... (TMP) Cas9 fused to destabilizing domains (Cas9-DHFR) Gene editing, transcription Zhou et al., 2020...

...fast-reacting version of SNAP-tag called SNAPf. eDHFR: The Virginia Cornish and Michael Sheetz labs demonstrated...proteins fused with E. coli dihydrofolate reductase (eDHFR) could be labeled with fluorescent derivatives of... showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent binding of the TMP-tag ligand...SNAP-tag 182 Benzylguanine (BG) Many Easy eDHFR 159 Trimpethoprim  (TMP-tag) Few Easy ...application, SNAP-tag, CLIP-tag (both 182 aa), and eDHFR (159 aa) are smaller than HaloTag (295 aa) – for...