...post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient The first...fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression...Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels...levels of genetic control. What is FLP FRT and how does it work? FLP recombinase, which is named after ...Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase recognizes...(LDB) to the C-terminus of FLP or Cre (pCAG-CreERT2 #14797). These Cre/ FLP versions are retained in the...Andrea et al. 2010. “An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48(8): ...

... we are excited to add Flp-expression AAV to our recombinase AAV inventory. Flp is another useful recombinase...populations. We now offer a Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV. See our recombinase...detection PSAM4 GlyR for chemogenetic inhibition Flp expression for intersectional studies And more! ...Serotype Name 55637  AAVrg pAAV-EF1a-Flpo 87306  AAV1 AAV pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors...55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1  pAAV-EF1a-Flpo Chemogenetics Plasmid Serotype...

...with low cross-reactivity, as well as low toxicity. FLP/FRT recombinases (from Saccharomyces cerevisiae) ...with flies heterozygous for tubulin-GAL4. For Dre, Flp, KD, R, B2 and B3, approximately 50% of offspring...shows that the yeast recombinases KD, B2, B3 and FLP can be used together in Drosophila as a toolkit for...

...expressing both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469), is also available in AAV1. Plasmid...expression. Plasmid 75470 is now available in AAV1 for Flp-dependent expression of ChR2(H134R) bound to an mCherry...pAAV-EF1a-C-CreintG Connie Cepko 75469 AAV1 pAAV-EF1a-Flp-DOG-NW Connie Cepko Viral vectors coming soon...targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral preps SARS-CoV-2 protein...

...in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-directed... conceptually similar to Cre-lox, with flippase (Flp) and the short flippase recognition target (FRT) ...being analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective alternative to...

...of the site-specific recombinases (SSRs) Cre and FLP. SSRs bind DNA at target sites to induce site specific... events: Cre recombinase binds loxP sites, while FLP binds FRT sites. When a DNA sequence is flanked by...and a pair of FRT sites and include both Cre and FLP recombinases.   Figure 1 (modified from Schnutgen...

...transgenic models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent...sequences, lox (recognized by Cre) or frt (recognized by Flp), to either flip or excise a gene that is located...leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify the amount of transgene...expression can occur in cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and ...

...pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9...our calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several new options... Serotype Name 87306 AAVrg, AAV5 AAV pEF1a-DIO-FLPo-WPRE-hGHpA    Additional resources on the Addgene...

...DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo (55634-AAV1) pAAV-EF1a-Flpo (55637-AAV1) Viral...collection. Of note, we are excited to release a Flp-dependent Cre AAV in AAV9 and AAVrg. See our new ...

...expression in Cre-expressing cells. hSynap-FLP: Synapsin-1 promoter, FLP-dependent expression GFAP: Glial fibrillary...

...Specificity can be achieved using specialized promoters, Flp or Cre dependent gene switches, and serotype selection...cell-specific gene expression and viral infection. Cre or Flp dependent switches control gene expression via switches...

.... It’s an alternative to the conventional Cre or Flp recombinases used to excise markers. It has the advantage...

...common, existing tools for fly research that use Flp recombination. In addition to the XFP in the construct...Drosophila melanogaster and is under the control of Flp recombinase. The XFPs in Flybow are membrane-tethered...

...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT ...-responsive CreERT, the coupling of Cre-lox with FLP-FRT recombination to generate additional recombination... placed under different promoters, Brainbow and Flpbow can be used to label distinct cell populations....

... neurons or you might use it in combination with Flp-FRT. Additionally, you could use the retrograde form...

...classical genetic tools, for example Cre/loxP or Flp/FRT, with AAV vectors are common approaches to control...

...pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1  pAAV-EF1a-Flpo Calcium...

...Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff/Fon-sRGECO ...

...retrograde recombinase-expressing vector (e.g. Cre or FLP) to transduce the projection pathway neurons and ...

...on the Addgene Blog Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Interview: Ed Boyden...