...interest insertion site.   The Sleeping Beauty transposon system The Sleeping Beauty (SB) transposon system is...that can translocate within the genome. The Sleeping Beauty transposase gains its name from the fact that...changes. The basic functional version of the Sleeping Beauty transposon (SB10), developed in 1997, continues...efficiencies of integrating viral vectors. New Sleeping Beauty transposase vectors Even more recently, Eric...constitutively or regulated by doxycycline. The Sleeping Beauty transposase is proving an exciting tool for...presented results from a clinical trial using the Sleeping Beauty transposase as a nonviral vector for CD19-directed...  References 1. Kowarz E, et al. Optimized Sleeping Beauty transposons rapidly generate stable transgenic...

...research tools: Sleeping beauty, PiggyBac, and Tol2.  Sleeping beauty Sleeping Beauty is a synthetic transposable...in fish (Sandoval-Villegas et al., 2021). Sleeping Beauty’s preferred target site for integration is TA...integration efficiency decreases with cargo size. Sleeping Beauty has a close-to-random integration profile in...transposition activity (Voigt et al., 2016). Sleeping Beauty plasmids  piggyBac Although its name suggests...islands, and DNaseI hypersensitivity sites. Like Sleeping Beauty, piggyBac has an integration efficiency in ...caused albinism (Koga et al., 1996). Unlike Sleeping Beauty and piggyBac, Tol2 has a weak consensus sequence...integration in human cells than piggyBac and Sleeping Beauty. Minimal Tol2 or miniTol2 is a truncated version...

...himself.   Sleeping Beauty, a transposon system from ancient fish Surprisingly, Sleeping Beauty is not only...awakened from a long evolutionary sleep,” it was aptly named Sleeping Beauty (Ivics et al., 1997). We have... in rooms such as Groucho, Sonic Hedgehog, Sleeping Beauty, Cookie Monster, Spaghetti Squash, Tinman, ...wheel but also a biological transposon system. Sleeping Beauty is a synthetic transposon system that can be...this transposon system? Scientists can use Sleeping Beauty transposons as a non-viral system to integrate...

... About Other Genome Engineering Tools like Sleeping Beauty Mutase Find the Ideal CRISPR Software for Your...

...knock-in tools for human therapeutics (e.g. using Sleeping Beauty). Despite their diverse utility, DNA transposons...

...Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase Read up on Genome Engineering with...