...lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute and was designed ...PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries ...take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR v2 has only been available...

...Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015)  Reference Feng, S., Sekine, S., Pessino, V. et...producing fluorescent signal. Image modified from Feng et al. 2017 under CC-BY license. Many split-FP...fluorescent proteins for endogenous protein labeling. Feng et al. Nat Commun. 2017 Bo Huang mNeonGreen3 mNG3K...protein labeling and signal amplification. Zhou S, Feng S, Brown D, Huang B. PLoS One. 2020 Bo Huang Yellow...fluorescent proteins for endogenous protein labeling. Feng et al. Nat Commun. 2017 Bo Huang sfCherry3 Improved...visualization of endogenous proteins and synapses. Feng S et al. Commun Biol. 2019 Bo Huang Near-Infrared...protein-protein interactions in living cells. Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y. Commun Biol. 2021...

...resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, Y., Dong, A....which helped researchers visualize norepinephrine (Feng et al., 2019). But this first generation of tools...A., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., ...

...graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of customizable DNA-binding...new. Now, Konermann and her colleagues including Zhang and Mark Brigham have taken the technology to another...literally at the flip of a (blue) light switch. Zhang’s team devised the plasmids now available in Addgene...introducing the optogenetic tool, Konermann and Zhang showed they could package LITEs in viral vectors...specific epigenetic modifications in the genome,” Zhang said in MIT’s press release. In an accompanying ...

...example, the authors Patrick Hsu, Eric Lander, and Feng Zhang acknowledge the role of data and materials repositories...

...with all these questions, Dr. Le Cong and Dr. Feng Zhang developed a Google Forum to allow scientists ...more Q&As which focus on genome editing using the Zhang lab's CRISPR/Cas plasmids. Designing your CRISPR...Double nickase' is a new system, developed by the Zhang lab, which has comparable efficiency to the optimized...nickase system, including one recently from the Zhang group. Learn more here. Q2: When designing oligos...without the G. Because the G is only one base, the Zhang lab usually adds it when they order the oligo. If...

... Speaking of CRISPRs, we were excited to have Feng Zhang (of the Broad Institute of MIT and Harvard and...depositing scientist – stop by our booth to say hi! Zhang was awarded one of this year’s SfN Young Investigator...

...screening - An excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production...using CRISPR/Cas Systems Forum- Moderated by the Feng Zhang lab, this is a useful place to ask experimental...Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional...

...screening. They and their colleagues including Feng Zhang used the GeCKO library to identify genes essential...titer due to the smaller size of the plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single...Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science. 2013 Dec 12. (PubMed). Genome-Wide Recessive...

...in December, Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they ...perfect complement for one conducted earlier by the Zhang lab for GeCKO, a lentiviral CRISPR library enabling...enabling genome-scale, knockout screening). As the Zhang lab's Nature paper reports, “Here we have shown ... Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O. Cell. 2014 Feb 27;156(5):935-49. doi... Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2015 Jan 29;517(7536):583-8. doi: 10.1038...

...: 237-243. PubMed PMID: 20882. Mei, Yuan, and Feng Zhang. "Molecular tools and approaches for optogenetics... 28772120. PubMed Central PMCID: PMC5830081. Zhang, Feng, et al. "The microbial opsin family of optogenetic...membrane potential in response to visible light (Zhang et al., 2011). What do microbial rhodopsins and ...electrophysiological methods (Deisseroth, 2011; Mei and Zhang, 2012; Towne et al., 2016). Optogenetic neuromodulation...neural activity (Bernstein and Boyden, 2011; Mei and Zhang, 2012). For example, blue light activates channelrhodopsins...

...little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple ...a smaller, but equally potent Cas9 protein, the Zhang lab took a different approach. They analyzed over...compare the off-target effects of SpCas9 and SaCas9, Zhang’s group used an approach called BLESS (direct in ... test the efficiency of AAV-SaCas9 in vivo, the Zhang lab created an all-in-one SaCas9 and sgRNA construct... Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR... Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing with CRISPR-Cas13. Science ... Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O (2014) Crystal Structure of Cas9 in ...

...  Improved nucleic acid sequence detection Feng Zhang’s lab recently developed a platform called SHERLOCK...

...names in the CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene...Addgene was mentioned specifically in Dr. Zhang's introduction. His willingness to share reagents so freely...

...study author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future, it ...

...incompatible to genome editing in human cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii...

...plasmids   Cytosine deaminase for RNA editing Feng Zhang’s lab previously developed a programmable adenosine-to-inosine...

...s41587-023-02050-w Yi, Z., Zhang, X., Wei, X., Li, J., Ren, J., Zhang, X., Zhang, Y., Tang, H., Chang, X....Hong, M., Luan, C., Gao, H., Ru, G., Guo, X., Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa...., Qu, C., Zhu, Y., Guan, Y., Wang, L., Liu, M., Feng, B., Song, G., … Li, D. (2024). Adenine transversion...., Cui, S., Li, H., Li, G., Zhou, J., Yuan, Y., Zhang, H., Shi, L., Yao, X., & Yang, H. (2023). Programmable... H., Wei, Z., Liu, X., Li, Y., Li, S., Liu, Y., Zhang, X., & Bi, C. (2024). Glycosylase-based base editors...

...the MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas...In addition to publishing in Science, Professor Zhang deposited the associated plasmids with Addgene. ...

...Note: Cpf1 is also called Cas12a. In 2015, Feng Zhang’s lab characterized two Cpf1 nucleases, distant...regulatory elements. To solve this problem, the Zhang lab has previously used SaCas9 (1053 aa/3.2 kb) ...SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. Cpf1 Is a Single RNA-Guided Endonuclease of a...