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Showing: 1 - 9 of 9 results
  1. Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

    Type
    Blog Post
    Published
    Dec. 11, 2017, 2:02 p.m.
    ...Plasmid 1: B. subtilis knock out and knock down libraries. Listen to Our segment on the B. subtilis libraries...you'll learn about new libraries for studying B. subtilis biology, plasmids for enhancing iPSC production... b. subtilis...to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital Media Production Specialist at ...
  2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...introduced into the B. subtilis genome. Mobile-CRISPRi is then transferred from B. subtilis to the bacterium...such as a B. subtilis, or a narrow range of bacterial species, such as the human gut bacteria B. thetaiotaomicron...bi-parental mating. Following excision from the B. subtilis genome, the Mobile-CRISPRi ICE element forms ...integrative and conjugative elements in Bacillus subtilis (ICEBs1) system. ICEBs1 carries the genes needed...integrative and conjugative element in Bacillus subtilis." Plasmid 86 (2016): 14-25. PubMed PMID: 27381852...
  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...recently deposited 2 Bacillus subtilis libraries with Addgene – the B. subtilis Single Gene Deletion Library—Kanamycin... the podcast Segement on the pPSU Plasmids B. subtilis knockout and knockdown libraries The Gross ...CRISPRi Essential gene Knockdown library. Although B. subtilis has a fully sequenced genome, many applications...used to advance our collective understanding of B. subtilis gene functions and interactions. The single-gene...-gene deletion library is derived from the B. subtilis 168 strain and contains knockouts for all 3984 ...genes identified or proposed to be essential in B. subtilis 168. Each individual strain includes a xylose-inducible...27238023 Listen to the podcast segment on the B. subtilis libraries Transcription factor fusions elucidate...
  4. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...proteins. pCri vector pCri-13a, designed for use in B. subtilis, contains the MISTIC (membrane-integrating sequence...Tags. Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids...than E. coli, specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory...
  5. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...Sciences 113.13 (2016): 3509-3514. Tikhonova, Elena B., et al. "A transcription blocker isolated from a ...
  6. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...j.plasmid.2023.102684 Shareck, J., Choi, Y., Lee, B., & Miguez, C. B. (2004). Cloning Vectors Based on Cryptic... T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L....possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...
Showing: 1 - 9 of 9 results