...fluorescent signals. Named for the "stellar explosion SUperNova," SunTag can help you turn up the brightness in... al. observed some GFP aggregation, which they reduced by using superfolder GFP (sfGFP) with the small... fusion could be used as well. Pros of SunTag compared to traditional fluorescent proteins Improved ...signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule...

...He et al also compared MG-2I-dL5** head-to-head with KillerRed, a genetically encoded red fluorescent photosensitizer...Fluorescent Protein Guide Page Check out MiniSOG2 and SuperNova, two other photosensitizers used for cell ablation...illuminated with green light. They found that KillerRed required longer exposure times (5 minutes vs 10 seconds...* also had higher photostability than KillerRed, with KillerRed reaching greater than 75% photobleaching...modified with the addition of two iodine residues. Compared to MG, MG-2I has increased ROS generation when...wavelength to 666 nm, which is within the near-infrared (NIR) spectrum (>620 nm). Above is a brief schematic...bleaching after longer exposure times. Additionally, KillerRed requires mitochondrial targeting to increase cell...

...SunTag system, named after the "stellar explosion SUperNova", is a synthetic scaffold that recruits up to ...will appear yellow, but a translated mRNA will be red. This system achieves great specificity and resolution... and that fluoresce in the green (500-550nm) and red (570-620nm) portions of the spectrum. PhD student...transitions from emitting orange (λ= 565nm) to far-red (λ= 662nm) fluorescence after exposure to blue-light...Biology (2014) represent the newest versions of both a red- and a green-light switchable bacterial TCS system...aaRS pairs are delivered via a pseudotyped baculovirus system, which is advantageous compared to other viral... Good tools for measuring intracellular pH are required in order to determine the relationships between...