...ubiquitous. human beta actin General expression mRNA Mammalian promoter from beta actin gene Constitutive  ...Constitutive  Contains CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice acceptor...expression mRNA Strong insect promoter from Drosophila Actin 5c gene Constitutive  Commonly used in expression...

...cellular contraction. This network is composed of actin and myosin which interact with each other to generate...necessary for myosin activity and thus interaction with actin in nonmuscle systems in the fly. Spaghetti squash... an idea: sqh mutant fly ovaries had “squiggly” actin fibers that resemble spaghetti. We have two plasmids...7915669. Dominguez, Roberto, and Kenneth C. Holmes. "Actin structure and function." (2011). PubMed PMID: 21314430...

...Micrographs of a fluorescently-labeled actin filament (488-Actin, cyan), with formin (649-mDia1, magenta...   Figure 3: Single-molecule tracking of actin filament assembly-regulating proteins in vitro reveals...mDia1 or 549-CP (upper, middle) and the effects on actin filament length (lower). d) Intensity and filament...Shekhar, S. (2023). Multicomponent regulation of actin barbed end assembly by twinfilin, formin and capping...became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR: The Virginia...

...most-important component of the cytoskeleton – actin? Actin dynamics control cell morphology, movement, ...endothelium, but they could be applied to study other actin-dependent processes in cells and tissues. And, this...

... Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin...

...detects a ubiquitously expressed protein, such as actin. Actin levels should be consistent across all samples...horseradish peroxidase (HRP), that emits light upon reacting with a specific substrate. The emitted light, ...

...proteins with mEmerald first, Baird says. From F-Actin to mitochondria, from the nucleus to cell membrane...

...use a tag? If you are fortunate enough to study actin, then you may not need to read this - but for many...

...Adina R., Bin Wu, and Robert H. Singer. "Single β-actin mRNA detection in neurons reveals a mechanism for...

...4x-FLAG-mCherry-H2B), cytoskeletal (middle, 4xFLAG-mCherry-β-actin), and mitochondrial (mito-mCherry-1xFLAG) localization...

...for ATP or other chemical fuel sources. Like an actin filament or a microtubule, R bodies are a polymer...

...: GFP, RFP,  the histone protein H2A2B, and the actin-binding protein gelsolin, but any nanobody sequence...

...promoter consisting of a CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice acceptor...

...plasmids, or promoters such as the chicken beta-actin promoter of the pHLsec vector. In this scenario,...

...doesn’t mean we should start re-conceptualizing actin filaments or microtubules. This issue is shared ...

...polarization independent of GDI-mediated extraction and actin-based trafficking." PLoS biology 13.4 (2015): e1002097...

...mechanical forces in the cell’s microenvironment. The actin cytoskeleton exerts tensile force, transmitted through...

...have been transfected with a plasmid encoding GFP. Actin, a common internal control for Western blots, shows...

...different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression, but...

...expressed “housekeeping” proteins — like GAPDH or actin — are often used, it is far more accurate to use...