... of the AAV and lentiviral preps we have released in the last few months: Lentiviral preps for expressing...plasmids into lentiviral preps to speed up COVID-19 research even further. The following lentiviral preps are...pAAV-Ef1a-fDIO mCherry Karl Deisseroth 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP Brandon...pAAV-hSyn-DIO-mCherry Bryan Roth 50457 AAV1 pAAV-hSyn-DIO-EGFP Bryan Roth 114472 AAVrg pAAV-hSyn-mCherry Karl...pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry Connie Cepko 83898 AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 Gordon Fishell... GFP dependent Cre and FLPo And more!  New lentiviral preps SARS-CoV-2 protein expression The Krogan...2xStrep-IRES-Puro: Expression of the SARS-CoV-2 ORF3a Lentiviral preps for expressing the E protein (pLVX-EF1alpha-SARS-CoV...

...anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in ...lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a...gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely...these viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor...receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control of the human synapsin promoter. Purpose... Purpose of this AAV pAAV-hSyn-DIO-hM4D(Gi)-mCherry can be used to induce neuronal silencing in vitro ...Aschauer et al., 2013). Expression of hM4D(Gi)-mCherry from this virus is cre-dependent, meaning that ...

...in the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...Concentrated lentiviral preparations (“-LVC” catalog items) are generated from lentiviral preparations...being frozen. Specifically, lentiviral particles are collected from the lentiviral preparation by precipitation...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...Browse our AAV vector collection. Lentivirus Production Lentiviral preparations ("-LV” catalog items)...of the transfer plasmid.   Lentivirus resources Browse lentiviral preps available from Addgene. After...through how we produce both our AAV preps and our lentiviral preps.   Viruses are generated using standard...

... with mCherry and you already have a transgenic mouse line that contains a Cre-dependent mCherry cassette...with mCherry controlled by a neuron specific promoter or you could breed your Cre-dependent mCherry line... and requests are still pouring in! While our lentiviral service is going strong, the AAV service has ...incredible growth this year. pAAV-hSyn-DIO-hM4D(Gi)-mCherry was the top requested AAV prep for the 2nd year...you need would be to inject your Cre-dependent mCherry mouse with the pmSyn-EBFP-Cre AAV. Because Cre ...controlled by the synapsin promoter, it and therefore mCherry will only be expressed in neurons. Indeed, this...

...promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the weaponized... A novel RNA targeting system Heat-inducible lentiviral Car constructs Anti-GFP [N86/8R] ready-to-use...of print. PMID: 36690762.   Heat-inducible lentiviral CAR constructs by: Brian O'Neill  A clever system...crux of the system is a set of heat-inducible lentiviral CAR constructs, the most basic of which is depicted..., inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the...

...deaminases like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and ...expresses eGFP to indicate reporter abundance, and mCherry fluorescence is activated when APOBEC-Cas9n-UGI-mediated...activity edits out a frame-shift mutation within the mCherry reporter. Quantification of editing efficiencies...efficiencies simply involves tracking the ratio of mCherry-positive to eGFP positive cells through fluorescence...

...designed by putting together the coding sequence of a mCherry fluorescent protein followed by a stop codon and... flow cytometry is used to sort populations by mCherry and GFP expression to check for ABE’s. b) Quantification... 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker...

...Blog Learn More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction Additional... individually overexpressed and available for lentiviral packaging and delivery to target cells as a pooled... Fig. 1: Barcoded TF library was packaged in lentiviral particles and used to transduce human embryonic...The pooled library comes either with or without mCherry and GFP fluorescent controls. Alternatively, you...

...to create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed... ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules mCherry-alpha-tubulin...mito-BFP mCherry-Drp1 (GTPase in division) GFP-Mff (outer membrane protein) Late Endosome mCherry-Rab7A... MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with just the...collection of plasmids comprising the multiple lentiviral expression (MuLE) system from the laboratory ...Pre-constructed Entry vectors containing Cas9, EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or... combinations of genetic elements in a single lentiviral vector provides a powerful tool to modify mammalian...

...MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect...Insect pUltra P2A and T2A Lentiviral Ac5-STABLE2-neo T2A Insect   For the co-expression of multiple....) PubMed. 3. Highly Efficient Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et...

...infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation...plasmids for purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes...

...p2A-ferritin-p2A-mCherry Mammalian Expression 74308 pcDNA3.0-Magneto2.0-p2A-mCherry Mammalian Expression...the authors packaged it into lentivirus and used the resulting lentiviral library to generate a population...Science. 2013. PubMed PMID: 23328393 Wu lab human lentiviral CRISPR library     CRISPR pooled libraries enable...Library. This complex second-generation CRISPR lentiviral library targets nearly all human protein-coding...from the CDC and cloned into a third generation lentiviral transfer vector. Each gene has been FLAG-tagged...and named it Magneto 2.0. Using Magneto2.0-p2A-mCherry constructs, the lab verified that Magneto2.0 was...Expression 74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...

...2019). This dual lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with ...by the Day Lab, who developed neuron-optimized lentiviral vectors for in vitro and in vivo CRISPRa (Savell...neuronal physiology. The Day Lab’s neuron-optimized lentiviral vectors can be found here. High-throughput screening...Cas9 activators can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV... such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression of sgRNA (top...

...module and the SspB-mCherry-p60 katanin module, which are also available as lentiviral plasmids if you want... activity. EB3N-VVDfast-iLID (anchor) and SspB-mCherry-p60 (katanin) are recruited to microtubules under...

...facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in...assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation with assist...