A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
Type
Blog Post
Published
May 7, 2020, 1:15 p.m.
...smaller version, ABE8.x-m, showed comparable editing behavior to ABE8.x-d. Overall, ABE8s showed a median...created ABE8 constructs using a catalytically “dead” S. pyogenes Cas9 (dC9-ABE8.x-m/d). The dC9-ABE8.x-m/...adenine base editors, called ABE8 (Gaudelli et al., 2020). The generated ABE8s needed to produce 3 concurrent... and TadA7.10, they designed one set of ABE8’s similarly (ABE8.x-d). In order to assess the effect of ...
The ABE8.x-d version carries a fusion of wild-type TadA and the evolved TadA region.
The ABE8.x-m version...500 base-pair smaller than ABE8.x-d.
This resulted altogether in 40 new ABE8 variants. When comparing ...: NG) to create NG-ABE8.xm/d and the S. aureus Cas9 (PAM: NNGRRT) to create Sa-ABE8.x-m/d. With these ...