...Targeting pEF-BFP with a cytidine deaminase base editor results in shift in emission spectra from BFP to GFP....293 cells with the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for the BFP-to-GFP conversion...deaminase base DNA editor. Specifically the BFP mutant (BFPH66) contains a histidine at amino acid 66. This...an integrated GFP reporter that is converted into BFP (blue fluorescent protein) upon CRISPR/Cas9 homology...Glasser et al., 2016). For TREE they engineered a BFP variant that undergoes conversion to GFP after being...from the Wang and Brafman lab uses an engineered BFP variant that converts to GFP after a base editing...nature24644 Glaser A, McColl B, Vadolas J (2016) GFP to BFP Conversion: A Versatile Assay for the Quantification...

... of interest makes pmSyn-BFP-Cre highly modular. Further uses of pmSyn1-EBFP-Cre Of course there are also...requested AAV that became available in 2017 is pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over...over 150 orders since coming online! pmSyn1-EBFP-Cre Available in serotypes AAV1, AAV5, and AAV retrograde... your Cre-dependent mCherry mouse with the pmSyn-EBFP-Cre AAV. Because Cre expression is controlled by...re excited to see how you continue to use pmSyn1-EBFP-Cre and other viral vector tools in the years to... pmsyn1-ebfp-cre...

... want to design a genetic FLEx switch that turns BFP expression off, while turning on mCherry expression...successfully work, the cassette would need to contain the BFP coding sequence in the sense orientation, followed...Nat Biotech 2003): Basic FLEx switch will express BFP in the absence of, or mCherry in the presence of ...specifically drives expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful...

...nanobody cross-reacts with BFP, sender cells expressing surface BFP generated BFP-labeled receiver cells....

... range).  By mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP) were derived...will pass blue light to the detector/camera, then BFPs are of no use to you. When using more than one FP... light. Photostability can be as short as 100ms (EBFP) or as long as 1 hour (mAmetrine1.2). However, for...

...FACS, gating for the top quartile (or higher) of BFP and GFP producing cells. After FACS, return the cells...cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP plasmid and 954 ng CMV-gvpNJKFGWV plasmid into 70%...contains the major structural gene gvpA marked with EBFP2 and the second cassette contains the P2A-linked ...

...PubMed Central PMCID: PMC5495063 Kan et al. use a GFP-BFP conversion system to assess precision genome editing...

...hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI restriction...

...Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules...-Rab5 BFP-Rab5 GFP-Rab5B Vacuolar & Budding Compartment GFP-Rab4B Mitochondria mito-BFP mCherry-Drp1...dCas9s with one of three fluorescent proteins (GFP, BFP and mCherry), the authors were able to visualize ...

...that each recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of interest. Pairs...

...hairpin sequences, which recruit fluorescent proteins BFP, GFP, and RFP fused to the respective hairpin binding...

...derivatives) Y145F Increases quantum yield for BFP Y145A and H148D Stabilizes the structure of Cerulean... Y66W, S72A, Y145A, H148D, N149I, M153T, V163A EBFP F64L, S65T, Y66H, Y145F   Table 2: Functional...

... family members (superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be ...

...Color Base FP Description Article PI Blue EBFP2 EBFP2(1-10) and Capri(1-10) for use with GFP(11) Multiplexed...fragments, even from a different base FP (for example, EBFP2(1-10) can be used with GFP(11)). Some of the articles...

...of a miRNA target site. One fluorescent protein (EBFP2) is expressed in an unregulated fashion and thus...it will repress expression of mKate2 relative to EBFP2. Weiss’s group used these reporters to define a ...

...fluorescent proteins for imaging with this set are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2...

...luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP, and T2A-TurboGFP-PEST), and small epitope...

... Tethering partner Subcellular localization 1 TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean...

... by Puromycin, Zeocin®, or nuclear expression of BFP2.     Figure 1: CROPseq-multi uses two sgRNAs...

...non-glycosylated monomeric blue fluorescent protein, secBFP2, for studies in the eukaryotic secretory pathway...