...de Eyto E, Hampton SE, Keller PS, Sharma S, Lewis ASL, Weyhenmeyer GA, O’Reilly CM, Lofton ME, Catalán ...

...OptoNBs have two key components: an AsLOV2 domain and a nanobody.  AsLOV2 is a light-oxygen-voltage sensing...important to insert AsLOV2 at a spot in the nanobody that preserves the activity of both AsLOV2 and the nanobody... the oat plant (Avena sativa). In darkness, the AsLOV2 domain folds back on itself in a closed conformation...interact? When fused together to form an OptoNB, the AsLOV2 domain acts like an on/off switch for the nanobody...Generating OptoNBs The opening and closing of the AsLOV2 domain allosterically alters the shape and binding...proof-of-principle the Toettcher Lab monitored how AsLOV2 insertion into mCherry or GFP nanobodies affected...loops of nanobodies seemed like a good place to put AsLOV2 because here it was less likely to destroy the ...

...had shown magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random mutagenesis...she deprioritize the project, Maria’s screens of AsLOV2 variants showed some significant gains! After a...mutagenesis, they eventually found a version of AsLOV2 with five mutations (C450P, L496V, Q513K, G528K...Fluorescence intensity and ΔF/F of MagLOV, a variant of AsLOV2 with mutations C450P, L496V, Q513K, G528K, D540M...optogenetic tools. And, since MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic...

...deposited for the photoswitchable AsLOV2. Find the cPYP and AsLOV2 protein binder plasmids here! Reis...binder tools for photoswitchable proteins cPYP and AsLOV2 Article contributed by Angela Abitua  Listen to...

...Sativa phototropin 1 (AsLOV2). In the dark, this peptide is caged by the asLOV2 domain and has reduced...target, SspB. In the light, the Jα helix of the asLOV2 domain unfolds allowing it to bind to SspB. This...

... for example, if someone is awake, agitated, or asleep. Though DA firing patterns (or the frequency of...

...complex biological systems. He can be contacted at aslw@hku.hk.   References 1. Cheng, Allen A., Huiming...

...R., Xiang, L., Fang, X., Jin, Z., Goldblum, D., Hasler, P.W., Scholl, H.P., Krol, J., & Roska, B. (2019...

...communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube channel @Ehmee, and...

...Björklund M, Ahr D, Ahmed AW, Spinelli L, Brun C, Basler K, Merabet S (2018) Generation of a versatile BiFC...

...other viruses that cause disease in humans such as measles, mumps, and rabies. Research on Ebola virus has...

...reduce solubililty HA YPYDVPDYA or YAYDVPDYA or YDVPDYASL  1.1 Affinity Frequently used for western blots...

...March for Science Website Locke, John, and Peter Laslett. Locke: Two treatises of government student edition...

...Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional...

...Paramyxoviridae family of viruses, which also includes measles and mumps. Sendai transduces a wide range of cell...