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  1. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ... Conferring Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin bsd  HeLa, ...plasmid contains a cassette that will make cells resistant to a toxin), whereas negative selection would ...reagent). Cells should die within 3-5 days and resistant colonies appear in about 10-14 days depending ...confusing since the neo/kan gene confers G418 resistance; however, like gentamicin, neomycin is typically...Backbone Read More: Plasmids 101: Antibiotic Resistance Genes Plasmids 101: Origin of Replication All... antibiotic resistance...
  2. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...determined by transposition assays of antibiotic-resistance genes conducted in HeLa cells). In their Nature...16 variants with different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent...
  3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ..., or other. Bacterial Resistance Enter bacterial antibiotic resistance encoded in the plasmid that...glycerol stock. Choose from: ampicillin, apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin...
Showing: 1 - 4 of 4 results