... containing alternating MS2 and PP7 sequences (sgRNA-Sirius-4X(MS2-PP7)) that result in yellow fluorescence...created another sgRNA containing the PP7 hairpin sequence (sgRNA-Sirius-8XPP7) to recruit GFP to the loci of... sgRNA-Sirius-8XMS2, sgRNA-Sirius-8XPP7, and sgRNA-Sirius-4X(MS2-PP7) showing recruitment of different... that contain two hairpin sequences (either MS2, PP7, or boxB) that each recruits a different fluorescent... Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences inserted into the sgRNA generates...or red colors for labeling. A combination of MS2, PP7, or boxB sequences in the same sgRNA will generate... the gRNA. Because of the success of the MS2 and PP7 RNA hairpin sequences in CRISPRainbow, they knew ...

...Hairpin Blue MS2-MS2 Green PP7-PP7 Red boxB-boxB Cyan MS2-PP7 Yellow PP7-boxB Magenta boxB-MS2 ... White boxB-MS2-PP7 Despite the success of Ma et al.’s approach, fluorescently labeled Cas9’s come...

...binds to the coding region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) an RFP protein that binds...system are achieved by using multiple copies of the PP7 and MS2 coat protein binding sites to tether many...

...Root and Doench labs utilized tracrRNAs with two PP7 stem loops to recruit additional transcription factors...

...binds to the coding region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds...great specificity and resolution by using 6-12 phage PP7 coat protein binding sites and 24 copies of the MS2...