... Alexander A. Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little...of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with the Zika virus research...albopictus) and, aided by international travel, the Zika virus is expected to expand into heavily populated...e.g.: blood, urine). Adapting toehold switches for Zika detection We and other members of the lab had been...platform toward a more field-ready format when the Zika virus outbreak was reported, and we felt that, given...manuscript to the journal Cell just six weeks later. The Zika publication timeline I think we can speak for all...platform into clinically relevant diagnostics for the Zika virus was daunting. It took a true team effort and...

...they function. Adapting Toehold Switches to Detect Zika Virus The RNA-based toehold switches described in...are engineered to bind RNA sequences derived from Zika virus and, upon binding, produce reporter proteins...switches can be used to quickly detect the presence of Zika virus. Read this post from Keith Pardee and Alexander...these amazing tools may be used to rapidly diagnose Zika virus infection. Microbiology and virology Minigenomes...

...decreased Zika viral RNA levels by 90%, suggesting that QKI is a host protein critical to Zika replication...proteins interact with viral RNAs. RNA viruses, like Zika, often trick host proteins into binding their untranslated...used RaPID to see what host proteins interact with Zika’s UTR and identified QKI, an RNA-binding protein ...

...multiple patients was published, Zika researcher Christa Osuna collated Zika case studies, making a meta analysis...

...that SHERLOCK could reliably distinguish between Zika and a closely-related virus, Dengue from multiple...Blog Learn about using toehold switches to detect Zika virus   Learn more about targeting RNA with Cas13a...

... for Biology Adapting Toehold Switches to Detect Zika Virus As always, if you have any questions or suggestions...

...Green has also developed toehold switches to detect Zika virus (available at Addgene). The plasmids for detecting...

...figure 1) has been used to differentiate strains of Zika virus, genotype human DNA, identify tumor mutations...

...unpublished set of Zika gene plasmids to Addgene’s repository. The genes, from the Zika virus Asian genotype.... 2016. PubMed PMID: 26837755   SEPTEMBER 2016 Zika virus plasmids Now avaliable through Addgene The...