...expression cassette. (b) pCAG-EGxxFP target plasmid and pX330-sgRNA plasmid (Mashiko et al. 2013). The pCAG-EGxxFP...-cloning site (MCS) between EGFP fragments. The pX330 plasmid contains humanized Cas9 expression cassette...pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA sequences into HEK293T...plasmid along with the individual gRNAs in their PX330 plasmids (or similar plasmids for gRNA expression...system. However, it is easier to directly inject the pX330 plasmids used in the validation assay (above) and...

...gRNAs into spectinomycin-resistant plasmids pX330S-2 to pX330S-(last gRNA number). The 5’ most gRNA is cloned...gRNA from a single promoter using a plasmid like pX330? Unfortunately, the short answer is no. Unless you... from the Ventura lab. pX333, a modification of pX330, contains humanized wtCas9 and two U6 promoters....expressing 4 gRNAs, you’d use pX330A-1x4; for 6 gRNAs, you’d use pX330A-1x6. This customization means ...

...over the years on their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9... be cloned to create a chimeric guide RNA. Find pX330-U6-Chimeric_BB-CBh-hSpCas9. pSpCas9(BB)-2A-Puro ...

...sequence into a Cas9-containing plasmid such as pX330. This cloning strategy not only makes it easy to...

...LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9-LSD1 for targeted removal of H3K4me1/2 and...