...larger epic of fantasy novels. Set in a world where 1000 years ago the hero lost and the Dark Lord rules ...Napoleonic Wars and against something even more sinister. 1000 pages long. Incredibly imaginative, funny, bittersweet...emotions, and even what therapeutic properties it has. As with other works of Dr. Sacks, Musicophilia includes... pretty biased as the founder, but our book club has become one of my favorite parts of Addgene’s culture...

...indicates that a plasmid has been requested 20+ times   A red flame means a plasmid has been requested 50+ ... but a select few are particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested... Castro of University of Michigan says, “Addgene has transformed the way in which we share plasmids and...making it easier, cost effective and faster; this has helped accelerate scientific discoveries.” Occasionally...+ times   A blue flame means a plasmid has been requested 100+ times Since we so sincerely appreciate ...have at least one plasmid deposited at Addgene that has been distributed more than 100 times. We hope these...sharing because, as UNC’s Bryan Roth says, Addgene has “literally enabled sharing with 100's of investigators...

...barcodes. Rather than continuing to process 100s to 1000s of samples individually, plexWell™ reduces every...with our plasmid assembly pipeline. To date, this has resulted in high quality NGS-based assemblies for...

...five years, inflation has occurred. Here at Addgene, the consumables cost per stab has increased 50% since... very cool 8,768,560 hours – or almost exactly 1000 calendar years!   *Dammit, man, I’m a scientist, ...make a plasmid. Doench’s lab in the Broad Institute has created hundreds of thousands of plasmids, using ...since 2019, while overall inflation of USD has gone up 19.8% in the same time period. For ease of calculations...per plasmid, Addgene, since its founding in 2004, has saved the scientific community a collective and very...

...pieces of roughly equal size, typically between 200-1000 bp. This is to ensure that your downstream analysis...an antibody for the first time, look for one that has been validated in multiple assay types. Remember ...

...between a cell that has had one viral particle integrated into its genome and a cell that has had multiple integration...particles. Studies have found that values can be 10-1000 fold higher than for functional assays depending...The day has arrived; you’ve painstakingly cared for your packaging cell line, prepped your DNA, transfected... be significantly higher than the same prep that has been stored in the freezer. If you plan to freeze..., you may want to consider using an aliquot that has been subjected to a freeze/thaw cycle for titering...CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage Tracing Additional Resources...

...proximity ligation." Nature methods 3.12 (2006): 995-1000. PubMed PMID: 17072308.  4. Dieck, Tom, et al. "...Singer’s lab at Albert Einstein College of Medicine has recently published techniques to follow translation...Rojas! Eugenia Rojas, PhD is a neuroscientist who has always been deeply interested in neurobiology.  She...

...coefficient and quantum yield of the protein, divided by 1000. In many cases the brightness is compared to that...Fluorescence is the emission of light by a substance that has absorbed light. The emitted light is at a longer ...protein Excitation & Emission (ex/em): Each FP has its unique ex/em peak. Therefore, choose FPs that...detect the emission. For example, if your microscope has only two lasers, at 488nm and 561nm, you will not...Some proteins are very dim (e.g. TagRFP657, which has a brightness of 0.1) and this should be taken into...

...used to calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies/uL V = Volume...we should first note that quantitative PCR (qPCR) has been a powerful tool for quantifying AAV for some... 2 minutes and the denaturation time to 1 minute has been shown to increase droplet separation (Witte ...

... (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target...transcript, you will also need to make sure your plasmid has a promoter suited to working in your host organism... but requires SP6 RNA polymerase. SP6 polymerase has a high processivity. When used for in vitro transcription...

...Beroukhim and Boehm labs pulled data from more than 1000 CRISPR screens in DepMap to see if they could identify...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...

...includes SpCas9, and shortern orthologs approximately 1000 amino acids in size. From the pool of shorter orthologs... 16, 2020 by Beth Kenkel.  CRISPR genome editing has quickly become a popular system for in vitro and ...packaging capacity of AAV (~4.7 kb). While this approach has been proven feasible, it leaves little room for additional...the number of targetable loci, and truncated Cas9 has much lower efficiency than its wild-type counterpart...amino acids, can also be packaged into AAV. NmeCas9 has the added advantage that it can be turned off by ...daunting, it’s actually a challenge that the field has overcome with split AAVs. In general split AAVs break...

...of template should have homology arms between 500-1000 bps. HDR efficiency with circular plasmid templates...applicable in DSB-induced genome engineering, it has biological importance for the repair of breaks that...directed repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made...

...design 5 guide RNAs (gRNAs) targeting each of the 1000 genes you’d like to investigate in your next CRISPR... population level, but each individual cell only has a maximum of one gene knocked out. In this genetically...containing up to around 200,000 different gRNAs. It has been suggested that researchers should use ~400X ...than gRNAs in a screen, to ensure that each gRNA has a good chance to contribute to the screen. In the... If you are lucky and find that a druggable gene has a central role in your system, you could majorly ...guest blogger Fredrik Wermeling! Fredrik Wermeling has a research group at the Centrum for Molecular Medicine...Medicine, Solna, Karolinska Institutet, in Sweden. He has a particular interest in using molecular tools to...

...Low 572/ 610 FP595 Tetramer Green Dark/ Red 70 - 1000 Medium 590/ 600   Choosing a photoactivatable...high contrast ratio usually indicates that a PA-FP has a low level of spontaneous fluorescence in the absence...

... E. coli.” ACS Synthetic Biology 4(9) (2015):987-1000. PubMed PMID: 25822415. Find plasmids from this...multiple gRNAs on one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll use mammalian...

...protein–protein interactions. The Roux lab has deposited HA tagged BioID2 for N-terminal fusions and Myc...produces point mutations at a rate of up to 1 per 500-1000 bp. In comparison, the DNA replication error rate... activation of these receptors. Bryan Roth's lab has a newly available PRESTO-TANGO kit which allows researchers...the NCI RAS Initiative, led by Dominic Esposito, has recently released a collection of 360 Ras pathway...24395366 Oxford Drosophila CRISPR library Addgene has had the privilege of distributing several human and...this 3-day goal is is now attainable. The Jacks lab has recently introduced a new assembly cloning platform...Addgene to help expand the collection! The Jacks lab has made several GMAP-compatible plasmids available to...

...plasmid #), and other reagents. F1000Research, among many other journals, has joined a pilot project from ... up their own minds on whether the data analysis has been of good quality and whether they agree with ...Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes taking place in the world...results more valuable. Some journals, including F1000Research, also ask for the raw data behind the figures...several rounds of revision before acceptance. F1000Research uses a novel form of peer review that occurs... Maaike Pols is a Developmental Editor for F1000Research, responsible for assessing the scientific content...

... a raise in salary, particularly given that this has precipitated increases to the NIH recommended pay...both of these changes share a common thread that has the potential to profoundly shift the academic science...rather than as trainees in a special category, as has been the ‘labor-of-love’ philosophy of academic science...sector throughout their careers (McDowell et al, F1000Research, 2014; Dolan, F100Research, 2016). These events...Research: a perspective from junior scientists."F1000Research 3 (2014). Pubmed PMID: 25653845. Pubmed Central...from the Future of Research Chicago Symposium F1000Research, 2016. Kyle T. Dolan, Joseph F. Pierre and Erin...

...Cas9, but the ever-expanding repertoire of CRISPR has brought many other Cas effectors to light. One lesser-known...effector, and type IV with an unknown effector. Type I has subtypes A through G, with different compositions... Thanks to this complex, developing Cas3 tools has been challenging. It can be difficult to ensure comparable... job — Cas see DNA, Cas chop DNA. Cas3, however, has two jobs, one as a nuclease and one as a helicase...easier to work with than Cas3. Future targets Cas3 has the potential to be incredibly useful in therapeutics...and Genome Editing, 3–4, 100013. https://doi.org/10.1016/j.ggedit.2022.100013 O’Brien, R. E., Bravo, J...