...GBPs/GFP. The new system, CRE-DOG (Cre Dependent On GFP), is activated by GFP and derivatives GFP and ...to bind GFP. The idea of using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate...presence of GFP. These systems, known as T-DDOG and Cre-DOG, respectively, repurpose popular GFP reporter...lines expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted...co-opted to control gene expression, he could then selectively manipulate only GFP-labeled cells. Once Tang ...tested pairs of their GFP-binding proteins (GBPs) to find those that could co-occupy GFP. Once they’d found...culture system. In cells cotransfected with GFP and the GBPs, GFP linked the two GBPs together to create a...

...those formed from control (GFP gRNA-targeted) BRAF sites (lanes 3, 4) were used as controls. Duplexes were...from BRAF-edited and GFP-edited amplification products (BRAF-GFP duplex) and from GFP-edited amplification...amplification products (GFP-GFP duplex) as a control. 4. After optimizing the reaction and confirming the presence...express CAs9 and are included as a control (WT). Vinculin is a loading control. 3. In terms of making the... for gene editing. A virus encoding GFP plus a gRNA targeting GFP (pXPR_011, 59702-LV) was used to assay... by the virally-delivered GFP gRNA, will edit out the virally-delivered GFP gene. So, cells with no Cas9...I thought I could see GFP expression with a fluorescence microscope, but the GFP expression was so low...

...many of our control plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for .... Find the Dolcetto lentiviral prep here Control AAV Control AAV allow researchers to not only interpret...mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control offered by the...respectively of a GFP dependent Cre. The two segments come together in the presence of GFP to form a functional... activation to cells expressing both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469), is also available...-2 proteins ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral...for their own experiments. To this end, we offer control AAV in smaller aliquots of 20ul for serotype testing...

...scientists to begin to study GFP and deduce its biochemical properties. Now GFP and its fluorescent derivatives...fusions. Transcription Reporter: Placing GFP under the control of a promoter of interest can be used to...selection. Plasmids of this type may have the GFP under the control of an additional promoter from that of the...expressing GFP from cells that are not. Developmental/transgenic uses: Because of its stability, GFP can be...Fluorescent Protein Mutations Relative to Wildtype GFP EGFP F64L; S65T EYFP S65G; V68L; S72A; T203Y mYFP...halves of EGFP undergo folding, maturation, and fluorescence. Biosensors: A wide array of GFP-based fluorescent...from proteins such as Green Fluorescent Protein (GFP) has likely existed in creatures such as jellyfish...

...live cells are GFP+. In this plot, with adjusted voltage, cells that did not receive the GFP protein are ...intensity. If your samples and controls were processed the same way, the GFP- population should overlap with... with the negative control population (Figure 1). Based on where the GFP- population ends, you can draw...-case result. For example, you have introduced a GFP protein into your cells (visualized via the FITC ... to be clustered at signals below 103, while the GFP+ population cluster would be shifted to the right...selects anything to the right of it, marking it as GFP+. Quantification for such graphs could simply be ... Example of yes-no flow plot of the (negative) control (left) or with the sample (right). Negative and...

...pAAV.hSyn-FLEX.iGABASnFR.F102G pAAV-Syn-Archon1-KGC-GFP-ER2 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] pAAV-FLEX-tdTomato pAAV-GFAP104...pAAV-mDlx-NLS-mRuby2 CAG-NLS-GFP pAAV-hSyn-mCherry Read on to learn more!  Calcium sensor AAV The GFP-based calcium...pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent version (pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]) of this membrane-embedding...37825  AAV8*, AAV9*  pAAV-CAG-GFP 50465  AAV8*, AAV9*  pAAV-hSyn-EGFP *Also coming in our new 20 ...cre-dependent version at Addgene! Fluorescent protein and control AAV These fluorescent protein expression vectors...interneurons. These vectors can also be used as the control vectors for experiments encoding a fluorescent-...pAAV-GFAP104-mCherry, pAAV-mDlx-NLS-mRuby2, CAG-NLS-GFP,and pAAV-hSyn-mCherry at Addgene. Viral vectors coming...

...popular control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs ...intersectional studies And more! Control AAV We’re always excited to add new control AAVs to our inventory since...about. Controls Plasmid Serotype Name 37825  AAVrg (20 µL size serotype testing) pAAV-CAG-GFP 50465...Serotype Name 105530  AAV1 pAAV.CMV.PI.EGFP.WPRE.bGH 37825  AAV1 pAAV-CAG-GFP 59462  AAV2 pAAV-CAG-tdTomato... testing) pAAV-CAG-GFP 50465 AAV8, 9 (20 µL size serotype testing) AAV-hSyn-EGFP Biosensor AAV The...our entire AAV inventory. Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for calcium ...scientists, we now offer trial size 20 µL samples of control AAV that scientists can use for serotype testing...

...an internal control. Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence...Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy biosensor contains mRFP and GFP fused to the...flux. mRFP-GFP-LC3 functions as an autophagy bionsensor via the same mechanism as Rosella: GFP is quenched...decrease in red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe ... that releases an internal control Another autophagy biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar...molecule of GFP-tagged LC3 for every one molecule of RFP-tagged LC3∆G. Like the unmodified LC3, GFP-LC3 is ...variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super ecliptic pHluorin (SEP). In the absence...

...light-based control of signaling pathways. This is particularly exciting because using light to control signaling...  Option #2 may sound like fiction, but light-controlled protein purification is now a reality thanks ..., and their use for protein purification and controlling cell signaling. Key components of OptoNBs OptoNBs...Lab monitored how AsLOV2 insertion into mCherry or GFP nanobodies affected binding of their target proteins...co-localization with a membrane-localized mCherry or GFP protein. With this system, if the OptoNB can bind...insertion into loops 1 and 6 resulted in light controlled binding, but with opposite effects: insertion...more OptoNBs, one against mCherry and one against GFP. Insertion of AsLOV2 into loop 1 always generated...

...Chang-Deng Hu Green sfGFP Split super-folder GFP, our most-requested GFP(1-10) and GFP(11) Versatile protein...Commun. 2016 Bo Huang spGFP Split superpositive GFP Split-superpositive GFP reassembly is a fast, efficient...live cells.  Controls  When designing experiments, it’s important to include proper controls to assess the...base FP (for example, EBFP2(1-10) can be used with GFP(11)). Some of the articles linked below only provide...Blue EBFP2 EBFP2(1-10) and Capri(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with... Kamiyama Cerulean Cerulean(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with... p53 tetramerization in response to DNA damage controls the p53 response. Gaglia G, Lahav G. Mol Syst ...

...replicate controls.  Transfection controls: empty vector and internal control A transfection control measures...Negative controls: untreated cells, empty vector control, and non-targeting control Negative control conditions...lacking the proper controls are invariably inconclusive and unreliable. Proper controls provide the constant...about the various controls that can be used for plasmid-based experiments. What are control plasmids? Generally...experiment, delineate its requisite control plasmids, and discuss why these control plasmids are critical for the...use of proper control plasmids would address and answer these questions. Types of control plasmids Part...the target cells. One transfection control is an empty vector control; specifically, the plasmid without...

...individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9 technology... in human cell lines. Furthermore, the quality control necessary to ensure the edited cells are behaving...to endogenously tag a wide variety of genes with GFP. These plasmids are available through Addgene, and...is the addition of a useful coding sequence (i.e. GFP) into a gene while leaving other features of the ...we show an example gene targeted for tagging with GFP at the C terminus, resulting in one edited allele...allele, or gene copy. The coding sequence for GFP is transcribed and translated along with the gene, producing...shown in the example above, we inserted a linker and GFP tag at the end of the last exon of the gene to ensure...

... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma Our 293T...virus preps are titered and subjected to quality control by Addgene before being distributed to customers...production protocols, titering methods, and quality control are described below. AAV Production AAV distributed...probes targeting the ITR elements and an internal control of known titer (protocol modified from Lock et ... April 2016) also performed by ddPCR.  Quality Control Addgene ensures high quality viral vectors by optimizing...production protocols and performing rigorous quality control (QC). The specific QC experiments performed varies...

...TOPO E17TAG GFP and pGEX-6P-1. The pCRT7 NT TOPO vector contains a mutant pSer-containing GFP that can be...be used as a positive control during optimization experiments. This version of GFP will contain a phosphoserine...-phosphorylated or ‘wild type’ control protein. The ‘wild type’ control can be made one of two ways. First...instead of a stop codon, which will allow full length GFP to be translated), and second, through a gel shift...protein. Up to 80% p-Ser incorporation into E17TAG GFP has been achieved with the Rinehart system (6); however...expression plasmid. This is a particularly important control when creating a phosphoserine-incorporation mutant...mutating a threonine residue to a phosphoserine) to control for effects of the amino acid substitution. Alternatively...

...pAAV-CAG-GFP AAV9-X1.1 Controls Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls Bryan Roth...CN3905) AAV PHP.eB Controls Jonathan Ting New viral prep pAAV-CaMKIIa-EGFP AAV1 Controls Bryan Roth New ...AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB...minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) AAV PHP.eB Controls Jonathan Ting New viral prep AiP13038 - pAAV-AiE0743m...minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038) AAV PHP.eB Controls Jonathan Ting New viral prep AiP12408 - pAAV-AiE0600m-minBG-SYFP2...pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) AAV PHP.eB Controls Jonathan Ting New viral prep AiP13863 - pAAV-AiE0888m_C4...minBG-SYFP2-WPRE3-BGHpA (Alias: CN3863) AAV PHP.eB Controls Jonathan Ting New viral prep AiP13905 - pAAV-AiE0441h...

...sugars (including lactose), and a lac operon controlled GFP. When the enzyme breaks down the HMO of interest...lactose released activates expression of GFP from the lac operon. GFP expression can then be used to determine...Xin hypothesized that she could make enzymes controllable by tethering them to Dronpa, a fluorescent protein...their ability to be precisely spatio-temporally controlled as a key advantage over other inducible enzyme...Photosactivatable Fluorescent Proteins Use Light to Control CRISPR Genome Editing Choose the Brightest Fluorescent...second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter known to bind...

...organisms to control their movements and behavior. These functions and others are controlled by collections... structures GFP is limited in its ability to label subcellular structures in the CNS. GFP and other FPs...connectomic tracers Fluorescent proteins such as GFP have been used for a long time as probes to study...fluorescent signals or using anti-FP antibodies. GFP emits light in only a single channel. In multi-color...these FPs is that they are less photostable than GFP. In addition, antibody-based amplification of their..., prone to aggregation and cross-react with anti-GFP antibodies, making them difficult to use in a multicolor...with high fidelity and at lower concentrations than GFP. smFPs enable better labeling of these fine structures...

... comparison, capsids were packed with a GFP transgene and GFP intensity and transduction efficiency of...AAV.PHP.eB and AAV.PHP.S had 1.5- to 2-fold higher GFP intensity compared to the strains they were derived...also transduced a higher number of neurons overall (GFP+ cells that also stained for a neuronal marker). ...efficiency of AAV-PHP.B versus AAV-PHP.eB in the CNS. A GFP transgene was packaged into either an AAV-PHP.B or...mouse and the level of transduction, measured via GFP expression, was determined 3 weeks later. Table was...efficiency of AAV.9 versus AAV.PHP.S in the PNS. A GFP transgene was packaged into either an AAV.9 or an...

...researchers explore the complexity of translational control in cellular physiology. Ribosome profiling Ribosome...switching, TRICK requires the following components: a GFP protein that binds to the coding region of a reporter...reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) an RFP protein that binds to the 3’ UTR of the reporter...Plasmid 64542 or 64543) Before translation, both GFP and RFP are bound to the reporter, causing it to ...appear yellow. The act of translation pushes NLS-PCP-GFP off of the mRNA, leaving only a red FP bound to the... contains a nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind cognate untranslated...verified that translation is necessary for removal of GFP from the TRICK reporter. Delayed translation is known...

...released Anti-GFP [N86/8R] as another option for targeting GFP. But why might you need a second GFP antibody...PMCID: PMC9015817.   Meet anti-GFP [N86/8R]! by: Ashley Waldron Anti-GFP [N86/38.1R] was among the first...western blot for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP [N86/8R] page...system Heat-inducible lentiviral Car constructs Anti-GFP [N86/8R] ready-to-use antibody Hyperfolder YFP ...heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity of CAR-T cells within tumours via...?  Similarities:  Generated against full length GFP and converted to recombinant form by the NeuroMab...variable domains that recognize different epitopes on GFP Monoclonal antibodies can yield relatively low signal...