...amino acid) AviTag sequence in a protein and adds biotin to a lysine residue within the AviTag. However,...carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification (see ...ligase will biotinylate the lysine residue in the AviTag. As a side note, we found that adding a TEV linker...linker dramatically increased accessibility of the AviTag and HisTag, which were “buried” in a few of our... fusion proteins - but it would remove both the AviTag and HisTag. We were surprised that we couldn’t...

...biotinylation. This 13 amino acid sequence is termed AviTag. Using AviTag, one can label a protein of interest with...Scientific's Biotinylation Reagent Selection Tool. AviTag and BirA Proteins can also be biotinylated by exploiting...with biotin by simply creating a fusion in which AviTag is added to either the N or C terminus of the protein... BirA has been shown to effectively biotinylate AviTag expressing proteins in bacteria, yeast, insect ... BirA. You can find plasmids that contain both AviTag and BirA at Addgene! Oligonucleotide labeling Biotin...

...commonly used for in vivo biotinylation via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed...followed by the desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the...

... and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann lab developed...