...controls Ubiquitously expressed proteins like β-actin or GAPDH are popular choices for loading controls...Western bot of liver lysate (10–40 µg) probed with β-actin (1:2000). Representative of three independent blots... Graphs showing the relative intensity of the β-actin (B) or total protein (C) on the membrane versus ...protein staining is a superior loading control to β-actin for Western blots,” pages 186–188, copyright 2013...difficult to see an increasing concentration of β-actin in panel A. Indeed, the quantification in panel ...-axes between panels B and C, indicating that β-actin is less sensitive as well as less linear than the...protein staining is a superior loading control to β-actin for Western blots. Analytical Biochemistry, 440(...

...ubiquitous. human beta actin General expression mRNA Mammalian promoter from beta actin gene Constitutive  ...Constitutive  Contains CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice acceptor...expression mRNA Strong insect promoter from Drosophila Actin 5c gene Constitutive  Commonly used in expression...

...cellular contraction. This network is composed of actin and myosin which interact with each other to generate...necessary for myosin activity and thus interaction with actin in nonmuscle systems in the fly. Spaghetti squash... an idea: sqh mutant fly ovaries had “squiggly” actin fibers that resemble spaghetti. We have two plasmids...7915669. Dominguez, Roberto, and Kenneth C. Holmes. "Actin structure and function." (2011). PubMed PMID: 21314430...

...Micrographs of a fluorescently-labeled actin filament (488-Actin, cyan), with formin (649-mDia1, magenta...   Figure 3: Single-molecule tracking of actin filament assembly-regulating proteins in vitro reveals...mDia1 or 549-CP (upper, middle) and the effects on actin filament length (lower). d) Intensity and filament...Shekhar, S. (2023). Multicomponent regulation of actin barbed end assembly by twinfilin, formin and capping...became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR: The Virginia...

...most-important component of the cytoskeleton – actin? Actin dynamics control cell morphology, movement, ...endothelium, but they could be applied to study other actin-dependent processes in cells and tissues. And, this...

... Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin...

...detects a ubiquitously expressed protein, such as actin. Actin levels should be consistent across all samples...horseradish peroxidase (HRP), that emits light upon reacting with a specific substrate. The emitted light, ...

...proteins with mEmerald first, Baird says. From F-Actin to mitochondria, from the nucleus to cell membrane...

...use a tag? If you are fortunate enough to study actin, then you may not need to read this - but for many...

...4x-FLAG-mCherry-H2B), cytoskeletal (middle, 4xFLAG-mCherry-β-actin), and mitochondrial (mito-mCherry-1xFLAG) localization...

...Adina R., Bin Wu, and Robert H. Singer. "Single β-actin mRNA detection in neurons reveals a mechanism for...

...for ATP or other chemical fuel sources. Like an actin filament or a microtubule, R bodies are a polymer...

...: GFP, RFP,  the histone protein H2A2B, and the actin-binding protein gelsolin, but any nanobody sequence...

...promoter consisting of a CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice acceptor...

...doesn’t mean we should start re-conceptualizing actin filaments or microtubules. This issue is shared ...

...plasmids, or promoters such as the chicken beta-actin promoter of the pHLsec vector. In this scenario,...

...mechanical forces in the cell’s microenvironment. The actin cytoskeleton exerts tensile force, transmitted through...

...polarization independent of GDI-mediated extraction and actin-based trafficking." PLoS biology 13.4 (2015): e1002097...

...different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression, but...

...have been transfected with a plasmid encoding GFP. Actin, a common internal control for Western blots, shows...