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  1. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...* also had higher photostability than KillerRed, with KillerRed reaching greater than 75% photobleaching... and in vivo.  Read on to learn more about this killer illumination technique! What is a photosensitizer...al also compared MG-2I-dL5** head-to-head with KillerRed, a genetically encoded red fluorescent photosensitizer...illuminated with green light. They found that KillerRed required longer exposure times (5 minutes vs 10...bleaching after longer exposure times. Additionally, KillerRed requires mitochondrial targeting to increase cell...
  2. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...8862819. 2. Bahassi, EM., et al. “F plasmid CcdB killer protein: ccdB gene mutants coding for non-cytotoxic...Positive-selection vectors using the F plasmid ccdB killer gene”. Gene. 1994 Oct 11;148(1):71-4. PubMed PMID...
  3. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...CD8b-PerCP-Cy5.5), which is present on cytotoxic T killer cells but not dendritic cells. Using a graph displaying...displaying both stains, I can quickly separate T killer cells (CD8+) from dendritic cells (CD11b+) using...
  4. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ..., IgG antibodies bind to a pathogen and natural killer (NK) cells expressing Fcγ receptors binds to IgG...
  5. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...various cell populations; for example, CD8+ T (“T killer”) cells. Typically, you would label the CD8 co-...
  6. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...versus CD19 to separate B cells, T cells and natural killer (NK) cells.   Tips for flow cytometry success...
Showing: 1 - 11 of 11 results