...polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target more than...Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing...PMC5972399. 12. Molla, K., Shih, J. & Yang, Y. Multiplex CRISPR-mediated base editing in rice. In Genome...

...et al., 2019). Because of its ability to target multiple gene loci simultaneously, CRISPRa can also be ...(Chavez et al., 2015)) are good choices across multiple cell lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3...Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods 13:563–567 . https://doi....

...assembly is well known for allowing easy assembly of multiple linear DNA fragments, but can also be used in ...biology, mainly because of the ease in assembling multiple fragments in one step with no scar sequences remaining...Whether it is for routine cloning, assembly of multiple fragments, or synthetic biology, you should consider...

...hotspot for dystrophin, exons 45-55, there are multiple common deletions that maintain the protein’s reading...skipping? For such a therapy to be successful, multiple requirements must be met. First, CRISPR would ...Thakore PI, Majoros WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome editing for correction...

... added to the reaction for easy detection. The multiple biotin binding sites found on avidin are also ...during PCR using a biotin labelled dUTP. There are multiple types of biotin labels that can be added to an...Biotin labelled oligonucleotides can be used in a multitude of applications including but not limited to in...

...with different ITAM/SH2 reporter pairs allow multiplexed measurement of different RTKs. Although this ...for lactate developed using a high-throughput multiparameter screen (Koveal et al. 2022). Lactate is a key... here!   Koveal D, et al. A high-throughput multiparameter screen for accelerated development and optimization...

.... Digestion with enzyme “B” detects primer multimerization (purple) which is evident by an increase in...CRISPR-induced genome modifications. Occasionally, multimerization of the PCR primers can cause duplication of...particularly relevant in cases where a large plasmid with multiple functional regions is used as template for the...

...is necessary or desirable to control or look at multiple genes at the same time.  The 3 binary systems ...analysis in Drosophila or for precise control over multiple genes in synthetic systems. Recent work has even... Multiple Authors...

...about 100 bps into the run, it got “dirty”, with multiple sequence traces overlapping each other! When the... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering...

...counts making them fast and viable options for multiplex sequencing in many laboratories. Nevertheless,...DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic...dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to...

... protein because the antibodies will recognize multiple epitopes on the protein. However, each animal ...advantage of flow cytometry is the ability to use multiple fluorescent signals (up to a dozen or more!) to...relative or absolute amount of the expression of multiple proteins on the same cell simultaneously as well...

...KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv ...Cynthia Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers...

...way to increase CRISPR editing efficiency across multiple CRISPR enzymes. Plasmids for CRISPR coselection...segment on regulating telomere length with TZAP Multiplexible plant genome engineering toolkit The Voytas ... well as Cys4, tRNA, and ribozyme systems for multiplex gRNA expression. Expression of toolkit components...

... they have developed this further to provide multiplexed detection, higher sensitivity, and more. Read...lab compared the efficiency and specificity of multiple wild-type and engineered variants of Cas nucleases...

... which display sophisticated interactions with multiple other proteins. Again, little to no change in ... place to start. These tags are easy to use in multiple common applications. Due to their limited effects...

...less experienced grads Manage a project involving multiple scientists, create and stick to timelines Be a...another field maybe, and publish Join a lab that has multiple collaborations, even with Industry labs Work on...

...feedback and input on multiple topics every time you meet.  Seek feedback in multiple formats      Ask ...

...e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus to ensure their...elegans gRNA sequences. You can also filter by multiple keywords that are separated by a single space ...

...protein purification through simple and efficient multiplexing. pCri plasmids are divided into two sets, each...schematic below), enabling a researcher to test multiple tags for one protein quickly and easily. These...

...both colors allows for increased options for multiplexing in experiments, including ones that study alpha2...Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo...