...#49004) to the spectra of both mCherry and TagRFP.  Here, the TagRFP spectrum is shown in the darker colors...peak of the TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission ...or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent protein such as iFP1.4... emission. For this filter set, we would expect TagRFP to give a brighter signal than mCherry. In general...shorter wavelength red fluorescent proteins like TagRFP or mRuby2 than longer wavelength proteins like ...

...change that placesTagRFP-T and Dronpa in close proximity. By monitoring changes in TagRFP-T fluorescence...FLINC)-based biosensors measure fluctuations of TagRFP-T fluorescence intensity due to interactions with...membrane in living cells using a construct in which TagRFP-T and Dronpa flanked a PKA substrate fused to the...

...better understand T cell initiation in the adaptive immune response. It was known that T cells were able... fast T cells could jump from one antigen presenting cell to another. Indeed, we now know that T cells...like EGFP Yellow-orange fluorescent proteins like TagRFP, tdTomato, DsRed, the mKate series, or tdKatushka2...constructs in the immune lineage. By specifically labeling T cells, B cells, and antigen presenting cells, scientists...answer using two-photon microscopy include: How do T cells and/or Neutrophils travel to the sites of infection...dendritic cells migrate to lymph nodes to activate T cells (Celli et al 2008, Kitano et al. 2016, Cavanagh...example, have been successfully used to study B and T cell dynamics within the germinal center of murine...

...can fold in <10min at 37°C, mCherry takes ~15min, TagRFP ~100min and DsRed ~10hours. Temperature: FPs maturation...their emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...is set as 1. Some proteins are very dim (e.g. TagRFP657, which has a brightness of 0.1) and this should...

...oligomers such as DsRed or high affinity FPs such as TagRFP. Problem 3 can be solved, in most cases, by using...10): p. 969-73. PubMed PMID: 10504696. 4. Ohashi, T., et al., An experimental study of GFP-based FRET,...

... of the toolkit in action, see Cermak T, et al. 2017. Cermak T, et al. Plant Cell. 2017. PubMed PMID:...extra-chromosomal telomeric DNA), which accumulates when T-loops (secondary telomeric structures) are deleted...emission spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors...