, H.G. (2015). WGA-Alexa... PubMed PMID: 22196330. PubMed Central PMCID: PMC3275419. Maday, S., Twelvetrees, A.E., Moughamian, A.... PubMed PMID: 22418061. PubMed Central PMCID: PMC3381869. Schmued L.C., and Fallon J.H. (1986). Fluoro-Gold...

  9. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... PubMed PMID: 21628464; PubMed Central PMCID: PMC3138296. 2. Sparta DR, et al. Construction of implantable... PubMed PMID: 23042500; PubMed Central PMCID: PMC3466483. 4. Kim TI, et al. Injectable, cellular-scale... PubMed PMID: 23580530; PubMed Central PMCID: PMC3769938. 5. Iwai Y, et al. A simple head-mountable LED...

  10. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ... PubMed PMID: 24201379. PubMed Central PMCID: PMC3821016. Fujita, Toshitsugu, et al. "Identification of... PubMed PMID: 23849981. PubMed Central PMCID: PMC3770145. Hoshino, Akemi, and Hodaka Fujii. "Insertional... PubMed PMID: 23452860. PubMed Central PMCID: PMC3664290.  Zhang, Yiqun, et al. "CRISPR Cas9-guided chromatin...

  11. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ....PubMed PMID: 21838686. PubMed Central PMCID: PMC3205285. 7. Ouellet, Jonathan. “RNA Fluorescence with... PubMed PMID: 22368488. PubMed Central PMCID: PMC3279232. 10. Song, Shiping, et al. “Aptamer-based biosensors... PubMed PMID: 21838688. PubMed Central PMCID: PMC3205236. 13. Xiao, Saijin, et al. “Aptamer-based assay...

  12. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ... PubMed PMID: 22428975. PubMed Central PMCID: PMC3640491. 2. Grimm, D., et al. "Titration of AAV-2 particles... PubMed PMID: 22035192. PubMed Central PMCID: PMC3216358. 4. Lock, Martin, et al. "Characterization of... PubMed PMID: 23828206. PubMed Central PMCID: PMC3706409. 7. Zeltner, Nadja, et al. "Near-perfect infectivity...

  13. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ... PubMed PMID: 24253446. PubMed Central PMCID: PMC3875854. 2. Mashiko, Daisuke, et al. "Generation of mutant... PubMed PMID: 24284873. PubMed Central PMCID: PMC3842082. 3. Mashiko, Daisuke, et al. "Feasibility for... PubMed PMID: 23643243. PubMed Central PMCID: PMC3969854. Additional Resources on the Addgene Blog Get...

  14. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    .... PubMed PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating... PubMed PMID: 14704360. PubMed Central PMCID: PMC373311. 3. Kim, Jung-Sik, et al. "Epitope tagging of ... PubMed PMID: 22108859. PubMed Central PMCID: PMC3277219. 6. Ohmori, Tsukasa, et al. "CRISPR/Cas9-mediated...

  15. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    .... PubMed PMID: 23924161 PubMed Central PMCID: PMC3783214.  10. Hilaire, Mary Rose, et al. "Blue fluorescent... PubMed PMID: 24360272. PubMed Central PMCID: PMC3918502.  14. Chernov, Konstantin G., et al. "Near-infrared... PubMed PMID: 21619590. PubMed Central PMCID: PMC3129320.  18. Mastop, Marieke, et al. "A FRET-based biosensor...

  16. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... PubMed PMID: 24086725. PubMed Central PMCID: PMC3785459. 4. Castle, Michael J., et al. "Adeno-associated... PubMed PMID: 21102559. PubMed Central PMCID: PMC3098627. 6. Salegio, Ernesto A., et al. "Axonal transport... PubMed PMID: 22418061. PubMed Central PMCID: PMC3381869. 7. McCarty, D. M., Paul E. Monahan, and Richard...

  17. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... PubMed PMID: 23792628. PubMed Central PMCID: PMC3773023. 2. Vouillot, Léna, Aurore Thélie, and Nicolas... PubMed PMID: 23934178. PubMed Central PMCID: PMC3782611. 8. Tsai, Shengdar Q., et al. "GUIDE-seq enables... PubMed PMID: 23503052. PubMed Central PMCID: PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "...

  18. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    .... PubMed PMID: 7041091. PubMed Central PMCID: PMC320540. 10. Goffin, Colette, Veronique Bailly, and Walter.... PubMed PMID: 3684572. PubMed Central PMCID: PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity... PubMed PMID: 23720790. PubMed Central PMCID: PMC3719660. 20. Watanabe, Sho, et al. "Microbead-based ligase...

  19. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ... PubMed PMID: 24043817. PubMed Central PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics... PubMed PMID: 14219055. PubMed Central PMCID: PMC314833. Datta, Naomi. “Transmissible Drug Resistance ...

  20. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ... PubMed PMID: 24086725. PubMed Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams... PubMed PMID: 21364278. PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin...
Showing: 1 - 20 of 98 results